Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 795117..795916 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name yhaV Uniprot ID A0A246NUP1
Locus tag CDH88_RS04045 Protein ID WP_021577991.1
Coordinates 795452..795916 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CDH88_RS04040 Protein ID WP_001307405.1
Coordinates 795117..795452 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS04025 790902..791672 - 771 WP_021577990.1 2-dehydro-3-deoxyglucarate aldolase -
CDH88_RS04030 791688..793022 - 1335 WP_000599633.1 galactarate/glucarate/glycerate transporter GarP -
CDH88_RS04035 793397..794968 + 1572 WP_001273763.1 galactarate dehydratase -
CDH88_RS04040 795117..795452 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CDH88_RS04045 795452..795916 + 465 WP_021577991.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CDH88_RS04050 795971..796780 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CDH88_RS04060 797029..798309 + 1281 WP_021577992.1 tagatose-bisphosphate aldolase subunit KbaZ -
CDH88_RS04065 798332..798805 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
CDH88_RS04070 798816..799595 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CDH88_RS04075 799585..800463 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CDH88_RS04080 800481..800915 + 435 WP_000948826.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 786195..795916 9721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T77591 WP_021577991.1 NZ_CP021840:795452-795916 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKILAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T77591 NZ_CP021840:795452-795916 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGATATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT77591 WP_001307405.1 NZ_CP021840:795117-795452 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT77591 NZ_CP021840:795117-795452 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCTTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NUP1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References