Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5065980..5066779 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IEM32_RS25005 Protein ID WP_000347273.1
Coordinates 5066315..5066779 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag IEM32_RS25000 Protein ID WP_001302819.1
Coordinates 5065980..5066315 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS24985 5061765..5062535 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IEM32_RS24990 5062551..5063885 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IEM32_RS24995 5064260..5065831 + 1572 WP_001273776.1 galactarate dehydratase -
IEM32_RS25000 5065980..5066315 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IEM32_RS25005 5066315..5066779 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IEM32_RS25010 5066834..5067643 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IEM32_RS25015 5067892..5069172 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
IEM32_RS25020 5069195..5069668 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
IEM32_RS25025 5069679..5070458 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IEM32_RS25030 5070448..5071326 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IEM32_RS25035 5071344..5071778 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 5056164..5066779 10615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T173458 WP_000347273.1 NZ_CP062160:5066315-5066779 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T173458 NZ_CP080222:c2512871-2512605 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT173458 WP_001302819.1 NZ_CP062160:5065980-5066315 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT173458 NZ_CP080222:c2513122-2512871 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References