Detailed information of TA system TA006132

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PHD-RelE
• Location: 2524..3059
• Replicon: plasmid pO111-CRL-115
• Accession: NC_022992
• Organism: Escherichia coli

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T2HS21
• Locus tag: HTA80_RS00015
• Protein ID: WP_000222775.1
• Coordinates: 2772..3059 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T2HSA2
• Locus tag: HTA80_RS00010
• Protein ID: WP_001139207.1
• Coordinates: 2524..2775 (+)
• Length: 84 a.a.

Genomic Context

Location: 703..1575 (873 bp)
Type: Others
Protein ID: WP_000729440.1

Location: 2524..2775 (252 bp)
Type: Antitoxin
Protein ID: WP_001139207.1

Location: 2772..3059 (288 bp)
Type: Toxin
Protein ID: WP_000222775.1

Location: 3069..3614 (546 bp)
Type: Others
Protein ID: WP_000926984.1

Location: 5222..5461 (240 bp)
Type: Others
Protein ID: Protein_5

Location: 4266..4868 (603 bp)
Type: Others
Protein ID: WP_000517694.1

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Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HTA80_RS00005 (D616_p107002)	703..1575	+	873	WP_000729440.1	incFII family plasmid replication initiator RepA	-
HTA80_RS00010 (D616_p107004)	2524..2775	+	252	WP_001139207.1	plasmid stabilization protein	Antitoxin
HTA80_RS00015 (D616_p107005)	2772..3059	+	288	WP_000222775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HTA80_RS00020 (D616_p107006)	3069..3614	+	546	WP_000926984.1	hypothetical protein	-
HTA80_RS00025 (D616_p107008)	4266..4868	-	603	WP_000517694.1	ProQ/FINO family protein	-
HTA80_RS00030 (D616_p107009)	5222..5461	+	240	Protein_5	hypothetical protein	-

Associated MGEs

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MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_022996 Escherichia coli Conjugative plasmid TA006131 1..124908 0.002 View NZ_AP018803 Escherichia coli strain E2863 Conjugative plasmid TA032350 1..124261 0.009 View NZ_AP019762 Escherichia coli O111:H- strain 110512 Conjugative plasmid TA033035 1..118827 0.009 View NZ_CP013024 Escherichia coli strain 2009C-3133 Conjugative plasmid TA057718 1..120611 0.008 View NZ_CP027600 Escherichia coli strain 97-3250 Conjugative plasmid TA099741 1..120604 0.008 View NC_022996 Escherichia coli Conjugative plasmid TA006131 1..124908 0.002	inside	Conjugative plasmid	dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / tet(A)	-	1..115452	115452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.17 Da
• Isoelectric Point: 10.6261

>T6132 WP_000222775.1 NC_022992:2772-3059 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T6132 NC_022992:2772-3059 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9216.45 Da
• Isoelectric Point: 4.0927

>AT6132 WP_001139207.1 NC_022992:2524-2775 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT6132 NC_022992:2524-2775 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

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Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6131	Escherichia coli	100	100	1
T173	Vibrio cholerae O1 biovar El Tor str. N16961	58.511	98.947	0.579
T1041	Escherichia coli str. K-12 substr. MG1655	54.255	98.947	0.537
T175	Vibrio cholerae O1 biovar El Tor str. N16961	54.255	98.947	0.537
T10166	Xenorhabdus nematophila ATCC 19061	52.688	98.936	0.521
T6130	Erwinia pyrifoliae Ep1/96	46.809	98.947	0.463
T6023	Xenorhabdus nematophila ATCC 19061	45.098	89.474	0.404

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Multiple sequence alignment

Antitoxin

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Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6131	Escherichia coli	100	100	1
AT173	Vibrio cholerae O1 biovar El Tor str. N16961	47.5	97.561	0.463
AT10166	Xenorhabdus nematophila ATCC 19061	56.923	78.313	0.446
AT175	Vibrio cholerae O1 biovar El Tor str. N16961	52.308	78.313	0.41
AT6130	Erwinia pyrifoliae Ep1/96	48.529	81.928	0.398
AT6157	Escherichia coli str. K-12 substr. MG1655	30.435	100	0.337

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Multiple sequence alignment

Structures

Toxin

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• AlphaFold DB: T2HS21

Antitoxin

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• AlphaFold DB: A0A754EQ65

References

(1) Carola Venturini et al. (2013) Sequences of two related multiple antibiotic resistance virulence plasmids sharing a unique IS26-related molecular signature isolated from different Escherichia coli pathotypes from different hosts. PloS One 8(11):e78862. [PubMed:24223859]