Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/PHD-RelE |
| Location | 2524..3059 | Replicon | plasmid pO26-CRL-125 |
| Accession | NC_022996 | ||
| Organism | Escherichia coli | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | T2HS21 |
| Locus tag | HTC40_RS00015 | Protein ID | WP_000222775.1 |
| Coordinates | 2772..3059 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | T2HSA2 |
| Locus tag | HTC40_RS00010 | Protein ID | WP_001139207.1 |
| Coordinates | 2524..2775 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTC40_RS00005 (D616_p108002) | 703..1575 | + | 873 | WP_000729440.1 | incFII family plasmid replication initiator RepA | - |
| HTC40_RS00010 (D616_p108004) | 2524..2775 | + | 252 | WP_001139207.1 | plasmid stabilization protein | Antitoxin |
| HTC40_RS00015 (D616_p108005) | 2772..3059 | + | 288 | WP_000222775.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HTC40_RS00020 (D616_p108006) | 3069..3614 | + | 546 | WP_000926984.1 | hypothetical protein | - |
| HTC40_RS00025 (D616_p108008) | 4266..4868 | - | 603 | WP_000517694.1 | ProQ/FINO family protein | - |
| HTC40_RS00030 (D616_p108009) | 5222..5461 | + | 240 | Protein_5 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Conjugative plasmid | dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / tet(A) | - | 1..124908 | 124908 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11142.17 Da Isoelectric Point: 10.6261
>T6131 WP_000222775.1 NC_022996:2772-3059 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR
Download Length: 288 bp
>T6131 NC_022996:2772-3059 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9216.45 Da Isoelectric Point: 4.0927
>AT6131 WP_001139207.1 NC_022996:2524-2775 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT6131 NC_022996:2524-2775 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6132 | Escherichia coli |
100 |
100 |
1 |
| T173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
58.511 |
98.947 |
0.579 |
| T175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
54.255 |
98.947 |
0.537 |
| T1041 | Escherichia coli str. K-12 substr. MG1655 |
54.255 |
98.947 |
0.537 |
| T10166 | Xenorhabdus nematophila ATCC 19061 |
52.688 |
98.936 |
0.521 |
| T6130 | Erwinia pyrifoliae Ep1/96 |
46.809 |
98.947 |
0.463 |
| T6023 | Xenorhabdus nematophila ATCC 19061 |
45.098 |
89.474 |
0.404 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6132 | Escherichia coli |
100 |
100 |
1 |
| AT173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
47.5 |
97.561 |
0.463 |
| AT10166 | Xenorhabdus nematophila ATCC 19061 |
56.923 |
78.313 |
0.446 |
| AT175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
52.308 |
78.313 |
0.41 |
| AT6130 | Erwinia pyrifoliae Ep1/96 |
48.529 |
81.928 |
0.398 |
| AT6157 | Escherichia coli str. K-12 substr. MG1655 |
30.435 |
100 |
0.337 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | T2HS21 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A754EQ65 |
References
(1) Carola Venturini et al. (2013) Sequences of two related multiple antibiotic resistance virulence plasmids sharing a unique IS26-related molecular signature isolated from different Escherichia coli pathotypes from different hosts. PloS One 8(11):e78862. [PubMed:24223859]