Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/-PHD |
| Location | 252005..252699 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | b0233 | Protein ID | NP_414768.1 |
| Coordinates | 252301..252699 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | Q47156 |
| Locus tag | b0232 | Protein ID | NP_414767.1 |
| Coordinates | 252005..252298 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b0228 | 247637..248134 | + | 498 | NP_414763.1 | REP-associated tyrosine transposase | - |
| b0231 | 250898..251953 | + | 1056 | NP_414766.1 | DNA polymerase IV | - |
| b0232 | 252005..252298 | + | 294 | NP_414767.1 | antitoxin YafN | Antitoxin |
| b0233 | 252301..252699 | + | 399 | NP_414768.1 | ribosome-dependent mRNA interferase toxin YafO | Toxin |
| b0234 | 252709..253161 | + | 453 | NP_414769.1 | putative N-acetyltransferase YafP | - |
| b0237 | 254259..255716 | - | 1458 | NP_414772.1 | peptidase D | - |
| b0238 | 255977..256435 | + | 459 | NP_414773.1 | xanthine-guanine phsophoribosyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T6157 NP_414768.1 NC_000913:252301-252699 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T6157 NC_000913:252301-252699 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT6157 NP_414767.1 NC_000913:252005-252298 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT6157 NC_000913:252005-252298 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6132 | Escherichia coli |
30.435 |
100 |
0.337 |
| AT6131 | Escherichia coli |
30.435 |
100 |
0.337 |
| AT173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
44.828 |
70.732 |
0.317 |
| AT10064 | Dickeya dadantii 3937 |
34.247 |
92.405 |
0.316 |
| AT175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
36.765 |
81.928 |
0.301 |
Multiple sequence alignment
References
(1) Fan Zhang et al. (2012) Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli. Acta Crystallographica. Section F, Structural Biology And Crystallization Communications 68(Pt 8):894-7. [PubMed:22869116]
(2) Mikkel Christensen-Dalsgaard et al. (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Molecular Microbiology 75(2):333-48. [PubMed:19943910]
(3) Larissa A Singletary et al. (2009) An SOS-regulated type 2 toxin-antitoxin system. Journal of Bacteriology 191(24):7456-65. [PubMed:19837801]