Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1588100..1588899 Replicon chromosome
Accession NZ_CP065607
Organism Escherichia coli strain FDAARGOS_946

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag I6H03_RS08235 Protein ID WP_000347273.1
Coordinates 1588435..1588899 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag I6H03_RS08230 Protein ID WP_001302819.1
Coordinates 1588100..1588435 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H03_RS08215 1583885..1584655 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6H03_RS08220 1584671..1586005 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
I6H03_RS08225 1586380..1587951 + 1572 WP_001273776.1 galactarate dehydratase -
I6H03_RS08230 1588100..1588435 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6H03_RS08235 1588435..1588899 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6H03_RS08240 1588954..1589763 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6H03_RS08245 1590012..1591292 + 1281 WP_000681946.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6H03_RS08250 1591315..1591788 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6H03_RS08255 1591799..1592578 + 780 WP_000406210.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
I6H03_RS08260 1592568..1593446 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
I6H03_RS08265 1593464..1593898 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1579458..1588899 9441


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T182959 WP_000347273.1 NZ_CP065607:1588435-1588899 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T182959 NZ_CP086623:3057034-3057141 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT182959 WP_001302819.1 NZ_CP065607:1588100-1588435 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT182959 NZ_CP086623:c3056984-3056921 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References