Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1328666..1329465 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NUE47_RS06555 Protein ID WP_000347273.1
Coordinates 1329001..1329465 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag NUE47_RS06550 Protein ID WP_001302819.1
Coordinates 1328666..1329001 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS06535 (1324451) 1324451..1325221 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NUE47_RS06540 (1325237) 1325237..1326571 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NUE47_RS06545 (1326946) 1326946..1328517 + 1572 WP_001273776.1 galactarate dehydratase -
NUE47_RS06550 (1328666) 1328666..1329001 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NUE47_RS06555 (1329001) 1329001..1329465 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NUE47_RS06560 (1329520) 1329520..1330329 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NUE47_RS06565 (1330578) 1330578..1331858 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
NUE47_RS06570 (1331881) 1331881..1332354 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
NUE47_RS06575 (1332365) 1332365..1333144 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NUE47_RS06580 (1333134) 1333134..1334012 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NUE47_RS06585 (1334030) 1334030..1334464 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1319312..1329465 10153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T256849 WP_000347273.1 NZ_CP103864:1329001-1329465 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT256849 WP_001302819.1 NZ_CP103864:1328666-1329001 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References