81645

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 10857..11388 Replicon plasmid pEP48
Accession NZ_CP023568
Organism Erwinia pyrifoliae strain EpK1/15

Toxin (Protein)


Gene name stbE Uniprot ID E3DL65
Locus tag CPI84_RS19345 Protein ID WP_011078058.1
Coordinates 10857..11144 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID Q7X3U3
Locus tag CPI84_RS19350 Protein ID WP_011078059.1
Coordinates 11134..11388 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPI84_RS19325 7357..8913 - 1557 WP_041473953.1 MCP four helix bundle domain-containing protein -
CPI84_RS19330 9421..9834 - 414 WP_011113999.1 helix-turn-helix domain-containing protein -
CPI84_RS19335 9835..10149 - 315 WP_041473956.1 type II toxin-antitoxin system HigB family toxin -
CPI84_RS19340 10413..10697 - 285 WP_011078079.1 VF530 family protein -
CPI84_RS19345 10857..11144 - 288 WP_011078058.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPI84_RS19350 11134..11388 - 255 WP_011078059.1 plasmid stabilization protein Antitoxin
CPI84_RS19355 11599..12564 - 966 WP_011078060.1 ThiF family adenylyltransferase -
CPI84_RS19360 12580..13338 - 759 WP_012814638.1 thiazole synthase -
CPI84_RS19365 13342..13539 - 198 WP_012814637.1 sulfur carrier protein ThiS -
CPI84_RS19370 13536..14468 - 933 WP_041473952.1 FAD-dependent oxidoreductase -
CPI84_RS19375 14844..15035 - 192 Protein_15 Txe/YoeB family addiction module toxin -
CPI84_RS19380 15185..15769 + 585 WP_012814636.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - pla / pla 1..48456 48456
flank IS/Tn - - 15185..15769 584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11197.09 Da        Isoelectric Point: 10.6516

>T81645 WP_011078058.1 NZ_CP023568:c11144-10857 [Erwinia pyrifoliae]
MTYKLKFVPSAEKEWKRLGHPVREQFKKKLAERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQEHTITIMVMGIGK
REGNQIYSDTENRLP

Download         Length: 288 bp

>T81645 NZ_CP023568:c11144-10857 [Erwinia pyrifoliae]
ATGACTTATAAACTCAAGTTTGTCCCTTCCGCAGAGAAGGAATGGAAAAGGCTCGGGCACCCTGTCAGGGAGCAGTTCAA
AAAAAAGCTTGCTGAACGCCTGGAAAACCCCCGAGTGCCTTCAGCACAGCTTCATGGTCGTAAAGATCAGTACAAAATTA
AGCTAAAGTCTGCCGGTTACAGGCTGGTTTATCTGGTCCAGGAGCACACCATTACGATAATGGTTATGGGCATTGGCAAA
CGTGAAGGTAATCAGATTTACTCTGATACAGAGAACCGGCTTCCCTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9210.56 Da        Isoelectric Point: 3.8705

>AT81645 WP_011078059.1 NZ_CP023568:c11388-11134 [Erwinia pyrifoliae]
MAFNILTNTAASITDLKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMYAYLMELAEDAELGCIVDDRMSELPVAKEIS
LDDL

Download         Length: 255 bp

>AT81645 NZ_CP023568:c11388-11134 [Erwinia pyrifoliae]
ATGGCATTCAACATACTCACTAATACCGCAGCAAGCATCACGGATTTGAAGCGCGACCCAATGGGCACAGTCCGTGCTGC
CGAAGGGGAAACTATCGCAATTTTAAACCGTAACGAGCCGGCATTTTATTGCGTACCACCCGCTATGTACGCTTATCTGA
TGGAGCTGGCAGAGGATGCTGAGCTTGGATGTATCGTTGACGATCGCATGTCAGAGTTACCGGTTGCGAAAGAGATATCA
TTGGATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E3DL65

Antitoxin

Source ID Structure
AlphaFold DB Q7X3U3

References