Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3678465..3679264 Replicon chromosome
Accession NZ_CP045863
Organism Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GIX96_RS18445 Protein ID WP_000347273.1
Coordinates 3678800..3679264 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag GIX96_RS18440 Protein ID WP_001302819.1
Coordinates 3678465..3678800 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIX96_RS18425 3674250..3675020 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GIX96_RS18430 3675036..3676370 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GIX96_RS18435 3676745..3678316 + 1572 WP_001273776.1 galactarate dehydratase -
GIX96_RS18440 3678465..3678800 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GIX96_RS18445 3678800..3679264 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GIX96_RS18450 3679319..3680128 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GIX96_RS18455 3680377..3681657 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
GIX96_RS18460 3681680..3682153 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
GIX96_RS18465 3682164..3682943 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GIX96_RS18470 3682933..3683811 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GIX96_RS18475 3683829..3684263 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3669111..3679264 10153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T142884 WP_000347273.1 NZ_CP045863:3678800-3679264 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T142884 NZ_CP060893:c44452-44072 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT142884 WP_001302819.1 NZ_CP045863:3678465-3678800 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT142884 NZ_CP060893:c44673-44452 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTACTGAAGTGCTCAACAATGTTGAAGCTGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References