99741

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 78700..79235 Replicon plasmid unnamed1
Accession NZ_CP027600
Organism Escherichia coli strain 97-3250

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag C7A06_RS32995 Protein ID WP_000222775.1
Coordinates 78948..79235 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag C7A06_RS32990 Protein ID WP_001139207.1
Coordinates 78700..78951 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A06_RS32940 73842..74462 - 621 WP_000176497.1 DUF1449 family protein -
C7A06_RS32950 74729..75394 - 666 WP_000154143.1 hypothetical protein -
C7A06_RS32955 75535..76176 - 642 WP_000335846.1 transcription termination factor NusG -
C7A06_RS32970 76879..77751 + 873 WP_000729440.1 incFII family plasmid replication initiator RepA -
C7A06_RS34620 78446..78613 + 168 WP_000841010.1 hypothetical protein -
C7A06_RS32990 78700..78951 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
C7A06_RS32995 78948..79235 + 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C7A06_RS34625 79245..79790 + 546 WP_000926984.1 hypothetical protein -
C7A06_RS33020 80442..81044 - 603 WP_000517694.1 hypothetical protein -
C7A06_RS33030 81398..81637 + 240 Protein_84 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaTEM-1B / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) - 1..120604 120604


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T99741 WP_000222775.1 NZ_CP027600:78948-79235 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T99741 NZ_CP027600:78948-79235 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT99741 WP_001139207.1 NZ_CP027600:78700-78951 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT99741 NZ_CP027600:78700-78951 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References