Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 1831993..1832548 | Replicon | chromosome |
| Accession | NC_011593 | ||
| Organism | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | B7GSS5 |
| Locus tag | BLON_RS08710 | Protein ID | WP_003833460.1 |
| Coordinates | 1832225..1832548 (+) | Length | 108 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | S2ZDC4 |
| Locus tag | BLON_RS08705 | Protein ID | WP_003829112.1 |
| Coordinates | 1831993..1832238 (+) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BLON_RS08685 (Blon_1674) | 1827820..1828482 | + | 663 | WP_012577980.1 | TetR/AcrR family transcriptional regulator | - |
| BLON_RS08690 (Blon_1675) | 1828639..1829910 | + | 1272 | WP_012577981.1 | MFS transporter | - |
| BLON_RS08695 | 1830094..1830298 | - | 205 | Protein_1712 | XRE family transcriptional regulator | - |
| BLON_RS08700 (Blon_1677) | 1830563..1831819 | - | 1257 | WP_003829116.1 | HipA domain-containing protein | - |
| BLON_RS08705 (Blon_1678) | 1831993..1832238 | + | 246 | WP_003829112.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| BLON_RS08710 (Blon_1679) | 1832225..1832548 | + | 324 | WP_003833460.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| BLON_RS13745 (Blon_1680) | 1832765..1833379 | - | 615 | WP_012577983.1 | cupin domain-containing protein | - |
| BLON_RS08720 (Blon_1681) | 1833473..1834342 | - | 870 | WP_003829104.1 | class I SAM-dependent methyltransferase | - |
| BLON_RS08725 (Blon_1682) | 1834580..1834945 | + | 366 | WP_012577984.1 | YccF domain-containing protein | - |
| BLON_RS08730 (Blon_1683) | 1835475..1836740 | + | 1266 | WP_012577985.1 | Fic family protein | - |
| BLON_RS08735 (Blon_1684) | 1837210..1837508 | + | 299 | Protein_1720 | IS256 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 108 a.a. Molecular weight: 11829.64 Da Isoelectric Point: 6.2932
>T6260 WP_003833460.1 NC_011593:1832225-1832548 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL
Download Length: 324 bp
>T6260 NC_011593:1832225-1832548 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA
Antitoxin
Download Length: 82 a.a. Molecular weight: 9339.43 Da Isoelectric Point: 4.5881
>AT6260 WP_003829112.1 NC_011593:1831993-1832238 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R
Download Length: 246 bp
>AT6260 NC_011593:1831993-1832238 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10140 | Pseudomonas savastanoi |
33.019 |
99.065 |
0.327 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4R0SL31 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A087B6Q6 |
References
(1) Olga Averina et al. (2015) Functional analysis of the type II toxin-antitoxin systems of the MazEF and RelBE families in Bifidobacterium longum subsp. infantis ATCC 15697. Anaerobe 35(Pt B):59-67. [PubMed:26210151]