SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00302
Cognate effector	EFF01476
Related T6SS (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (384 a.a.); Nucleotide sequence (1152 bp)
Description	TsxEI comprise a T6SS-dependent effector-immunity pair.
Reference	[1] Troselj V, Treuner-Lange A, Søgaard-Andersen L, et al. Physiological Heterogeneity Triggers Sibling Conflict Mediated by the Type VI Secretion System in an Aggregative Multicellular Bacterium. mBio. 2018 Feb 6;9(1). pii: e01645-17. doi: 10.1128/mBio.01645-17. PMID: 29437919

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	MXAN_5571 (TsxI)
Coordinates (Strand)	6919379..6920530 (-)
NCBI ID	108762153
RefSeq	NC_008095
Uniprot ID	UPI0000D84EE3
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-383	MEPNKDPFLLTPQLPAAAAPVRHPSPFPWNQVALVFPSPAPDSVSVLTLNDKLWRLELSGSEVRRVEVDDEYLEQVSGGELQGMQPPSPTHLAYLQSRSLVMYDLKWQLAKTWFMVERLEEVAIRGAWISHAPLVVAAELEDTTNYARTSRISFRLRTWRLEGEKRAKLGSLELGSVVGAVKWDAGAGLVAVQKPGVPLELYGPDLARPQPEHPLALALRKLTVDGLTLQSLRLHPSLPVALVALGRDVTPKREEGKEKPEDPGIWRVSWEGAPAVTVRLARYASGESVTLGSLSPEGDWLYYRVLNSAGRNPQLYVQQVGAAFKPPLALGAVPEKGAALLWTGGAPSLVMYDGTKDALVHWKLAGAAAPAKPPAAPPKPDGK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9202	-	-

Protein sequence: 384 a.a. .

                                    >IMU00302 NC_008095:c6920530-6919379 [Myxococcus xanthus DK 1622]
MEPNKDPFLLTPQLPAAAAPVRHPSPFPWNQVALVFPSPAPDSVSVLTLNDKLWRLELSGSEVRRVEVDDEYLEQVSGGE
LQGMQPPSPTHLAYLQSRSLVMYDLKWQLAKTWFMVERLEEVAIRGAWISHAPLVVAAELEDTTNYARTSRISFRLRTWR
LEGEKRAKLGSLELGSVVGAVKWDAGAGLVAVQKPGVPLELYGPDLARPQPEHPLALALRKLTVDGLTLQSLRLHPSLPV
ALVALGRDVTPKREEGKEKPEDPGIWRVSWEGAPAVTVRLARYASGESVTLGSLSPEGDWLYYRVLNSAGRNPQLYVQQV
GAAFKPPLALGAVPEKGAALLWTGGAPSLVMYDGTKDALVHWKLAGAAAPAKPPAAPPKPDGK*

                                

Nucleotide sequence: 1152 bp .

                                    >IMU00302 NC_008095:c6920530-6919379 [Myxococcus xanthus DK 1622]
ATGGAGCCCAACAAGGACCCCTTCCTGCTGACGCCTCAACTGCCCGCGGCCGCGGCGCCCGTGCGTCATCCCTCACCGTT
CCCCTGGAATCAGGTGGCGCTCGTGTTTCCATCACCGGCGCCGGATTCGGTGTCGGTGCTCACGCTGAATGACAAGCTCT
GGCGGCTGGAGCTGTCCGGCTCCGAGGTGCGCCGCGTCGAGGTGGATGACGAGTACCTGGAGCAGGTCTCCGGAGGCGAG
CTGCAAGGGATGCAGCCTCCGTCACCCACGCACCTGGCGTACCTCCAGAGTCGAAGCCTGGTGATGTACGACCTCAAGTG
GCAACTGGCCAAGACGTGGTTCATGGTCGAGCGACTGGAGGAGGTCGCCATCCGCGGCGCGTGGATTTCTCACGCCCCGC
TGGTGGTGGCGGCGGAGCTGGAGGACACCACGAATTATGCCCGCACCTCCCGCATCTCCTTCCGGCTGCGCACCTGGCGG
CTGGAGGGTGAAAAGCGCGCGAAGTTGGGCTCGCTGGAGCTGGGCTCCGTCGTCGGCGCGGTGAAGTGGGACGCGGGGGC
GGGGCTGGTCGCGGTGCAGAAGCCCGGCGTGCCGCTGGAGCTCTACGGGCCTGACCTGGCCAGGCCCCAGCCGGAGCACC
CGCTGGCGCTCGCGCTGCGCAAGCTGACGGTGGATGGCCTCACGTTGCAGTCGCTGCGTTTGCATCCCTCGTTGCCGGTG
GCGCTCGTGGCTCTGGGCCGTGACGTGACGCCGAAGCGGGAGGAGGGGAAGGAGAAGCCCGAGGACCCTGGCATCTGGCG
CGTGTCGTGGGAAGGCGCGCCCGCGGTGACGGTGCGGCTAGCGCGCTATGCCTCCGGGGAGTCGGTGACGCTGGGCTCGT
TGTCGCCCGAGGGTGACTGGCTCTACTACCGCGTCCTGAACAGCGCGGGCCGCAACCCGCAGCTCTACGTGCAGCAGGTG
GGCGCGGCCTTCAAGCCGCCCCTGGCGCTGGGCGCTGTTCCGGAGAAGGGCGCGGCGCTGCTGTGGACGGGCGGGGCTCC
GTCGCTCGTCATGTATGACGGGACGAAGGACGCGCTGGTTCACTGGAAGCTGGCGGGCGCGGCGGCACCGGCGAAGCCTC
CCGCGGCGCCGCCCAAGCCAGACGGGAAGTGA

                                