Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) TscAT/-
Location 2069025..2069563 Replicon chromosome
Accession NC_002745
Organism Staphylococcus aureus subsp. aureus N315

Toxin (Protein)


Gene name TscT Uniprot ID A0A0H3K0C1
Locus tag SA_RS10500 Protein ID WP_001078344.1
Coordinates 2069025..2069351 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name TscA Uniprot ID A0A0H3JSN0
Locus tag SA_RS10505 Protein ID WP_001058495.1
Coordinates 2069354..2069563 (-) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SA_RS10475 (SA1825) 2064249..2064590 - 342 WP_001190615.1 hypothetical protein -
SA_RS10480 (SA1826) 2065040..2065681 - 642 WP_001047698.1 hypothetical protein -
SA_RS10485 (SA1827) 2065678..2066058 - 381 WP_000356942.1 hypothetical protein -
SA_RS10490 (SA1828) 2066368..2068077 - 1710 WP_000447473.1 DUF927 domain-containing protein -
SA_RS10495 (SA1829) 2068091..2068960 - 870 WP_001002689.1 primase alpha helix C-terminal domain-containing protein -
SA_RS10500 (SA1830) 2069025..2069351 - 327 WP_001078344.1 DUF1474 family protein Toxin
SA_RS10505 (SA1831) 2069354..2069563 - 210 WP_001058495.1 hypothetical protein Antitoxin
SA_RS10510 2069556..2069702 - 147 WP_000784885.1 hypothetical protein -
SA_RS10515 (SA1832) 2069699..2070016 - 318 WP_000481967.1 helix-turn-helix domain-containing protein -
SA_RS10520 (SA1833) 2070021..2070239 - 219 WP_000153640.1 helix-turn-helix transcriptional regulator -
SA_RS10525 (SA1834) 2070412..2071086 + 675 WP_000620857.1 helix-turn-helix transcriptional regulator -
SA_RS10530 (SA1835) 2071100..2072272 + 1173 WP_000179343.1 site-specific integrase -
SA_RS10535 (SA1836) 2072341..2073957 - 1617 WP_000240642.1 chaperonin GroEL -
SA_RS10540 (SA1837) 2074033..2074317 - 285 WP_000917289.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - scn / chp / sak / see / hlb / sell / sec / tsst-1 / groEL 2006528..2074317 67789


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF1474

(1-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12968.26 Da        Isoelectric Point: 4.3050

>T10116 WP_001078344.1 NC_002745:c2069351-2069025 [Staphylococcus aureus subsp. aureus N315]
MNREIKDLFSDLKLLKDSFEDLKDSNGWHFDELYPYEPNHVLNKDELIGEGFSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFYEIEKASSENFGEESDDAKNSIKVAE

Download         Length: 327 bp

>T10116 NC_002745:c2069351-2069025 [Staphylococcus aureus subsp. aureus N315]
ATGAATCGGGAAATTAAAGATTTATTTAGCGATTTAAAGTTGTTGAAAGATAGTTTTGAGGATTTAAAGGATAGTAATGG
TTGGCATTTTGATGAGCTATATCCATATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTGGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8206.22 Da        Isoelectric Point: 4.3718

>AT10116 WP_001058495.1 NC_002745:c2069563-2069354 [Staphylococcus aureus subsp. aureus N315]
MNNEQKEVIEHVVYQLELSVVNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Download         Length: 210 bp

>AT10116 NC_002745:c2069563-2069354 [Staphylococcus aureus subsp. aureus N315]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCGTGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3K0C1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3JSN0

References

(1) Fuminori Kato et al. (2019) Genome-Wide Screening for Identification of Novel Toxin-Antitoxin Systems in Staphylococcus aureus. Applied And Environmental Microbiology 85(20):e00915-19. [PubMed:31375497] experimental


experimental experimental literature