Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3297720..3298519 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag CDC27_RS16790 Protein ID WP_000347273.1
Coordinates 3298055..3298519 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CDC27_RS16785 Protein ID WP_001307405.1
Coordinates 3297720..3298055 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS16770 3293504..3294274 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
CDC27_RS16775 3294290..3295624 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
CDC27_RS16780 3295999..3297570 + 1572 WP_072685993.1 galactarate dehydratase -
CDC27_RS16785 3297720..3298055 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CDC27_RS16790 3298055..3298519 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CDC27_RS16795 3298574..3299383 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
CDC27_RS16805 3299632..3300912 + 1281 WP_033814994.1 tagatose-bisphosphate aldolase subunit KbaZ -
CDC27_RS16810 3300935..3301408 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
CDC27_RS16815 3301419..3302198 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CDC27_RS16820 3302188..3303066 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CDC27_RS16825 3303084..3303518 + 435 WP_033814993.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3288684..3298519 9835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T77170 WP_000347273.1 NZ_CP021726:3298055-3298519 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T77170 NZ_CP021726:3298055-3298519 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT77170 WP_001307405.1 NZ_CP021726:3297720-3298055 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT77170 NZ_CP021726:3297720-3298055 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References