SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001235
Component protein type	TssB
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (165 a.a.); Nucleotide sequence (495 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23365
Coordinate (Strand)	6015943..6016437 (+)
NCBI ID	WP_011554793.1
RefSeq	NC_008095
Uniprot ID	Q1D305_MYXXD, A0A4Y6B727_MYXXA, A0A511HEX7_9DELT
KEGG ID	mxa:MXAN_4807
PDB ID	5URX, 5URW

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	2.6e-54	6..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-164	MSKESSVAPTERVNIVYKPATGNAQEQVELPLKVLMLGDFTGQEDARPLEQRAPINVDKANFNEVMAQQNLKVTLTAADKLSADPNATMNVSLQFKNLNDFSPESVVNQVPELKKLLELRSALNALKGPLGNLPAFRKKLQALLADEDGRKALIKELGLTEETK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9901	-	-

Protein sequence: 165 a.a.

						>T6CP001235 NC_008095:6015943-6016437 [Myxococcus xanthus DK 1622] [TssB]
MSKESSVAPTERVNIVYKPATGNAQEQVELPLKVLMLGDFTGQEDARPLEQRAPINVDKANFNEVMAQQNLKVTLTAADK
LSADPNATMNVSLQFKNLNDFSPESVVNQVPELKKLLELRSALNALKGPLGNLPAFRKKLQALLADEDGRKALIKELGLT
EETK*					

Nucleotide sequence: 495 bp

						>T6CP001235 NC_008095:6015943-6016437 [Myxococcus xanthus DK 1622] [TssB]
ATGAGCAAAGAGAGTTCCGTCGCCCCTACCGAGCGCGTCAACATCGTCTACAAGCCCGCCACCGGCAACGCGCAGGAGCA
GGTGGAGCTGCCGCTGAAGGTGCTGATGCTGGGGGACTTCACGGGTCAGGAAGACGCCCGTCCGCTGGAGCAGCGCGCGC
CCATCAACGTGGACAAGGCCAACTTCAACGAAGTCATGGCCCAGCAGAACCTCAAGGTCACCCTGACCGCCGCCGACAAG
CTTTCGGCGGACCCGAACGCCACGATGAACGTGTCGCTCCAGTTCAAGAACCTGAATGACTTTTCGCCCGAGAGCGTCGT
CAATCAGGTGCCGGAGCTGAAGAAGCTGCTCGAGCTGCGCAGCGCGCTCAACGCCCTCAAGGGCCCCCTGGGCAACCTGC
CGGCGTTCCGCAAGAAGCTCCAGGCTCTGCTCGCCGACGAGGACGGACGCAAGGCGCTCATCAAGGAACTGGGTCTGACC
GAAGAGACCAAGTAG					