Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) stbDE/-
Location 12792..13323 Replicon plasmid pEP36
Accession NC_004445
Organism Erwinia pyrifoliae Ep1/96

Toxin (Protein)


Gene name stbE Uniprot ID E3DL65
Locus tag HS200_RS00060 Protein ID WP_011078058.1
Coordinates 12792..13079 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID Q7X3U3
Locus tag HS200_RS00065 Protein ID WP_011078059.1
Coordinates 13069..13323 (-) Length 85 a.a.

Genomic Context

HS200_RS00095HS200_RS00040HS200_RS00045HS200_RS00050HS200_RS00055HS200_RS00060HS200_RS00065HS200_RS00070HS200_RS00075HS200_RS00080HS200_RS00085HS200_RS00090
Location: 17120..17704 (585 bp)
Type: Others
Protein ID: WP_011078063.1
Location: 9293..10849 (1557 bp)
Type: Others
Protein ID: WP_173479934.1
Location: 11357..11769 (413 bp)
Type: Others
Protein ID: Protein_8
Location: 11770..12084 (315 bp)
Type: Others
Protein ID: WP_041473956.1
Location: 12348..12632 (285 bp)
Type: Others
Protein ID: WP_011078079.1
Location: 12792..13079 (288 bp)
Type: Toxin
Protein ID: WP_011078058.1
Location: 13069..13323 (255 bp)
Type: Antitoxin
Protein ID: WP_011078059.1
Location: 13534..14499 (966 bp)
Type: Others
Protein ID: WP_011078060.1
Location: 14515..15273 (759 bp)
Type: Others
Protein ID: WP_173479937.1
Location: 15277..15474 (198 bp)
Type: Others
Protein ID: WP_012814637.1
Location: 15471..16403 (933 bp)
Type: Others
Protein ID: WP_041473952.1
Location: 16779..16940 (162 bp)
Type: Others
Protein ID: Protein_17
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
HS200_RS00040 (pEP36_010)9293..10849-1557WP_173479934.1methyl-accepting chemotaxis protein-
HS200_RS0004511357..11769-413Protein_8transcriptional regulator-
HS200_RS0005011770..12084-315WP_041473956.1type II toxin-antitoxin system HigB family toxin-
HS200_RS00055 (pEP36_011)12348..12632-285WP_011078079.1VF530 family DNA-binding protein-
HS200_RS00060 (pEP36_012)12792..13079-288WP_011078058.1type II toxin-antitoxin system RelE/ParE family toxinToxin
HS200_RS00065 (pEP36_013)13069..13323-255WP_011078059.1plasmid stabilization proteinAntitoxin
HS200_RS00070 (pEP36_014)13534..14499-966WP_011078060.1ThiF family adenylyltransferase-
HS200_RS00075 (pEP36_015)14515..15273-759WP_173479937.1thiazole synthase-
HS200_RS0008015277..15474-198WP_012814637.1sulfur carrier protein ThiS-
HS200_RS00085 (pEP36_016)15471..16403-933WP_041473952.1FAD-dependent oxidoreductase-
HS200_RS0009016779..16940-162Protein_17Txe/YoeB family addiction module toxin-
HS200_RS00095 (pEP36_017)17120..17704+585WP_011078063.1recombinase family protein-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
flankIS/Tn--17120..17704584
insideNon-Mobilizable plasmid-pla1..3590435904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11197.09 Da        Isoelectric Point: 10.6516

>T6130 WP_011078058.1 NC_004445:c13079-12792 [Erwinia pyrifoliae Ep1/96]
MTYKLKFVPSAEKEWKRLGHPVREQFKKKLAERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQEHTITIMVMGIGK
REGNQIYSDTENRLP

Download         Length: 288 bp

>T6130 NC_004445:c13079-12792 [Erwinia pyrifoliae Ep1/96]
ATGACTTATAAACTCAAGTTTGTCCCTTCCGCAGAGAAGGAATGGAAAAGGCTCGGGCACCCTGTCAGGGAGCAGTTCAA
AAAAAAGCTTGCTGAACGCCTGGAAAACCCCCGAGTGCCTTCAGCACAGCTTCATGGTCGTAAAGATCAGTACAAAATTA
AGCTAAAGTCTGCCGGTTACAGGCTGGTTTATCTGGTCCAGGAGCACACCATTACGATAATGGTTATGGGCATTGGCAAA
CGTGAAGGTAATCAGATTTACTCTGATACAGAGAACCGGCTTCCCTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9210.56 Da        Isoelectric Point: 3.8705

>AT6130 WP_011078059.1 NC_004445:c13323-13069 [Erwinia pyrifoliae Ep1/96]
MAFNILTNTAASITDLKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMYAYLMELAEDAELGCIVDDRMSELPVAKEIS
LDDL

Download         Length: 255 bp

>AT6130 NC_004445:c13323-13069 [Erwinia pyrifoliae Ep1/96]
ATGGCATTCAACATACTCACTAATACCGCAGCAAGCATCACGGATTTGAAGCGCGACCCAATGGGCACAGTCCGTGCTGC
CGAAGGGGAAACTATCGCAATTTTAAACCGTAACGAGCCGGCATTTTATTGCGTACCACCCGCTATGTACGCTTATCTGA
TGGAGCTGGCAGAGGATGCTGAGCTTGGATGTATCGTTGACGATCGCATGTCAGAGTTACCGGTTGCGAAAGAGATATCA
TTGGATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T10166Xenorhabdus nematophila ATCC 19061

62.366

98.936

0.617

T173Vibrio cholerae O1 biovar El Tor str. N16961

56.383

98.947

0.558

T175Vibrio cholerae O1 biovar El Tor str. N16961

53.191

98.947

0.526

T1041Escherichia coli str. K-12 substr. MG1655

50

98.947

0.495

T6132Escherichia coli

46.809

98.947

0.463

T6131Escherichia coli

46.809

98.947

0.463

T6023Xenorhabdus nematophila ATCC 19061

45.652

80.702

0.368
Showing 1 to 7 of 7 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT10166Xenorhabdus nematophila ATCC 19061

63.095

100

0.639

AT173Vibrio cholerae O1 biovar El Tor str. N16961

47.619

100

0.488

AT175Vibrio cholerae O1 biovar El Tor str. N16961

46.25

96.386

0.446

AT6132Escherichia coli

48.529

81.928

0.398

AT6131Escherichia coli

48.529

81.928

0.398
Showing 1 to 5 of 5 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
AlphaFold DBE3DL65

Antitoxin

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Source
ID
Structure
AlphaFold DBQ7X3U3

References

(1) Simon J Unterholzner et al. (2013) Characterisation of the stbD/E toxin-antitoxin system of pEP36, a plasmid of the plant pathogen Erwinia pyrifoliae. Plasmid 70(2):216-25. [PubMed:23632277] experimental


experimental experimental literature