SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001231
Component protein type	TssJ
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (206 a.a.); Nucleotide sequence (618 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23335
Coordinate (Strand)	6006481..6007098 (+)
NCBI ID	WP_011554787.1
RefSeq	NC_008095
Uniprot ID	Q1D311_MYXXD, A0A7Y4HFW5_MYXXA
KEGG ID	mxa:MXAN_4801
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.1e-22	41..144

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-205	VWSAGRNLRWGVVIALAAALSGSAGCVKRVPQACETPPPFQVVVDASEQLNPDARGRSLPTVVQIVQLKDSVRLERAGFKDLWGKPEEFLKEDLLQVAELVIPPGRQVKRWVQRDPKARFVLAMGHFRQPLGYSWRTVAALPVVEEARCVERPAGDQGDPKPGDEVFRYRLQGYQIDLMFRPMTQAPEPRLQPQAGDGVSPRRGV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9770	pos: 25-26. SAG-CV.	VWSAGRNLRWGVVIALAAALSGSAG

Protein sequence: 206 a.a.

						>T6CP001231 NC_008095:6006481-6007098 [Myxococcus xanthus DK 1622] [TssJ]
VWSAGRNLRWGVVIALAAALSGSAGCVKRVPQACETPPPFQVVVDASEQLNPDARGRSLPTVVQIVQLKDSVRLERAGFK
DLWGKPEEFLKEDLLQVAELVIPPGRQVKRWVQRDPKARFVLAMGHFRQPLGYSWRTVAALPVVEEARCVERPAGDQGDP
KPGDEVFRYRLQGYQIDLMFRPMTQAPEPRLQPQAGDGVSPRRGV*					

Nucleotide sequence: 618 bp

						>T6CP001231 NC_008095:6006481-6007098 [Myxococcus xanthus DK 1622] [TssJ]
GTGTGGAGTGCCGGCCGGAACCTCCGGTGGGGTGTGGTCATCGCGCTCGCGGCCGCCTTGAGCGGGAGCGCGGGCTGCGT
GAAGCGTGTGCCCCAGGCGTGTGAGACGCCGCCGCCGTTCCAGGTGGTGGTGGATGCGTCGGAGCAGCTCAATCCCGACG
CGCGCGGGCGCTCGCTGCCCACCGTGGTGCAGATCGTCCAGCTCAAGGACAGCGTCCGGCTGGAGCGCGCGGGCTTCAAG
GACCTGTGGGGCAAGCCGGAGGAGTTCCTCAAGGAGGACCTGCTCCAGGTGGCCGAGCTGGTGATTCCGCCCGGCCGCCA
GGTGAAGCGCTGGGTGCAGCGTGATCCGAAGGCCCGCTTCGTCCTGGCCATGGGGCACTTCCGGCAGCCGCTGGGCTATT
CGTGGCGAACGGTGGCGGCGCTCCCCGTGGTGGAGGAAGCGCGCTGCGTGGAGCGCCCCGCGGGTGACCAGGGCGACCCG
AAGCCGGGGGACGAGGTCTTCCGCTACCGGCTGCAAGGCTATCAGATTGACCTGATGTTCCGGCCCATGACGCAGGCGCC
GGAGCCTCGACTCCAACCCCAAGCAGGCGACGGCGTGTCGCCGCGGAGAGGTGTATGA					