Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4040423..4041222 Replicon chromosome
Accession NZ_CP082835
Organism Escherichia coli strain 1613

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K8O78_RS20375 Protein ID WP_000347273.1
Coordinates 4040758..4041222 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag K8O78_RS20370 Protein ID WP_001302819.1
Coordinates 4040423..4040758 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8O78_RS20355 (4036208) 4036208..4036978 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
K8O78_RS20360 (4036994) 4036994..4038328 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K8O78_RS20365 (4038703) 4038703..4040274 + 1572 WP_001273776.1 galactarate dehydratase -
K8O78_RS20370 (4040423) 4040423..4040758 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8O78_RS20375 (4040758) 4040758..4041222 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8O78_RS20380 (4041277) 4041277..4042086 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8O78_RS20385 (4042335) 4042335..4043615 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8O78_RS20390 (4043638) 4043638..4044111 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8O78_RS20395 (4044122) 4044122..4044901 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8O78_RS20400 (4044891) 4044891..4045769 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K8O78_RS20405 (4045787) 4045787..4046221 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4031782..4041222 9440


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T215387 WP_000347273.1 NZ_CP082835:4040758-4041222 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T215387 NZ_CP110674:361812-362114 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT215387 WP_001302819.1 NZ_CP082835:4040423-4040758 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT215387 NZ_CP110674:361588-361815 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References