Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00127  |
| T6SS type | Type i1 |
| Strain | Myxococcus xanthus DK 1622 |
| Replicon (RefSeq) | chromosome (NC_008095) |
| Location | 5996770..6055445 |
| Function | effector translocation |
| Comment | A deletion covering MXAN_4807-MXAN_4813 shows no growth defects or motility defects; however, the mutant is unable to form mature fruiting bodies (A. Konovalova, unpublished data). |
| References | [1] Chang YW et al (2014) Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 11(7):737-9. [PudMed:24813625] [2] Boyer F et al (2009) Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 0.488888889. [PudMed:19284603] [3] Konovalova A et al (2010) Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 34(2):89-106. [PudMed:19895646] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TagL | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 5 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| MXAN_RS23295 | 5992055..5993263 | + | 1209 | WP_011554780.1 | ceramide glucosyltransferase | ||
| MXAN_RS23300 | 5993383..5993763 | + | 381 | WP_171452214.1 | response regulator | ||
| MXAN_RS23305 | 5993842..5995125 | + | 1284 | WP_011554782.1 | hemolysin family protein | ||
| MXAN_RS23310 | 5995148..5996368 | - | 1221 | WP_020478478.1 | fibronectin type III domain-containing protein | ||
| MXAN_RS23320 | 5996770..6001965 | - | 5196 | WP_011554784.1 | hypothetical protein | ||
| T6CP011373 | TagL | MXAN_RS23325 | 6001993..6003840 | - | 1848 | WP_011554785.1 | OmpA family protein |
| T6CP001230 | TssI | MXAN_RS23330 (tssI) | 6004071..6006335 | + | 2265 | WP_011554786.1 | type VI secretion system tip protein VgrG |
| T6CP001231 | TssJ | MXAN_RS23335 (tssJ) | 6006481..6007098 | + | 618 | WP_011554787.1 | type VI secretion system lipoprotein TssJ |
| T6CP001232 | TssK | MXAN_RS23340 (tssK) | 6007095..6008462 | + | 1368 | WP_011554788.1 | type VI secretion system baseplate subunit TssK |
| T6CP001233 | TssL | MXAN_RS23345 | 6008478..6009194 | + | 717 | WP_011554789.1 | DotU family type IV/VI secretion system protein |
| T6CP001234 | TssM | MXAN_RS23350 (tssM) | 6009191..6012886 | + | 3696 | WP_011554790.1 | type VI secretion system membrane subunit TssM |
| MXAN_RS23355 (tagF) | 6012888..6013820 | + | 933 | WP_011554791.1 | type VI secretion system-associated protein TagF | ||
| T6CP009587 | TssA | MXAN_RS23360 (tssA) | 6013836..6015860 | + | 2025 | WP_011554792.1 | type VI secretion system protein TssA |
| T6CP001235 | TssB | MXAN_RS23365 (tssB) | 6015943..6016437 | + | 495 | WP_011554793.1 | type VI secretion system contractile sheath small subunit |
| T6CP001236 | TssC | MXAN_RS23370 (tssC) | 6016463..6017947 | + | 1485 | WP_011554794.1 | type VI secretion system contractile sheath large subunit |
| T6CP009585 | TssD | MXAN_RS23375 (hcp) | 6018067..6018558 | + | 492 | WP_011554795.1 | type VI secretion system tube protein Hcp |
| T6CP009584 | TssE | MXAN_RS23380 (tssE) | 6018642..6019037 | + | 396 | WP_011554796.1 | type VI secretion system baseplate subunit TssE |
| T6CP001237 | TssF | MXAN_RS23385 (tssF) | 6019075..6020826 | + | 1752 | WP_011554797.1 | type VI secretion system baseplate subunit TssF |
| T6CP001238 | TssG | MXAN_RS23390 (tssG) | 6020790..6021833 | + | 1044 | WP_020478477.1 | type VI secretion system baseplate subunit TssG |
| T6CP001239 | TssH | MXAN_RS23395 (tssH) | 6021917..6024574 | + | 2658 | WP_011554799.1 | type VI secretion system ATPase TssH |
| MXAN_RS23400 | 6024583..6025677 | + | 1095 | WP_011554800.1 | hypothetical protein | ||
| MXAN_RS23405 | 6025827..6026192 | + | 366 | WP_011554801.1 | HPF/RaiA family ribosome-associated protein | ||
| MXAN_RS23410 | 6026252..6026809 | + | 558 | WP_011554802.1 | redoxin domain-containing protein | ||
| MXAN_RS23415 | 6026828..6028240 | - | 1413 | WP_020478476.1 | glycoside hydrolase family 16 protein | ||
| MXAN_RS23420 | 6028288..6029355 | - | 1068 | WP_011554804.1 | sulfate/molybdate ABC transporter ATP-binding protein | ||
| MXAN_RS23425 (cysW) | 6029352..6030200 | - | 849 | WP_020478475.1 | sulfate ABC transporter permease subunit CysW | ||
| MXAN_RS23430 (cysT) | 6030206..6031048 | - | 843 | WP_011554806.1 | sulfate ABC transporter permease subunit CysT | ||
| MXAN_RS23435 | 6031061..6032071 | - | 1011 | WP_011554807.1 | sulfate ABC transporter substrate-binding protein | ||
| T6CP001240 | TssH | MXAN_RS23440 | 6032464..6035913 | + | 3450 | WP_011554809.1 | AAA family ATPase |
| T6CP001241 | TssH | MXAN_RS23445 | 6035918..6039301 | + | 3384 | WP_011554810.1 | ATP-dependent Clp protease ATP-binding subunit |
| T6CP001242 | TssH | MXAN_RS23450 | 6039303..6041597 | + | 2295 | WP_020478473.1 | ATP-dependent Clp protease ATP-binding subunit |
| MXAN_RS23455 | 6041643..6042224 | - | 582 | WP_011554812.1 | superoxide dismutase | ||
| MXAN_RS23460 | 6042250..6042750 | - | 501 | WP_011554813.1 | flavin reductase family protein | ||
| MXAN_RS23465 | 6043111..6045372 | - | 2262 | WP_011554815.1 | xanthine dehydrogenase family protein molybdopterin-binding subunit | ||
| MXAN_RS23470 | 6045383..6045844 | - | 462 | WP_011554816.1 | (2Fe-2S)-binding protein | ||
| MXAN_RS23475 | 6045841..6046494 | - | 654 | WP_026114169.1 | hypothetical protein | ||
| T6CP001243 | TssH | MXAN_RS23480 | 6046572..6048992 | - | 2421 | WP_202945165.1 | ATP-dependent Clp protease ATP-binding subunit |
| MXAN_RS23485 | 6049391..6049825 | + | 435 | WP_011554819.1 | hypothetical protein | ||
| MXAN_RS23490 | 6049900..6050466 | + | 567 | WP_011554821.1 | hypothetical protein | ||
| MXAN_RS23495 | 6050649..6052112 | + | 1464 | WP_011554822.1 | MFS transporter | ||
| MXAN_RS23500 | 6052139..6053617 | - | 1479 | WP_011554823.1 | glycoside hydrolase family 6 protein | ||
| MXAN_RS23505 | 6053772..6055445 | - | 1674 | WP_011554824.1 | amidase | ||
| MXAN_RS23510 | 6055602..6057587 | + | 1986 | WP_011554825.1 | ATP-dependent DNA helicase | ||
| MXAN_RS23515 | 6057594..6058550 | - | 957 | WP_020478471.1 | pirin family protein | ||
| MXAN_RS23520 | 6058815..6060029 | - | 1215 | WP_011554827.1 | protein kinase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01476 |
MXAN_5572 (TsxE) | Tae | IMU00302 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 6920542..6922551 (-) | 108462560 |
| EFF01483 |
MXAN_RS00265 | Tde | IMU00308 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 57099..57971 (-) | 499869465 |
| EFF01808 |
MXAN_RS36995 (PAAR-CTD) | Tde | IMU00377 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 6338694..6339836 (-) | 1174098909 |
| EFF01809 |
MXAN_RS08765 (PAAR-CTD) | Tse | IMU00378 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 2144359..2145612 (-) | 499871184 |
| EFF01313 |
MXAN_4809 (hcp) | - | - | Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 6018067..6018558 (+) | 108762846 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00302 |
MXAN_5571 (TsxI) | EFF01476 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 6919379..6920530 (-) | 108762153 |
| IMU00308 |
MXAN_RS00260 | EFF01483 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 56489..57073 (-) | 499869464 |
| IMU00377 |
MXAN_RS24590 | EFF01808 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 6337957..6338418 (-) | 521967099 |
| IMU00378 |
MXAN_RS08760 | EFF01809 |
Myxococcus xanthus DK 1622 | chromosome (NC_008095) | 2143618..2143965 (-) | 521967337 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|