Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | stbDE/- |
| Location | 12792..13323 | Replicon | plasmid pEP36 |
| Accession | NC_004445 | ||
| Organism | Erwinia pyrifoliae Ep1/96 | ||
Toxin (Protein)
| Gene name | stbE | Uniprot ID | E3DL65 |
| Locus tag | HS200_RS00060 | Protein ID | WP_011078058.1 |
| Coordinates | 12792..13079 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | stbD | Uniprot ID | Q7X3U3 |
| Locus tag | HS200_RS00065 | Protein ID | WP_011078059.1 |
| Coordinates | 13069..13323 (-) | Length | 85 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HS200_RS00040 (pEP36_010) | 9293..10849 | - | 1557 | WP_173479934.1 | methyl-accepting chemotaxis protein | - |
| HS200_RS00045 | 11357..11769 | - | 413 | Protein_8 | transcriptional regulator | - |
| HS200_RS00050 | 11770..12084 | - | 315 | WP_041473956.1 | type II toxin-antitoxin system HigB family toxin | - |
| HS200_RS00055 (pEP36_011) | 12348..12632 | - | 285 | WP_011078079.1 | VF530 family DNA-binding protein | - |
| HS200_RS00060 (pEP36_012) | 12792..13079 | - | 288 | WP_011078058.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HS200_RS00065 (pEP36_013) | 13069..13323 | - | 255 | WP_011078059.1 | plasmid stabilization protein | Antitoxin |
| HS200_RS00070 (pEP36_014) | 13534..14499 | - | 966 | WP_011078060.1 | ThiF family adenylyltransferase | - |
| HS200_RS00075 (pEP36_015) | 14515..15273 | - | 759 | WP_173479937.1 | thiazole synthase | - |
| HS200_RS00080 | 15277..15474 | - | 198 | WP_012814637.1 | sulfur carrier protein ThiS | - |
| HS200_RS00085 (pEP36_016) | 15471..16403 | - | 933 | WP_041473952.1 | FAD-dependent oxidoreductase | - |
| HS200_RS00090 | 16779..16940 | - | 162 | Protein_17 | Txe/YoeB family addiction module toxin | - |
| HS200_RS00095 (pEP36_017) | 17120..17704 | + | 585 | WP_011078063.1 | recombinase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| flank | IS/Tn | - | - | 17120..17704 | 584 | ||
| inside | Non-Mobilizable plasmid | - | pla | 1..35904 | 35904 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11197.09 Da Isoelectric Point: 10.6516
>T6130 WP_011078058.1 NC_004445:c13079-12792 [Erwinia pyrifoliae Ep1/96]
MTYKLKFVPSAEKEWKRLGHPVREQFKKKLAERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQEHTITIMVMGIGK
REGNQIYSDTENRLP
MTYKLKFVPSAEKEWKRLGHPVREQFKKKLAERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQEHTITIMVMGIGK
REGNQIYSDTENRLP
Download Length: 288 bp
>T6130 NC_004445:c13079-12792 [Erwinia pyrifoliae Ep1/96]
ATGACTTATAAACTCAAGTTTGTCCCTTCCGCAGAGAAGGAATGGAAAAGGCTCGGGCACCCTGTCAGGGAGCAGTTCAA
AAAAAAGCTTGCTGAACGCCTGGAAAACCCCCGAGTGCCTTCAGCACAGCTTCATGGTCGTAAAGATCAGTACAAAATTA
AGCTAAAGTCTGCCGGTTACAGGCTGGTTTATCTGGTCCAGGAGCACACCATTACGATAATGGTTATGGGCATTGGCAAA
CGTGAAGGTAATCAGATTTACTCTGATACAGAGAACCGGCTTCCCTGA
ATGACTTATAAACTCAAGTTTGTCCCTTCCGCAGAGAAGGAATGGAAAAGGCTCGGGCACCCTGTCAGGGAGCAGTTCAA
AAAAAAGCTTGCTGAACGCCTGGAAAACCCCCGAGTGCCTTCAGCACAGCTTCATGGTCGTAAAGATCAGTACAAAATTA
AGCTAAAGTCTGCCGGTTACAGGCTGGTTTATCTGGTCCAGGAGCACACCATTACGATAATGGTTATGGGCATTGGCAAA
CGTGAAGGTAATCAGATTTACTCTGATACAGAGAACCGGCTTCCCTGA
Antitoxin
Download Length: 85 a.a. Molecular weight: 9210.56 Da Isoelectric Point: 3.8705
>AT6130 WP_011078059.1 NC_004445:c13323-13069 [Erwinia pyrifoliae Ep1/96]
MAFNILTNTAASITDLKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMYAYLMELAEDAELGCIVDDRMSELPVAKEIS
LDDL
MAFNILTNTAASITDLKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMYAYLMELAEDAELGCIVDDRMSELPVAKEIS
LDDL
Download Length: 255 bp
>AT6130 NC_004445:c13323-13069 [Erwinia pyrifoliae Ep1/96]
ATGGCATTCAACATACTCACTAATACCGCAGCAAGCATCACGGATTTGAAGCGCGACCCAATGGGCACAGTCCGTGCTGC
CGAAGGGGAAACTATCGCAATTTTAAACCGTAACGAGCCGGCATTTTATTGCGTACCACCCGCTATGTACGCTTATCTGA
TGGAGCTGGCAGAGGATGCTGAGCTTGGATGTATCGTTGACGATCGCATGTCAGAGTTACCGGTTGCGAAAGAGATATCA
TTGGATGACTTATAA
ATGGCATTCAACATACTCACTAATACCGCAGCAAGCATCACGGATTTGAAGCGCGACCCAATGGGCACAGTCCGTGCTGC
CGAAGGGGAAACTATCGCAATTTTAAACCGTAACGAGCCGGCATTTTATTGCGTACCACCCGCTATGTACGCTTATCTGA
TGGAGCTGGCAGAGGATGCTGAGCTTGGATGTATCGTTGACGATCGCATGTCAGAGTTACCGGTTGCGAAAGAGATATCA
TTGGATGACTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10166 | Xenorhabdus nematophila ATCC 19061 |
62.366 |
98.936 |
0.617 |
| T173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
56.383 |
98.947 |
0.558 |
| T175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
53.191 |
98.947 |
0.526 |
| T1041 | Escherichia coli str. K-12 substr. MG1655 |
50 |
98.947 |
0.495 |
| T6132 | Escherichia coli |
46.809 |
98.947 |
0.463 |
| T6131 | Escherichia coli |
46.809 |
98.947 |
0.463 |
| T6023 | Xenorhabdus nematophila ATCC 19061 |
45.652 |
80.702 |
0.368 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10166 | Xenorhabdus nematophila ATCC 19061 |
63.095 |
100 |
0.639 |
| AT173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
47.619 |
100 |
0.488 |
| AT175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
46.25 |
96.386 |
0.446 |
| AT6132 | Escherichia coli |
48.529 |
81.928 |
0.398 |
| AT6131 | Escherichia coli |
48.529 |
81.928 |
0.398 |
Multiple sequence alignment
References
(1) Simon J Unterholzner et al. (2013) Characterisation of the stbD/E toxin-antitoxin system of pEP36, a plasmid of the plant pathogen Erwinia pyrifoliae. Plasmid 70(2):216-25. [PubMed:23632277]