Detailed information of TA system TA020368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4016365..4017164
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: ECs_4008
• Protein ID: NP_312035.1
• Coordinates: 4016700..4017164 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: ECs_4007
• Protein ID: NP_312034.1
• Coordinates: 4016365..4016700 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_4004	4012150..4012920	-	771	NP_312031.1	alpha-dehydro-beta-deoxy-D-glucarate aldolase	-
ECs_4005	4012936..4014270	-	1335	NP_312032.1	(D)-galactarate transporter	-
ECs_4006	4014645..4016216	+	1572	NP_312033.1	D-galactarate dehydrogenase	-
ECs_4007	4016365..4016700	+	336	NP_312034.1	antitoxin of the SohA(PrlF)-YhaV toxin-antitoxin system	Antitoxin
ECs_4008	4016700..4017164	+	465	NP_312035.1	toxin of the SohB(PrlF)-YhaV toxin-antitoxin system	Toxin
ECs_4009	4017219..4018028	-	810	NP_312036.1	transcriptional repressor of the aga regulon	-
ECs_4010	4018277..4019557	+	1281	NP_312037.1	tagatose-1,6-bisphosphate aldolase	-
ECs_4011	4019580..4020053	+	474	NP_312038.2	N-acetylgalactosamine-specific enzyme IIB component of PTS	-
ECs_4012	4020064..4020843	+	780	NP_312039.1	N-acetylgalactosamine-specific enzyme IIC component of PTS	-
ECs_4013	4020833..4021711	+	879	NP_312040.1	N-acetylgalactosamine-specific PTS system enzyme IID component	-
ECs_4014	4021729..4022163	+	435	NP_312041.1	N-acetyl-galactosamine/galactosamine PTS system enzyme IIA component	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_013941 Escherichia coli O55:H7 str. CB9615 Genomic island TA024622 3897238..3906678 0.002 View NC_017656 Escherichia coli O55:H7 str. RM12579 Genomic island TA027588 3805721..3815161 0.002 View NC_017906 Escherichia coli Xuzhou21 Genomic island TA027790 3934143..3944296 0 View NZ_AP026090 Escherichia coli strain 26_141088 Genomic island TA041974 3938921..3948474 0.002 View NZ_AP026106 Escherichia coli strain CEC01311 Genomic island TA042220 3873499..3882939 0.002 View NZ_AP026110 Escherichia coli strain CEC08123 Genomic island TA042297 3917926..3927966 0 View NZ_AP026116 Escherichia coli strain CEC13002 Genomic island TA042404 3921972..3932012 0 View NZ_CP012802 Escherichia coli O157:H7 strain WS4202 Genomic island TA057234 3879973..3889413 0.002 View NZ_CP018241 Escherichia coli strain 319 Genomic island TA069080 3974881..3984921 0 View NZ_CP018243 Escherichia coli strain 350 Genomic island TA069123 3947741..3957181 0.002 View NZ_CP018247 Escherichia coli strain 7784 Genomic island TA069194 3907400..3917440 0 View NZ_CP021726 Escherichia coli strain Combat11I9 Genomic island TA077170 3288684..3298519 0.009 View NZ_CP021840 Escherichia coli strain EC974 Genomic island TA077591 786195..795916 0.01 View NZ_CP028693 Escherichia coli strain 108 Genomic island TA102813 823768..835048 0.003 View NZ_CP030767 Escherichia coli strain 2017C-4109 Genomic island TA107876 3585812..3594691 0.003 View NZ_CP034797 Escherichia coli strain 08-3918 Genomic island TA116658 2246419..2256459 0 View NZ_CP035366 Escherichia coli O157:H7 strain C1-057 Genomic island TA117641 3984735..3994776 0 View NZ_CP038374 Escherichia coli O157:H7 strain F3113 Genomic island TA122139 4744654..4754807 0 View NZ_CP038376 Escherichia coli O157:H7 strain F1273 Genomic island TA122180 4811528..4820968 0.002 View NZ_CP038428 Escherichia coli O157:H7 strain 611 Genomic island TA122773 3721023..3731176 0 View NZ_CP038494 Escherichia coli O157:H7 strain TT12B Genomic island TA122945 4624384..4633824 0.002 View NZ_CP040307 Escherichia coli strain F1 E4 Genomic island TA125341 4783986..4794026 0 View NZ_CP040309 Escherichia coli strain 21B8 Genomic island TA125384 4689601..4699641 0 View NZ_CP040311 Escherichia coli strain F3398 Genomic island TA125426 4670957..4680997 0 View NZ_CP040313 Escherichia coli strain M7638 Genomic island TA125467 4685973..4696013 0 View NZ_CP040314 Escherichia coli strain MA11 Genomic island TA125508 4748836..4758876 0 View NZ_CP040316 Escherichia coli strain CV261 Genomic island TA125550 4729143..4739183 0 View NZ_CP045863 Escherichia coli O157:H7 strain ATCC 43890 Genomic island TA142884 3669111..3679264 0 View NZ_CP062160 Escherichia coli strain 20R2R Genomic island TA173458 5056164..5066779 0.002 View NZ_CP062752 Escherichia coli O157:H7 strain Z910 Genomic island TA176168 829081..838634 0.002 View NZ_CP062774 Escherichia coli O157:H7 strain Z563 Genomic island TA176455 828481..838634 0 View NZ_CP065607 Escherichia coli strain FDAARGOS_946 Genomic island TA182959 1579458..1588899 0.002 View NZ_CP082835 Escherichia coli strain 1613 Genomic island TA215387 4031782..4041222 0.002 View NZ_CP103864 Escherichia coli O157:H7 strain RV06 Genomic island TA256849 1319312..1329465 0 View NZ_CP104639 Escherichia coli strain PNUSAE014970 Genomic island TA258713 1338373..1348526 0	inside	Genomic island	-	-	4007011..4017164	10153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T20368 NP_312035.1 NC_002695:4016700-4017164 [Escherichia coli O157:H7 str. Sakai]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T20368 NC_002695:4016700-4017164 [Escherichia coli O157:H7 str. Sakai]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT20368 NP_312034.1 NC_002695:4016365-4016700 [Escherichia coli O157:H7 str. Sakai]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT20368 NC_002695:4016365-4016700 [Escherichia coli O157:H7 str. Sakai]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA