57718

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 38817..39352 Replicon plasmid unnamed1
Accession NZ_CP013024
Organism Escherichia coli strain 2009C-3133

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag MJ49_RS00220 Protein ID WP_000222775.1
Coordinates 39065..39352 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag MJ49_RS00215 Protein ID WP_001139207.1
Coordinates 38817..39068 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ49_RS00190 33959..34579 - 621 WP_000176497.1 DUF1449 family protein -
MJ49_RS00195 34846..35511 - 666 WP_000154143.1 hypothetical protein -
MJ49_RS00200 35652..36293 - 642 WP_000335846.1 transcription termination factor NusG -
MJ49_RS00205 36996..37868 + 873 WP_000729440.1 incFII family plasmid replication initiator RepA -
MJ49_RS00215 38817..39068 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
MJ49_RS00220 39065..39352 + 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ49_RS32660 39362..39907 + 546 WP_000926984.1 hypothetical protein -
MJ49_RS00235 40559..41161 - 603 WP_000517694.1 hypothetical protein -
MJ49_RS00240 41515..41754 + 240 Protein_46 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / tet(A) - 1..120611 120611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T57718 WP_000222775.1 NZ_CP013024:39065-39352 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T57718 NZ_CP013024:39065-39352 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT57718 WP_001139207.1 NZ_CP013024:38817-39068 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT57718 NZ_CP013024:38817-39068 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References