Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 17633..18361 Replicon plasmid pEP36
Accession NC_013263
Organism Erwinia pyrifoliae Ep1/96

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EPC_RS00135 Protein ID WP_041473956.1
Coordinates 17633..17947 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EPC_RS00140 Protein ID WP_011113999.1
Coordinates 17948..18361 (+) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPC_RS18745 12747..12938 + 192 Protein_10 Txe/YoeB family addiction module toxin -
EPC_RS00100 13314..14246 + 933 WP_041473952.1 FAD-dependent oxidoreductase -
EPC_RS00105 14243..14440 + 198 WP_012814637.1 sulfur carrier protein ThiS -
EPC_RS00110 14444..15202 + 759 WP_012814638.1 thiazole synthase -
EPC_RS00115 15218..16183 + 966 WP_011078060.1 ThiF family adenylyltransferase -
EPC_RS00120 16394..16648 + 255 WP_011078059.1 plasmid stabilization protein -
EPC_RS00125 16638..16925 + 288 WP_011078058.1 type II toxin-antitoxin system RelE/ParE family toxin -
EPC_RS00130 17085..17369 + 285 WP_011078079.1 VF530 family protein -
EPC_RS00135 17633..17947 + 315 WP_041473956.1 type II toxin-antitoxin system HigB family toxin Toxin
EPC_RS00140 17948..18361 + 414 WP_011113999.1 helix-turn-helix domain-containing protein Antitoxin
EPC_RS00145 18869..20425 + 1557 WP_041473953.1 MCP four helix bundle domain-containing protein -
EPC_RS00150 20725..22113 - 1389 WP_041473957.1 NAD-dependent succinate-semialdehyde dehydrogenase -
EPC_RS00155 22254..23111 + 858 WP_012814642.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - pla 1..35909 35909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12227.32 Da        Isoelectric Point: 10.2275

>T24261 WP_041473956.1 NC_013263:17633-17947 [Erwinia pyrifoliae Ep1/96]
MHLISRSMIKDATDKYPQHSRELLALMIAIEKGYFKAPEALKAAFPSLDNLKYLDKHYVINIGRNDLRLVALVFFESQKF
YVRHVFTHKEYDVFTALHRTKGKK

Download         Length: 315 bp

>T24261 NC_013263:17633-17947 [Erwinia pyrifoliae Ep1/96]
ATGCACTTGATAAGCCGTAGCATGATTAAGGATGCCACTGATAAGTACCCGCAGCATTCCAGGGAACTCCTGGCTCTGAT
GATCGCTATTGAAAAAGGGTACTTTAAGGCTCCCGAAGCGTTAAAGGCGGCGTTTCCATCACTGGACAATCTGAAATATT
TAGATAAGCACTACGTTATCAATATTGGCCGCAATGACTTGCGTTTAGTTGCGCTGGTATTTTTTGAGTCTCAAAAATTT
TATGTAAGACATGTGTTTACCCACAAAGAGTACGACGTATTTACAGCGTTACATCGCACAAAGGGGAAAAAATAA

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 14905.32 Da        Isoelectric Point: 4.6233

>AT24261 WP_011113999.1 NC_013263:17948-18361 [Erwinia pyrifoliae Ep1/96]
MLVADIIKAGEALTAVAPFLAGITDKRQHAEALEFVEHLLLNDPENPLLDLVCAKITAYEDTAPEFAEFNAQLAAMPAGV
AMIRLLLDQHDLTLSDLPEIGSKSMVSRVLNGERKLTLEHAKKLATRFAISPALFID

Download         Length: 414 bp

>AT24261 NC_013263:17948-18361 [Erwinia pyrifoliae Ep1/96]
ATGCTCGTAGCCGACATCATCAAAGCCGGAGAGGCTTTAACCGCCGTTGCCCCTTTCCTTGCGGGTATTACTGACAAACG
GCAACATGCAGAGGCGCTGGAATTTGTGGAGCACCTTTTACTTAACGACCCGGAAAACCCACTTTTAGATCTGGTTTGTG
CCAAAATCACGGCTTACGAAGACACAGCGCCCGAATTCGCAGAATTTAATGCACAGCTGGCAGCCATGCCGGCAGGCGTT
GCTATGATCCGTTTGCTGCTCGATCAGCACGATTTAACGTTATCCGATTTACCCGAAATCGGTAGCAAATCGATGGTTTC
CCGCGTACTCAATGGCGAGCGTAAACTGACCCTGGAACATGCCAAAAAGTTAGCAACGCGGTTTGCCATATCTCCGGCGT
TGTTCATCGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References