Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 11113..11657 | Replicon | plasmid pPsv48B |
| Accession | NC_019266 | ||
| Organism | Pseudomonas savastanoi | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | H1ZWZ2 |
| Locus tag | PSA3335_RS00055 | Protein ID | WP_015060675.1 |
| Coordinates | 11113..11436 (-) | Length | 108 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | H1ZWZ3 |
| Locus tag | PSA3335_RS00060 | Protein ID | WP_015060676.1 |
| Coordinates | 11433..11657 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSA3335_RS00035 (PSA3335_B0007) | 6466..7623 | + | 1158 | WP_015060674.1 | putative DNA binding domain-containing protein | - |
| PSA3335_RS00040 | 7976..8125 | + | 150 | WP_168550519.1 | hypothetical protein | - |
| PSA3335_RS00275 (PSA3335_B0010) | 9501..10901 | + | 1401 | WP_002556090.1 | protein-tyrosine phosphatase family protein | - |
| PSA3335_RS00055 (PSA3335_B0011) | 11113..11436 | - | 324 | WP_015060675.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| PSA3335_RS00060 (PSA3335_B0012) | 11433..11657 | - | 225 | WP_015060676.1 | antitoxin MazE family protein | Antitoxin |
| PSA3335_RS00065 (PSA3335_B0013) | 12220..12873 | + | 654 | WP_015060677.1 | division plane positioning ATPase MipZ | - |
| PSA3335_RS00070 (PSA3335_B0014) | 12922..13233 | + | 312 | WP_168550520.1 | stability/partitioning determinant | - |
| PSA3335_RS00075 (PSA3335_B0016) | 13552..13938 | - | 387 | WP_244312839.1 | hypothetical protein | - |
| PSA3335_RS00080 (PSA3335_B0017) | 14176..14610 | - | 435 | WP_244312841.1 | DUF6392 family protein | - |
| PSA3335_RS00085 (PSA3335_B0018) | 14734..15006 | - | 273 | WP_244312843.1 | helix-turn-helix transcriptional regulator | - |
| PSA3335_RS00090 (PSA3335_B0019) | 15348..15875 | + | 528 | WP_015060683.1 | transcription termination/antitermination NusG family protein | - |
| PSA3335_RS00095 (PSA3335_B0020) | 15889..16155 | + | 267 | WP_228409371.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..45220 | 45220 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 108 a.a. Molecular weight: 11532.58 Da Isoelectric Point: 8.0546
>T10140 WP_015060675.1 NC_019266:c11436-11113 [Pseudomonas savastanoi pv. savastanoi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVPTAENGLQKPSQVMLDKTMTLKREKI
GPTFGHIGVDSMVEVERCLAVFLGIAN
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVPTAENGLQKPSQVMLDKTMTLKREKI
GPTFGHIGVDSMVEVERCLAVFLGIAN
Download Length: 324 bp
>T10140 NC_019266:c11436-11113 [Pseudomonas savastanoi pv. savastanoi]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAAATA
GGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGCCAA
TTGA
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAAATA
GGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGCCAA
TTGA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8515.62 Da Isoelectric Point: 5.5294
>AT10140 WP_015060676.1 NC_019266:c11657-11433 [Pseudomonas savastanoi pv. savastanoi]
MPTSHVNDRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP
MPTSHVNDRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP
Download Length: 225 bp
>AT10140 NC_019266:c11657-11433 [Pseudomonas savastanoi pv. savastanoi]
ATGCCTACATCCCATGTAAATGACCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA
ATGCCTACATCCCATGTAAATGACCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6260 | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 |
33.019 |
99.065 |
0.327 |
| T10042 | Candidatus Desulforudis audaxviator MP104C |
30 |
100 |
0.308 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | H1ZWZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A330JWK7 |
References
(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866]