Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 1844823..1845240 | Replicon | chromosome |
| Accession | NC_014228 | ||
| Organism | Xenorhabdus nematophila ATCC 19061 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | XNC1_1940 | Protein ID | WP_013184145.1 |
| Coordinates | 1844823..1844996 (-) | Length | 58 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | N1NP74 |
| Locus tag | XNC1_1941 | Protein ID | WP_010847387.1 |
| Coordinates | 1844986..1845240 (-) | Length | 85 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| XNC1_RS08225 (XNC1_1936) | 1840164..1840748 | + | 585 | WP_143767639.1 | manganese efflux pump MntP | - |
| XNC1_RS08230 (XNC1_1937) | 1840858..1841679 | - | 822 | WP_041573672.1 | 23S rRNA (guanine(745)-N(1))-methyltransferase | - |
| XNC1_RS08235 (XNC1_1938) | 1841963..1843291 | - | 1329 | WP_010847384.1 | MFS transporter | - |
| XNC1_RS08240 (XNC1_1939) | 1843300..1844823 | - | 1524 | WP_013184144.1 | signal transduction histidine-protein kinase/phosphatase UhpB | - |
| XNC1_RS08245 (XNC1_1940) | 1844823..1844996 | - | 174 | WP_013184145.1 | toxin-antitoxin system protein | Toxin |
| XNC1_RS08250 (XNC1_1941) | 1844986..1845240 | - | 255 | WP_010847387.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| XNC1_RS08255 (XNC1_1943) | 1845680..1846060 | + | 381 | WP_113967564.1 | transposase | - |
| XNC1_RS08260 (XNC1_1944) | 1846066..1846683 | - | 618 | WP_010847389.1 | transcriptional regulator UhpA | - |
| XNC1_RS08265 (XNC1_1945) | 1846825..1848201 | - | 1377 | WP_010847390.1 | hexose-6-phosphate:phosphate antiporter | - |
| XNC1_RS08270 (XNC1_1947) | 1848808..1849143 | - | 336 | WP_013184149.1 | hypothetical protein | - |
| XNC1_RS08275 (XNC1_1948) | 1849529..1850218 | + | 690 | WP_010847392.1 | helix-turn-helix transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 58 a.a. Molecular weight: 6973.13 Da Isoelectric Point: 10.1541
>T6023 WP_013184145.1 NC_014228:c1844996-1844823 [Xenorhabdus nematophila ATCC 19061]
MIFNIEFDERAVKEWQKLDNSIREQFKKKLKKLQKNPYIEPARLHGDLACFLRNFSF
MIFNIEFDERAVKEWQKLDNSIREQFKKKLKKLQKNPYIEPARLHGDLACFLRNFSF
Download Length: 174 bp
>T6023 NC_014228:c1844996-1844823 [Xenorhabdus nematophila ATCC 19061]
ATGATATTTAATATCGAGTTTGACGAACGCGCGGTAAAAGAATGGCAAAAGCTGGATAACAGCATTCGTGAACAGTTTAA
AAAGAAGCTGAAAAAGTTACAGAAAAACCCTTATATCGAGCCTGCCAGACTACATGGCGATTTAGCGTGTTTTCTGAGGA
ATTTTTCTTTTTAA
ATGATATTTAATATCGAGTTTGACGAACGCGCGGTAAAAGAATGGCAAAAGCTGGATAACAGCATTCGTGAACAGTTTAA
AAAGAAGCTGAAAAAGTTACAGAAAAACCCTTATATCGAGCCTGCCAGACTACATGGCGATTTAGCGTGTTTTCTGAGGA
ATTTTTCTTTTTAA
Antitoxin
Download Length: 85 a.a. Molecular weight: 9831.21 Da Isoelectric Point: 4.7025
>AT6023 WP_010847387.1 NC_014228:c1845240-1844986 [Xenorhabdus nematophila ATCC 19061]
MSTIQIRVDEELKKSAYQAFDKLNLSPSDAMRLFLRYVAEHEKLPFTEVSVMVAEHDEDEDILDVVRDRLKNPAKRIRVN
LDDI
MSTIQIRVDEELKKSAYQAFDKLNLSPSDAMRLFLRYVAEHEKLPFTEVSVMVAEHDEDEDILDVVRDRLKNPAKRIRVN
LDDI
Download Length: 255 bp
>AT6023 NC_014228:c1845240-1844986 [Xenorhabdus nematophila ATCC 19061]
ATGAGCACCATCCAAATAAGAGTAGATGAAGAGCTTAAGAAAAGTGCATATCAGGCATTTGATAAGCTGAATCTGTCCCC
TTCTGATGCAATGCGACTCTTCCTGCGTTATGTGGCAGAACATGAAAAGCTACCATTTACTGAGGTATCGGTGATGGTTG
CAGAGCACGATGAAGATGAAGATATTCTGGATGTAGTACGCGATCGCCTAAAGAATCCTGCCAAACGAATCCGGGTAAAT
CTCGATGATATTTAA
ATGAGCACCATCCAAATAAGAGTAGATGAAGAGCTTAAGAAAAGTGCATATCAGGCATTTGATAAGCTGAATCTGTCCCC
TTCTGATGCAATGCGACTCTTCCTGCGTTATGTGGCAGAACATGAAAAGCTACCATTTACTGAGGTATCGGTGATGGTTG
CAGAGCACGATGAAGATGAAGATATTCTGGATGTAGTACGCGATCGCCTAAAGAATCCTGCCAAACGAATCCGGGTAAAT
CTCGATGATATTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10166 | Xenorhabdus nematophila ATCC 19061 |
54.348 |
80.702 |
0.439 |
| T1041 | Escherichia coli str. K-12 substr. MG1655 |
47.059 |
89.474 |
0.421 |
| T6132 | Escherichia coli |
45.098 |
89.474 |
0.404 |
| T6131 | Escherichia coli |
45.098 |
89.474 |
0.404 |
| T175 | Vibrio cholerae O1 biovar El Tor str. N16961 |
43.137 |
89.474 |
0.386 |
| T6130 | Erwinia pyrifoliae Ep1/96 |
45.652 |
80.702 |
0.368 |
| T173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
43.478 |
80.702 |
0.351 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1041 | Escherichia coli str. K-12 substr. MG1655 |
41.667 |
100 |
0.443 |
Multiple sequence alignment
References
(1) Lalit Kumar Gautam et al. (2017) Functional annotation of a novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach. Journal of Molecular Modeling 23(6):189. [PubMed:28508139]