Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550366..3551036 Replicon chromosome
Accession NZ_CP071127
Organism Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JY387_RS16650 Protein ID WP_003899954.1
Coordinates 3550366..3550710 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JY387_RS16655 Protein ID WP_003899955.1
Coordinates 3550707..3551036 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY387_RS16620 3545439..3546299 + 861 WP_003416628.1 alpha/beta hydrolase -
JY387_RS16625 3546274..3546789 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JY387_RS16630 3547029..3547322 + 294 WP_003416635.1 hypothetical protein -
JY387_RS16635 3547610..3548899 + 1290 WP_003416640.1 ATP-binding protein -
JY387_RS16640 3549246..3549680 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JY387_RS16645 3549683..3550135 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JY387_RS16650 3550366..3550710 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JY387_RS16655 3550707..3551036 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JY387_RS16660 3551273..3552534 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JY387_RS16665 3552756..3554017 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JY387_RS16670 3554290..3554637 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JY387_RS16675 3554634..3555254 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3549683..3577028 27345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T195384 WP_003899954.1 NZ_CP071127:3550366-3550710 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T195384 NZ_CP094696:1698112-1698585 [Staphylococcus simulans]
ATGGGAGTTTACGAAGATTTATGTATCGCTAACGATTGGGTTGAGATTGAGGAGACTGATCGTTTACCTAGTTTCCAACC
AGGGTTTTACAGAAATGGAAAAATCTATATTAAAAGCAGTCTTTCCGAAACACGCAAAGCTGAAGTTCTCTATGAAGAAT
TAGCTCACCATAAACTTACGTACGGAAACATACTTGATGAATCAAGCTTCAATAACCGTAAATTCGAAAATTACGCACGA
AGACATGGTTATGAAAATTCTATATCTTTAAACAAGATTATAGACGCATATAAATACGGAGTAAGTAGCTTACATGAATT
TGCTGAATATGTTCAATTAAGCGAAGAATACGTACATACAGTTTTACAACATTACAAAAACAAATTCGGTTTATCAACCT
GCCACAATGGTTATCTTATTCGTTTTGAGCCGTTGCAGGTTTTTAAATATAAAAATTTAAATGAAGGAGATTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT195384 WP_003899955.1 NZ_CP071127:3550707-3551036 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT195384 NZ_CP094696:1697766-1698098 [Staphylococcus simulans]
ATGAGAACAAATGATGAAATAATCACAATAATAAAAACAACGTTAAAAGAACAAAATATGTCGCTTAGTGAATTAGCTCG
TCGTGTAGGAATGGCTAAATCAGCTGTATCGCGTTATTTAAACTTAACTAGAGAGTTTCCATTGAACCGTGCGGAAGATT
TTGCAAAAGCACTTCATATTAGTACAGAATATTTACTTGGTTTTACAGAAAGTGAACAAAAAGAGCAAGAACAAGACACT
ATGGCCGCTCATTTTGACAAAGAAGGTCTAACAGAAGAGGAAATTGAAGAAGTTAATAAGTTTATTGAATGGGTTAAGAA
TAGAGATAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References