31223

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 38815..39332 Replicon plasmid pMRY16-002_3
Accession NZ_AP017613
Organism Escherichia coli strain 20Ec-P-124

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag CPZ02_RS27380 Protein ID WP_096488308.1
Coordinates 39063..39332 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag CPZ02_RS27375 Protein ID WP_001139207.1
Coordinates 38815..39066 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ02_RS27330 33957..34577 - 621 WP_000176497.1 DUF1449 family protein -
CPZ02_RS27340 34844..35509 - 666 WP_000154143.1 hypothetical protein -
CPZ02_RS27345 35650..36291 - 642 WP_000335846.1 transcription termination factor NusG -
CPZ02_RS27355 36994..37866 + 873 WP_000729440.1 incFII family plasmid replication initiator RepA -
CPZ02_RS27375 38815..39066 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
CPZ02_RS27380 39063..39332 + 270 WP_096488308.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ02_RS27385 39361..40058 + 698 Protein_44 IS1 family transposase -
CPZ02_RS27390 40227..41042 + 816 WP_000018329.1 aminoglycoside O-phosphotransferase APH(3')-Ia -
CPZ02_RS27395 41232..41936 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
CPZ02_RS27400 42001..43251 - 1251 Protein_47 transposase -
CPZ02_RS27410 43326..43994 - 669 WP_001300294.1 EAL domain-containing protein -
CPZ02_RS27415 44030..44266 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid aph(3')-Ia / tet(A) - 1..108957 108957


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10180.12 Da        Isoelectric Point: 10.4752

>T31223 WP_096488308.1 NZ_AP017613:39063-39332 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RGDAANLLI

Download         Length: 270 bp

>T31223 NZ_AP017613:39063-39332 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGGTGATGCTGCCAACTTACTGATTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT31223 WP_001139207.1 NZ_AP017613:38815-39066 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT31223 NZ_AP017613:38815-39066 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References