Detailed information of TA system TA006258

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 1687366..1687999
• Replicon: chromosome
• Accession: NC_011593
• Organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EYF0
• Locus tag: BLON_RS07965
• Protein ID: WP_012577854.1
• Coordinates: 1687366..1687734 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7GS37
• Locus tag: BLON_RS07970
• Protein ID: WP_012577855.1
• Coordinates: 1687715..1687999 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLON_RS07920 (Blon_1529)	1682996..1683253	+	258	WP_012577847.1	hypothetical protein	-
BLON_RS07925 (Blon_1530)	1683250..1683741	+	492	WP_012577848.1	hypothetical protein	-
BLON_RS07930 (Blon_1531)	1683887..1684291	-	405	WP_014484963.1	tyrosine-type recombinase/integrase	-
BLON_RS07935 (Blon_1532)	1684288..1684548	-	261	WP_012577849.1	hypothetical protein	-
BLON_RS07940 (Blon_1533)	1684703..1685047	-	345	WP_012577850.1	hypothetical protein	-
BLON_RS07945 (Blon_1534)	1685178..1685549	-	372	WP_012577851.1	hypothetical protein	-
BLON_RS07950 (Blon_1535)	1685656..1686411	-	756	WP_012577852.1	hypothetical protein	-
BLON_RS07955	1686408..1686635	-	228	WP_014484964.1	hypothetical protein	-
BLON_RS07960 (Blon_1536)	1686632..1687177	-	546	WP_012577853.1	hypothetical protein	-
BLON_RS07965 (Blon_1537)	1687366..1687734	-	369	WP_012577854.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLON_RS07970 (Blon_1538)	1687715..1687999	-	285	WP_012577855.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLON_RS07975 (Blon_1539)	1688075..1688374	-	300	WP_012577856.1	hypothetical protein	-
BLON_RS07980	1688462..1688644	-	183	WP_014484966.1	hypothetical protein	-
BLON_RS07985 (Blon_1540)	1688644..1688985	-	342	WP_012577857.1	hypothetical protein	-
BLON_RS07990 (Blon_1541)	1688987..1689772	-	786	WP_012577858.1	hypothetical protein	-
BLON_RS07995 (Blon_1542)	1689971..1690312	-	342	WP_012577859.1	hypothetical protein	-
BLON_RS08000 (Blon_1543)	1690426..1690770	-	345	WP_014484967.1	hypothetical protein	-
BLON_RS14340 (Blon_1544)	1690767..1690928	-	162	WP_155245937.1	hypothetical protein	-
BLON_RS08005 (Blon_1545)	1691018..1691632	-	615	WP_012577862.1	lysozyme	-
BLON_RS08010	1691845..1692072	-	228	WP_025221588.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_017219 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM Prophage TA026379 1660718..1723279 0 View NZ_LR134354 Bifidobacterium longum subsp. infantis strain NCTC11817 Prophage TA287798 1660722..1723283 0 View NZ_LR655210 Bifidobacterium longum subsp. infantis isolate B.longum_ssp_infantis_6 Prophage TA288654 1365971..1428532 0 View NZ_LR698953 Bifidobacterium longum isolate MGYG-HGUT-01292 Prophage TA288686 1660928..1723489 0	inside	Prophage	-	-	1660928..1723489	62561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (16-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-53)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13866.75 Da
• Isoelectric Point: 4.5746

>T6258 WP_012577854.1 NC_011593:c1687734-1687366 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MTSTPSEPRLYDVWLMWVEFPDHPGIGKPRPVVITEVDGDLVSGIVAKITGNTDWDEAGDVPLLDWKAEGLLKPSLVRCS
QRFYFNRSELLQWFGRLSLRDAEHVNDGLEATLDIPPYRRSV

Nucleotide

• Length: 369 bp

>T6258 NC_011593:c1687734-1687366 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGACCAGTACGCCAAGTGAACCGCGCCTGTATGACGTGTGGCTGATGTGGGTCGAGTTTCCCGACCATCCCGGTATCGG
CAAGCCGCGTCCCGTGGTAATCACCGAGGTTGACGGTGATCTGGTGTCGGGTATCGTGGCGAAGATAACCGGCAACACTG
ATTGGGATGAGGCCGGCGACGTGCCGCTGCTCGACTGGAAGGCCGAGGGGCTGTTGAAGCCGTCACTCGTGCGCTGTTCG
CAACGCTTCTACTTCAACAGGAGCGAACTGCTGCAATGGTTCGGACGACTCTCGTTGAGGGACGCGGAGCATGTTAACGA
CGGATTGGAAGCCACGTTGGACATTCCACCATACAGGCGGAGCGTATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10472.80 Da
• Isoelectric Point: 5.0057

>AT6258 WP_012577855.1 NC_011593:c1687999-1687715 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MGKLVANIDDDVKARAAALYDSMGMSLSTAVNMFLRQSLVDNGLPFKPTRHTPDGYPVPPVHNAYMFERSEKGHVILPAD
WNDSEDAVYDQYAK

Nucleotide

• Length: 285 bp

>AT6258 NC_011593:c1687999-1687715 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGGGTAAGCTGGTCGCCAATATCGATGATGATGTCAAGGCGCGTGCCGCCGCGCTCTACGATTCCATGGGCATGAGCCT
GAGCACCGCAGTCAACATGTTTTTACGCCAGTCTCTGGTGGACAACGGGTTGCCGTTCAAGCCGACGCGGCACACGCCGG
ACGGCTATCCGGTGCCGCCTGTTCACAATGCCTACATGTTCGAGCGTTCGGAGAAGGGCCATGTGATACTGCCCGCCGAT
TGGAATGATTCGGAGGATGCTGTCTATGACCAGTACGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6259	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	44.118	86.441	0.381

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6259	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	33.696	97.872	0.33

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: W6EYF0

Antitoxin

• AlphaFold DB: B7GS37

References

(1) Olga Averina et al. (2015) Functional analysis of the type II toxin-antitoxin systems of the MazEF and RelBE families in Bifidobacterium longum subsp. infantis ATCC 15697. Anaerobe 35(Pt B):59-67. [PubMed:26210151]


 experimental literature