TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family relE (T1041) Predicted domain PF05016 (ParE)
Locus tag orf2188 Length 95 a.a.
Coordinates 2312060..2312347 (+)

Antitoxin (Protein)


Predicted family relB (AT1041) Predicted domain PRK11235 (RelB)
Locus tag orf2187 Length 79 a.a.
Coordinates 2311821..2312060 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf2182 2307183..2307539 + 357 ORF2182 Predicted ORF by PRODIGAL Version 1.20 -
orf2183 2308692..2308871 + 180 ORF2183 Predicted ORF by PRODIGAL Version 1.20 -
orf2184 2308846..2309028 - 183 ORF2184 Predicted ORF by PRODIGAL Version 1.20 -
orf2185 2309206..2310519 + 1314 ORF2185 Predicted ORF by PRODIGAL Version 1.20 -
orf2186 2310956..2311288 - 333 ORF2186 Predicted ORF by PRODIGAL Version 1.20 -
orf2187 2311821..2312060 + 240 ORF2187 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf2188 2312060..2312347 + 288 ORF2188 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf2189 2312419..2312574 + 156 ORF2189 Predicted ORF by PRODIGAL Version 1.20 -
orf2190 2312791..2313042 + 252 ORF2190 Predicted ORF by PRODIGAL Version 1.20 -
orf2191 2313389..2314438 + 1050 ORF2191 Predicted ORF by PRODIGAL Version 1.20 -
orf2192 2314452..2315204 + 753 ORF2192 Predicted ORF by PRODIGAL Version 1.20 -
orf2193 2315626..2315838 - 213 ORF2193 Predicted ORF by PRODIGAL Version 1.20 -
orf2194 2316139..2316354 + 216 ORF2194 Predicted ORF by PRODIGAL Version 1.20 -
orf2195 2316956..2317075 - 120 ORF2195 Predicted ORF by PRODIGAL Version 1.20 -
orf2196 2317117..2317323 + 207 ORF2196 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

PF05016 (ParE) (E-value: 1.1e-36, Score:123.2)

(1-95)

TIGR02385 (RelE) (E-value: 4.6e-26, Score:89.3)

(1-91)

COG2026 (RelE) (E-value: 2.4e-18, Score:64.2)

(1-87)

ParE-like_toxin (ParE) (E-value: 2.9e-06, Score:25.3)

(6-80)

Antitoxin

PRK11235 (RelB) (E-value: 2.8e-32, Score:108.8)

(1-80)

PF04221 (RelB) (E-value: 2.6e-26, Score:89.6)

(4-84)

TIGR02384 (DinJ) (E-value: 4.8e-24, Score:82.5)

(5-83)

COG3077 (RelB) (E-value: 1.2e-15, Score:55.5)

(5-68)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>ORF2188 TA_17 Toxin
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGY
RLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL

Download         Length: 288 bp

>ORF2188 TA_17 Toxin
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCG
ACGGTACGTGAACAGTTGAAAAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAA
GCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTAAGCTCCGGTCTTCAGGCTAT
CGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>ORF2187 TA_17 Antitoxin
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVE
IVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>ORF2187 TA_17 Antitoxin
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTT
GAAAAAATGGGTGTAACTCCTTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGAC
AATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATGAAGATGCTGAACTTGTGGAG
ATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1041 Escherichia coli str. K-12 substr. MG1655

100

100

1
T173 Vibrio cholerae O1 biovar El Tor str. N16961

56.383

97.917

0.558
T6132 Escherichia coli

54.255

98.947

0.537
T6131 Escherichia coli

54.255

98.947

0.537
T10166 Xenorhabdus nematophila ATCC 19061

58.065

98.936

0.568
T6130 Erwinia pyrifoliae Ep1/96

50

98.947

0.495
T175 Vibrio cholerae O1 biovar El Tor str. N16961

51.064

98.947

0.505
T6023 Xenorhabdus nematophila ATCC 19061

47.059

89.474

0.253
T10157 Streptococcus suis SC84

33.684

100

0.337
T1729 Acidithiobacillus caldus

28.889

100

0.274
T978 Synechocystis sp. PCC 6803

27.66

78.333

0.274
T6285 Streptomyces cattleya NRRL 8057 = DSM 46488

31.325

95.402

0.274
T10158 Streptococcus suis SC84

26.506

96.512

0.232
T727 Streptococcus pneumoniae R6

30.137

86.905

0.232
T326 Streptococcus pneumoniae TIGR4

30.137

86.905

0.232
T174 Vibrio cholerae O1 biovar El Tor str. N16961

23.611

65.455

0.179
Protein Organism Identities (%) Coverage (%) Ha-value
AT1041 Escherichia coli str. K-12 substr. MG1655

100

100

1
AT6023 Xenorhabdus nematophila ATCC 19061

41.667

100

0.443
AT10190 Pseudomonas sp. strain ANT_J3

36.364

50.575

0.203
AT6097 Sinorhizobium meliloti 1021

40.741

52.941

0.278
AT665 Streptococcus mutans UA159

37.778

50

0.215
AT10136 Pseudomonas savastanoi

31.148

55.963

0.241
AT6258 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

38.776

52.128

0.241
AT10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

39.535

47.778

0.215
AT6239 Lactobacillus rhamnosus ATCC 8530

39.535

47.778

0.215
AT6144 Lactobacillus rhamnosus ATCC 8530

39.535

47.778

0.215
AT6139 Lactobacillus rhamnosus Lc 705

39.535

47.778

0.215
AT10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

39.535

47.778

0.215
AT10070 Tetragenococcus halophilus NBRC 12172

27.869

75.309

0.215
AT6290 Escherichia coli BW25113

40

46.512

0.203
AT1038 Escherichia coli str. K-12 substr. MG1655

40

46.512

0.203
AT10181 Aggregatibacter actinomycetemcomitans D11S 1

25

48.889

0.139
AT6261 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

31.507

60.833

0.291
AT6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

27.16

91.011

0.278
AT6260 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

31.818

54.321

0.177
AT10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

31.373

57.303

0.203
AT10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

29.412

57.303

0.19
AT10048 Mycoplasma mycoides subsp. capri str. GM12

25

48.889

0.139
AT166 Vibrio cholerae O1 biovar El Tor str. N16961

25.581

46.739

0.139
AT6289 Bifidobacterium longum subsp. longum JDM301

24.691

91.011

0.253
AT6129 Streptococcus mutans

27.907

45.263

0.152
AT6128 Streptococcus mutans

27.907

45.263

0.152