Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4237571..4238241 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BK419_RS19800 Protein ID WP_003899954.1
Coordinates 4237897..4238241 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BK419_RS19795 Protein ID WP_003899955.1
Coordinates 4237571..4237900 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS19765 4232986..4234020 + 1035 WP_003416786.1 IS30 family transposase -
BK419_RS19775 4234267..4234476 - 210 WP_003416778.1 hypothetical protein -
BK419_RS19780 4234644..4235909 + 1266 WP_003902423.1 hypothetical protein -
BK419_RS19785 4236069..4236689 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
BK419_RS19790 4236686..4237033 - 348 WP_003899956.1 DUF2384 domain-containing protein -
BK419_RS19795 4237571..4237900 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BK419_RS19800 4237897..4238241 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BK419_RS19805 4238472..4238924 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BK419_RS19810 4238927..4239361 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BK419_RS19820 4239470..4240731 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BK419_RS19825 4241066..4242355 - 1290 WP_003416640.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4219814..4243935 24121


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T68342 WP_003899954.1 NZ_CP017920:c4238241-4237897 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T68342 NZ_CP017920:c4238241-4237897 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT68342 WP_003899955.1 NZ_CP017920:c4237900-4237571 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT68342 NZ_CP017920:c4237900-4237571 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References