SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001240
Component protein type	TssH
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (1150 a.a.); Nucleotide sequence (3450 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23440
Coordinate (Strand)	6032464..6035913 (+)
NCBI ID	WP_011554809.1
RefSeq	NC_008095
Uniprot ID	Q1D2Y9_MYXXD, A0A7Y4HE70_MYXXA
KEGG ID	mxa:MXAN_4823
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	8.3e-30	560..723
ClpB_D2-small	PF10431.11	4.9e-11	753..800
AAA_16	PF13191.8	2.5e-06	269..365

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1149	MPSRTAPFSPESSPMNFRFQCWLQRHVSGRVTLTPLALRRLAVHADSLEAATEELTLALDDQLSRVHPRRVPEFIAPTGGALETVELPAIPVWGAEENHFAPLTLSAVVAPTLNAFLGLHAPRLNSHHWFQSKTLPAEASELLGDRLEGMSDAERLALRPDGPESLLEIEVRATPVALSELTPRELHRNIRPPPRPPDAPADLDDDTREDGEDEDTEALDLDSWEPRRRTAHRGPGEPPAKPPPTPTLDSVGVAWHRLAQEGQLDAAYEQDALVALLRTRLAAKDAEPVVLVGPSGVGKTSVLHALAQSLRAPTATEAERARPFYFLDGSRLIAGEGFFGDWQQQVLRAFREATEARALLSLGHAVELLDAGKSAHSDQNVAQLLLPLLAARDVAVMAEATEETWAQVEQRNTSFARLFSVVRVAEPTPDALGRIVAKVAEDDALAAAMQVQPDALDEARFLCRRFLPYGAQVGNTVAFVRRLLASCAQASRTSVTRLEATRQFASESGIPEHLLRDDQPLASAHVRDFLASRVMGQDAAVERAASVVSVLKAGMSDVRRPLGVLLFVGPTGVGKTELSKALAELLFGAKERMVRLDMGEYAGPDALLRLMGDGETPGYLTSAVRRQPFCVVLLDEVEKAHPAVHDALLGVLGEGRLTDASGRFTDFRNALIILTSNLGADTLRARVGFDASGGAPDMASLRAHYLSEVQRFFRPEMFNRMDDVVVFSPLSAPLLRRLVVRELEAVCRRPGLSLHDALLEVTPAAQDWLALRGFDARYGARPLKRALERELVVPVADWLAAHPRMGPANLTVDAGTTGLELRAESLGGAAEGGGREAIERILEEAATVRAEVQRWSRCAPMHSLRRVLAVFDKVSRHTAYWEERALAEENSRKSSEARALEQSLRECAQQAEAIEDLLFEAHLSRTVDQVESLSREVSQVKAAFARIRQRIYSSLFPPSHGATLYLVPGRGAWRQARMLAAAYEGWAQKASMEKRRGLLAVPERKSGEKAHRTPPPPQWRWADDETFDKLVVQPVAYAIQVKGGPHALLLAGEHGLHRFIEGSQISMVHAYFEPRPLSLFDLSSREALQKQLPKNEVRQVRLSGSGSGQGTLTDLRTGVQQRFGLEGPDLEPLLEPWMQWRVFNSRDED

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9391	-	-

Protein sequence: 1150 a.a.

						>T6CP001240 NC_008095:6032464-6035913 [Myxococcus xanthus DK 1622] [TssH]
MPSRTAPFSPESSPMNFRFQCWLQRHVSGRVTLTPLALRRLAVHADSLEAATEELTLALDDQLSRVHPRRVPEFIAPTGG
ALETVELPAIPVWGAEENHFAPLTLSAVVAPTLNAFLGLHAPRLNSHHWFQSKTLPAEASELLGDRLEGMSDAERLALRP
DGPESLLEIEVRATPVALSELTPRELHRNIRPPPRPPDAPADLDDDTREDGEDEDTEALDLDSWEPRRRTAHRGPGEPPA
KPPPTPTLDSVGVAWHRLAQEGQLDAAYEQDALVALLRTRLAAKDAEPVVLVGPSGVGKTSVLHALAQSLRAPTATEAER
ARPFYFLDGSRLIAGEGFFGDWQQQVLRAFREATEARALLSLGHAVELLDAGKSAHSDQNVAQLLLPLLAARDVAVMAEA
TEETWAQVEQRNTSFARLFSVVRVAEPTPDALGRIVAKVAEDDALAAAMQVQPDALDEARFLCRRFLPYGAQVGNTVAFV
RRLLASCAQASRTSVTRLEATRQFASESGIPEHLLRDDQPLASAHVRDFLASRVMGQDAAVERAASVVSVLKAGMSDVRR
PLGVLLFVGPTGVGKTELSKALAELLFGAKERMVRLDMGEYAGPDALLRLMGDGETPGYLTSAVRRQPFCVVLLDEVEKA
HPAVHDALLGVLGEGRLTDASGRFTDFRNALIILTSNLGADTLRARVGFDASGGAPDMASLRAHYLSEVQRFFRPEMFNR
MDDVVVFSPLSAPLLRRLVVRELEAVCRRPGLSLHDALLEVTPAAQDWLALRGFDARYGARPLKRALERELVVPVADWLA
AHPRMGPANLTVDAGTTGLELRAESLGGAAEGGGREAIERILEEAATVRAEVQRWSRCAPMHSLRRVLAVFDKVSRHTAY
WEERALAEENSRKSSEARALEQSLRECAQQAEAIEDLLFEAHLSRTVDQVESLSREVSQVKAAFARIRQRIYSSLFPPSH
GATLYLVPGRGAWRQARMLAAAYEGWAQKASMEKRRGLLAVPERKSGEKAHRTPPPPQWRWADDETFDKLVVQPVAYAIQ
VKGGPHALLLAGEHGLHRFIEGSQISMVHAYFEPRPLSLFDLSSREALQKQLPKNEVRQVRLSGSGSGQGTLTDLRTGVQ
QRFGLEGPDLEPLLEPWMQWRVFNSRDED*					

Nucleotide sequence: 3450 bp

						>T6CP001240 NC_008095:6032464-6035913 [Myxococcus xanthus DK 1622] [TssH]
ATGCCGTCACGCACGGCACCGTTTTCACCCGAGTCCTCACCCATGAACTTCCGTTTTCAATGCTGGTTGCAGCGGCACGT
CAGCGGCCGGGTGACGCTCACGCCGCTGGCGCTGCGCCGTCTCGCGGTGCACGCGGACTCGCTGGAGGCCGCCACCGAGG
AGCTGACGCTCGCGCTGGACGACCAACTCTCCCGCGTCCACCCGCGGCGCGTGCCGGAGTTCATCGCCCCCACCGGAGGG
GCGCTCGAAACGGTGGAGCTACCCGCGATTCCCGTCTGGGGCGCCGAGGAGAATCACTTCGCGCCGCTGACGTTGTCCGC
CGTGGTGGCCCCCACGCTCAATGCCTTCCTGGGCCTCCACGCGCCACGCCTCAACAGCCATCACTGGTTCCAGAGCAAGA
CACTCCCCGCCGAGGCCTCGGAGCTGCTGGGAGATCGCCTGGAGGGAATGTCCGACGCGGAGCGCCTTGCCCTGCGTCCG
GATGGACCTGAGTCCCTGCTGGAAATCGAAGTGCGGGCCACCCCCGTGGCCCTGTCGGAGCTCACGCCTCGCGAGCTGCA
CCGGAACATTCGTCCACCGCCGCGCCCTCCAGACGCACCCGCCGACCTGGATGACGACACGCGCGAGGACGGCGAGGACG
AAGACACGGAGGCGCTGGACCTGGACAGCTGGGAGCCACGCCGCCGCACCGCGCATCGAGGCCCGGGTGAGCCGCCCGCG
AAGCCGCCGCCTACGCCCACCCTGGACAGCGTCGGCGTGGCCTGGCACCGGCTCGCGCAGGAAGGACAGCTCGACGCGGC
CTACGAGCAGGATGCGCTCGTCGCCCTGCTCCGGACCCGGCTCGCGGCCAAGGACGCCGAACCGGTGGTCCTGGTGGGGC
CTTCGGGCGTGGGCAAGACGTCGGTGCTCCACGCGCTCGCCCAGTCGCTGCGCGCCCCCACCGCCACCGAGGCCGAGCGC
GCCCGCCCGTTCTACTTCCTCGACGGAAGCCGGCTCATCGCGGGCGAGGGCTTCTTCGGAGACTGGCAGCAGCAGGTGCT
GCGCGCCTTCCGTGAGGCCACCGAGGCCCGGGCCCTGCTGTCCCTGGGCCATGCCGTGGAGCTCCTCGACGCGGGCAAGA
GCGCCCACAGTGACCAGAACGTGGCCCAACTGCTGCTCCCGCTCCTCGCGGCGCGTGACGTGGCCGTCATGGCCGAGGCC
ACGGAGGAGACCTGGGCCCAGGTGGAGCAGCGCAACACCAGCTTCGCCCGGCTCTTCTCGGTGGTGCGCGTCGCGGAGCC
CACGCCCGATGCGCTCGGCCGCATCGTCGCCAAGGTGGCGGAGGACGACGCCTTGGCCGCCGCGATGCAGGTCCAGCCCG
ACGCGCTCGACGAGGCGCGCTTCCTGTGCCGCCGCTTCCTGCCCTACGGTGCCCAGGTGGGCAACACCGTGGCCTTCGTG
CGCCGGCTGCTGGCGTCCTGTGCCCAAGCCTCGCGCACCTCCGTCACCCGACTGGAGGCCACCCGCCAATTCGCCTCGGA
GTCCGGCATCCCCGAGCACCTGCTCCGGGACGACCAGCCGCTGGCGTCCGCACACGTGCGCGACTTCCTGGCCTCGCGAG
TGATGGGGCAGGACGCCGCCGTGGAGCGCGCAGCCTCGGTGGTATCCGTCCTCAAGGCCGGCATGTCGGACGTGCGGCGC
CCGCTGGGGGTGCTGCTCTTCGTGGGCCCCACCGGCGTGGGCAAGACGGAGCTGTCCAAGGCGCTGGCGGAGCTACTCTT
CGGTGCGAAGGAGCGCATGGTCCGCCTGGACATGGGTGAGTACGCCGGTCCGGACGCCCTGCTGCGGTTGATGGGAGACG
GGGAGACGCCGGGCTACCTCACCTCGGCGGTGCGCCGGCAGCCCTTCTGCGTGGTGCTGCTGGACGAGGTGGAGAAGGCC
CACCCCGCCGTTCACGATGCGCTGCTGGGCGTCTTGGGCGAAGGCCGCCTCACCGATGCCTCCGGGCGCTTCACGGACTT
CCGCAACGCCCTCATCATCCTCACCAGCAACCTGGGCGCGGACACCCTGCGCGCCCGCGTGGGCTTCGATGCGTCGGGCG
GTGCCCCGGACATGGCCTCCCTGCGCGCGCACTACCTCTCCGAGGTGCAGCGCTTCTTCCGGCCGGAGATGTTCAACCGC
ATGGACGACGTGGTCGTCTTCTCGCCGCTGTCCGCCCCGCTGCTGCGCCGGTTGGTGGTCCGCGAGCTTGAAGCCGTATG
CCGCCGTCCAGGCCTCTCGCTCCACGATGCCCTGCTGGAGGTCACCCCCGCCGCCCAGGACTGGCTGGCCCTGAGAGGCT
TCGACGCGCGGTATGGTGCCCGCCCCTTGAAGCGCGCGCTGGAGCGCGAGCTGGTGGTCCCCGTGGCGGACTGGCTCGCG
GCGCATCCCCGCATGGGGCCGGCGAACCTCACGGTGGACGCGGGAACCACGGGACTCGAACTGCGCGCCGAGTCCCTGGG
CGGCGCCGCCGAGGGCGGGGGCCGGGAAGCCATCGAGCGGATCCTCGAGGAAGCAGCCACCGTGCGCGCGGAAGTCCAGC
GTTGGAGCCGCTGCGCGCCCATGCACTCCCTGCGCCGCGTCCTGGCCGTCTTCGACAAGGTCTCCCGGCACACCGCGTAC
TGGGAGGAGCGGGCGCTGGCCGAAGAAAACTCGCGCAAGTCATCGGAGGCGCGAGCGCTGGAACAGTCGCTTCGGGAGTG
CGCCCAGCAGGCGGAGGCCATCGAGGACCTGCTCTTCGAGGCCCACCTGTCCAGGACCGTGGATCAGGTGGAGTCCCTGA
GCCGCGAGGTGAGCCAGGTGAAGGCGGCCTTCGCGCGCATCCGGCAACGCATCTACTCCTCCCTCTTTCCCCCGTCACAT
GGCGCGACCCTCTACCTGGTGCCAGGTCGCGGCGCGTGGAGGCAGGCACGCATGCTCGCCGCGGCCTATGAGGGGTGGGC
CCAGAAGGCGTCCATGGAGAAGCGGCGAGGCCTGCTCGCCGTCCCCGAGCGCAAGTCCGGCGAGAAGGCCCATCGCACCC
CTCCGCCGCCGCAATGGCGGTGGGCCGACGATGAGACCTTCGACAAGCTCGTCGTGCAGCCGGTCGCCTATGCGATTCAG
GTCAAGGGCGGCCCCCACGCCCTGCTGCTCGCTGGCGAGCACGGCCTGCATCGCTTCATCGAAGGCAGTCAGATTTCGAT
GGTGCATGCGTACTTCGAGCCGCGGCCCCTCTCGCTGTTCGACCTCTCCTCCCGGGAGGCGTTGCAGAAGCAGTTGCCGA
AAAACGAGGTCCGTCAGGTGCGCCTGTCGGGCAGCGGTTCCGGCCAGGGGACGCTGACGGACCTTCGCACCGGGGTGCAG
CAGCGCTTCGGTCTGGAGGGGCCTGACCTGGAGCCCCTGCTGGAGCCCTGGATGCAGTGGCGCGTCTTCAACAGCCGGGA
CGAGGACTGA					