Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 2939582..2940107 | Replicon | chromosome |
| Accession | NC_014228 | ||
| Organism | Xenorhabdus nematophila ATCC 19061 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | N1NPN2 |
| Locus tag | XNC1_RS12315 | Protein ID | WP_010848883.1 |
| Coordinates | 2939582..2939866 (-) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | N1NTS9 |
| Locus tag | XNC1_RS12320 | Protein ID | WP_010848882.1 |
| Coordinates | 2939856..2940107 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| XNC1_RS12295 (XNC1_2940) | 2935061..2935993 | + | 933 | Protein_2444 | IS66 family transposase | - |
| XNC1_RS21545 | 2936135..2937256 | + | 1122 | Protein_2445 | ISAs1 family transposase | - |
| XNC1_RS12305 (XNC1_2944) | 2937618..2938154 | + | 537 | WP_013184731.1 | gluconokinase | - |
| XNC1_RS12310 (XNC1_2945) | 2938154..2939491 | + | 1338 | WP_010848884.1 | gluconate transporter | - |
| XNC1_RS12315 (XNC1_2946) | 2939582..2939866 | - | 285 | WP_010848883.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| XNC1_RS12320 (XNC1_2947) | 2939856..2940107 | - | 252 | WP_010848882.1 | plasmid stabilization protein | Antitoxin |
| XNC1_RS23760 (XNC1_2948) | 2940188..2940514 | - | 327 | WP_050986632.1 | hypothetical protein | - |
| XNC1_RS12330 (XNC1_2950) | 2940821..2941909 | - | 1089 | WP_013184732.1 | DUF459 domain-containing protein | - |
| XNC1_RS12335 (XNC1_2951) | 2941896..2943313 | - | 1418 | Protein_2452 | MBOAT family protein | - |
| XNC1_RS12340 (XNC1_2954) | 2943506..2944282 | - | 777 | WP_010848877.1 | protein bax | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10934.63 Da Isoelectric Point: 10.2279
>T10166 WP_010848883.1 NC_014228:c2939866-2939582 [Xenorhabdus nematophila ATCC 19061]
MTYSLKFEKRALKEWEKLGHPIREQFKKKLSERLENPHISSARLSGRNNLYKIKLCSSGYRLVYEVNDHAIVLLVIAVGK
RSGNQVYSVADERE
MTYSLKFEKRALKEWEKLGHPIREQFKKKLSERLENPHISSARLSGRNNLYKIKLCSSGYRLVYEVNDHAIVLLVIAVGK
RSGNQVYSVADERE
Download Length: 285 bp
>T10166 NC_014228:c2939866-2939582 [Xenorhabdus nematophila ATCC 19061]
ATGACTTATAGTCTCAAATTTGAAAAGCGGGCACTAAAAGAGTGGGAAAAACTAGGGCATCCTATCCGTGAGCAGTTCAA
AAAGAAATTGTCTGAGCGTTTAGAAAACCCCCATATATCTTCAGCTCGATTGAGTGGCCGCAATAATCTCTACAAAATCA
AATTATGTTCTTCTGGCTATCGTTTAGTCTACGAAGTCAATGATCATGCTATTGTTTTACTGGTGATCGCTGTTGGTAAA
AGATCTGGAAATCAGGTTTATTCAGTCGCTGATGAACGTGAATAA
ATGACTTATAGTCTCAAATTTGAAAAGCGGGCACTAAAAGAGTGGGAAAAACTAGGGCATCCTATCCGTGAGCAGTTCAA
AAAGAAATTGTCTGAGCGTTTAGAAAACCCCCATATATCTTCAGCTCGATTGAGTGGCCGCAATAATCTCTACAAAATCA
AATTATGTTCTTCTGGCTATCGTTTAGTCTACGAAGTCAATGATCATGCTATTGTTTTACTGGTGATCGCTGTTGGTAAA
AGATCTGGAAATCAGGTTTATTCAGTCGCTGATGAACGTGAATAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9140.29 Da Isoelectric Point: 3.9282
>AT10166 WP_010848882.1 NC_014228:c2940107-2939856 [Xenorhabdus nematophila ATCC 19061]
MSYQILTTIAASITDLKRNPMGTVADGEGGAVAILNRNEPAFYCVPPELYAYYLELAEDAELNHIADERMKSAEFVSVDL
NDL
MSYQILTTIAASITDLKRNPMGTVADGEGGAVAILNRNEPAFYCVPPELYAYYLELAEDAELNHIADERMKSAEFVSVDL
NDL
Download Length: 252 bp
>AT10166 NC_014228:c2940107-2939856 [Xenorhabdus nematophila ATCC 19061]
ATGTCTTATCAGATCCTGACAACAATAGCAGCCAGCATTACCGATTTGAAACGCAATCCCATGGGAACCGTCGCTGATGG
TGAAGGTGGTGCTGTCGCTATCCTGAACCGTAATGAACCTGCATTCTATTGTGTTCCACCCGAGCTTTACGCTTACTATT
TAGAACTTGCAGAGGATGCAGAATTAAATCACATCGCAGATGAACGGATGAAATCCGCTGAATTTGTGAGCGTCGATCTC
AATGACTTATAG
ATGTCTTATCAGATCCTGACAACAATAGCAGCCAGCATTACCGATTTGAAACGCAATCCCATGGGAACCGTCGCTGATGG
TGAAGGTGGTGCTGTCGCTATCCTGAACCGTAATGAACCTGCATTCTATTGTGTTCCACCCGAGCTTTACGCTTACTATT
TAGAACTTGCAGAGGATGCAGAATTAAATCACATCGCAGATGAACGGATGAAATCCGCTGAATTTGTGAGCGTCGATCTC
AATGACTTATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
62.366 |
98.936 |
0.617 |
| T6130 | Erwinia pyrifoliae Ep1/96 |
62.366 |
98.936 |
0.617 |
| T1041 | Escherichia coli str. K-12 substr. MG1655 |
58.065 |
98.936 |
0.574 |
| T175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
60.92 |
92.553 |
0.564 |
| T6132 | Escherichia coli |
52.688 |
98.936 |
0.521 |
| T6131 | Escherichia coli |
52.688 |
98.936 |
0.521 |
| T6023 | Xenorhabdus nematophila ATCC 19061 |
54.348 |
80.702 |
0.439 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6130 | Erwinia pyrifoliae Ep1/96 |
63.095 |
100 |
0.639 |
| AT173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
50.602 |
100 |
0.512 |
| AT6132 | Escherichia coli |
56.923 |
78.313 |
0.446 |
| AT6131 | Escherichia coli |
56.923 |
78.313 |
0.446 |
| AT175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
43.21 |
97.59 |
0.422 |
Multiple sequence alignment
References
(1) Mohit Yadav et al. (2018) TAome analysis of type-II toxin-antitoxin system from Xenorhabdus nematophila. Computational Biology And Chemistry 76:293-301. [PubMed:30114602]