Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 837835..838634 Replicon chromosome
Accession NZ_CP062774
Organism Escherichia coli O157:H7 strain Z563

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ILQ31_RS04080 Protein ID WP_000347273.1
Coordinates 838170..838634 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag ILQ31_RS04075 Protein ID WP_001302819.1
Coordinates 837835..838170 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ31_RS04060 833620..834390 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
ILQ31_RS04065 834406..835740 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ILQ31_RS04070 836115..837686 + 1572 WP_001273776.1 galactarate dehydratase -
ILQ31_RS04075 837835..838170 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ILQ31_RS04080 838170..838634 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ILQ31_RS04085 838689..839498 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ILQ31_RS04090 839747..841027 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
ILQ31_RS04095 841050..841523 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
ILQ31_RS04100 841534..842313 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
ILQ31_RS04105 842303..843181 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
ILQ31_RS04110 843199..843633 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 828481..838634 10153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T176455 WP_000347273.1 NZ_CP062774:838170-838634 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T176455 NZ_CP082821:c5724027-5723617 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGTCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGTCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT176455 WP_001302819.1 NZ_CP062774:837835-838170 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT176455 NZ_CP082821:c5724257-5724027 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGTGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References