SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001241
Component protein type	TssH
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (1128 a.a.); Nucleotide sequence (3384 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23445
Coordinate (Strand)	6035918..6039301 (+)
NCBI ID	WP_011554810.1
RefSeq	NC_008095
Uniprot ID	Q1D2Y8_MYXXD, A0A7Y4HET3_MYXXA
KEGG ID	mxa:MXAN_4824
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	6e-33	533..691
ClpB_D2-small	PF10431.11	3.8e-13	698..775
AAA	PF00004.31	1.2e-06	258..352

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1127	MDLKLPFVVAPHSGRLVEAWVPAFFPALHKVGPSLSMLRDELALAVMERFERESPSQVAQYQLPPHFALKRVTVDAEGRDREKNRRVVLQGTMSVLLEKWPRDTFWVVTPTRLPAARFALNQPADLPQALARRLMAWCLEHNLDSLDTHWAQGREQLELLEVDAYAPSVLPRTPPRPAQPARRRRTKNPEKEDTAPETPEQREQRRNRRRLTLVELRAVARNLSHGARDGTLERCFGREALVRELVEALEGREGSALVLVGPPGAGKTALIHEAVSRLTARQDAAGVRRDVWRVDGNQFIAGMMYVGQWEARARGVVKELMEVGDLLYVDDLASLVYAGRTRNERTNVAQFLEPHMARGELTVLAESTPERFERVREEAPTLASLFRVIHVPALDARATLPALLGTVRDLEAEGTDSAVQLSPLALETLLSLQERFVSHEAFPGKAVRLLRRVMARQGVSTSGTRRFSSGDVIDAMREQTGLPDFILGSAQPKKREALTWEMQRQVAGQPEAVEAVVDAILTLQSALQPSDKPLATYLFVGPTGVGKTETAKALARTLFGGEQRLIRFDMSEFVTSSSITRLLGRPGAPDGELTTALRTQPFCVVLFDEVEKAHPRVFDALLQFLGEGRLTDGAGRTVDARQSVVVLTSNLGVREAATHTGFHRAADSADAHYLSAVRAWFRPEFFNRLDRVVPFRPLTPAALRVVVDLALEALLSRHGIRQGNVLVEVEPRLLDLLVEEAYDPRYGARPLKRALEKRLTLPLAHHLVRRSADDLARVELLRDGNDMRVSVEVLVNAPAWAPERPASAWSLTEVSRLLEETRSRLEALMTEGVPEAGELAERLHRLALEATDIRDYELVPRDFLDAESPASTVKQMLTSHSSHVGHLGLRQRPVYEERPLHVSNEEQLRRAQPRVLALRDETAWVEHQLTHLARGTDVVTVLVEGLGDATPSAVWTVAHSLPSALGESAMYLEAVHADGRTEWLRSGDERSEDLVLRRVAVRMSAIGLREVLAPLRGYALVDLVYGDGPRQVLVRLEHLSEDVGSLEDVPRVMAAWDEAVVRERDARRSGAASVGAASHVILRGREGVTTGRPHDSTELTHVASGRRPREALAWAARVLREPSQGHD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9724	-	-

Protein sequence: 1128 a.a.

						>T6CP001241 NC_008095:6035918-6039301 [Myxococcus xanthus DK 1622] [TssH]
MDLKLPFVVAPHSGRLVEAWVPAFFPALHKVGPSLSMLRDELALAVMERFERESPSQVAQYQLPPHFALKRVTVDAEGRD
REKNRRVVLQGTMSVLLEKWPRDTFWVVTPTRLPAARFALNQPADLPQALARRLMAWCLEHNLDSLDTHWAQGREQLELL
EVDAYAPSVLPRTPPRPAQPARRRRTKNPEKEDTAPETPEQREQRRNRRRLTLVELRAVARNLSHGARDGTLERCFGREA
LVRELVEALEGREGSALVLVGPPGAGKTALIHEAVSRLTARQDAAGVRRDVWRVDGNQFIAGMMYVGQWEARARGVVKEL
MEVGDLLYVDDLASLVYAGRTRNERTNVAQFLEPHMARGELTVLAESTPERFERVREEAPTLASLFRVIHVPALDARATL
PALLGTVRDLEAEGTDSAVQLSPLALETLLSLQERFVSHEAFPGKAVRLLRRVMARQGVSTSGTRRFSSGDVIDAMREQT
GLPDFILGSAQPKKREALTWEMQRQVAGQPEAVEAVVDAILTLQSALQPSDKPLATYLFVGPTGVGKTETAKALARTLFG
GEQRLIRFDMSEFVTSSSITRLLGRPGAPDGELTTALRTQPFCVVLFDEVEKAHPRVFDALLQFLGEGRLTDGAGRTVDA
RQSVVVLTSNLGVREAATHTGFHRAADSADAHYLSAVRAWFRPEFFNRLDRVVPFRPLTPAALRVVVDLALEALLSRHGI
RQGNVLVEVEPRLLDLLVEEAYDPRYGARPLKRALEKRLTLPLAHHLVRRSADDLARVELLRDGNDMRVSVEVLVNAPAW
APERPASAWSLTEVSRLLEETRSRLEALMTEGVPEAGELAERLHRLALEATDIRDYELVPRDFLDAESPASTVKQMLTSH
SSHVGHLGLRQRPVYEERPLHVSNEEQLRRAQPRVLALRDETAWVEHQLTHLARGTDVVTVLVEGLGDATPSAVWTVAHS
LPSALGESAMYLEAVHADGRTEWLRSGDERSEDLVLRRVAVRMSAIGLREVLAPLRGYALVDLVYGDGPRQVLVRLEHLS
EDVGSLEDVPRVMAAWDEAVVRERDARRSGAASVGAASHVILRGREGVTTGRPHDSTELTHVASGRRPREALAWAARVLR
EPSQGHD*					

Nucleotide sequence: 3384 bp

						>T6CP001241 NC_008095:6035918-6039301 [Myxococcus xanthus DK 1622] [TssH]
ATGGACCTGAAGCTTCCCTTCGTGGTGGCCCCGCACAGCGGCCGGCTCGTCGAGGCCTGGGTGCCCGCCTTCTTCCCGGC
GCTGCACAAGGTGGGGCCCAGCCTCTCCATGCTGCGAGACGAACTCGCGCTCGCCGTCATGGAGCGCTTCGAGCGCGAAT
CGCCCTCCCAGGTGGCGCAGTACCAACTTCCACCCCACTTCGCCCTCAAGCGCGTCACCGTCGATGCGGAAGGAAGGGAC
CGGGAGAAGAACCGGCGCGTCGTCCTCCAGGGCACCATGTCCGTGCTGCTGGAGAAGTGGCCCCGTGACACCTTCTGGGT
CGTCACCCCCACCCGGCTCCCCGCCGCGCGCTTCGCGCTGAACCAGCCCGCGGACCTGCCCCAGGCGCTCGCGCGACGGC
TGATGGCCTGGTGTCTGGAACACAACCTGGACTCCCTGGACACGCACTGGGCCCAGGGCCGGGAGCAACTGGAGCTCCTG
GAGGTGGATGCCTATGCCCCCTCCGTCCTCCCGCGCACGCCGCCGCGACCGGCCCAGCCCGCGCGACGCCGCCGGACCAA
GAATCCGGAGAAGGAAGACACCGCGCCCGAAACGCCAGAGCAGCGGGAGCAACGCCGCAACCGGCGGCGCCTCACCCTGG
TGGAGCTCCGGGCCGTGGCGCGCAACCTGAGCCACGGTGCGCGAGACGGCACGCTGGAACGTTGCTTCGGCCGGGAGGCG
CTGGTTCGCGAGCTCGTAGAGGCGCTCGAAGGCCGCGAAGGCTCCGCGCTGGTGCTGGTGGGTCCACCTGGCGCGGGCAA
GACGGCCCTGATTCATGAAGCGGTGAGCCGGCTCACCGCCCGGCAGGACGCCGCGGGCGTGCGTCGCGACGTGTGGCGCG
TGGACGGCAACCAGTTCATCGCCGGGATGATGTACGTGGGCCAGTGGGAGGCCCGCGCGCGGGGCGTGGTGAAGGAGCTG
ATGGAGGTCGGCGACCTGCTCTACGTGGACGACCTGGCCTCGCTCGTCTACGCGGGCCGCACGCGCAATGAGCGCACCAA
CGTCGCGCAGTTCCTGGAGCCCCACATGGCCCGGGGCGAGCTCACCGTGCTCGCCGAGTCCACGCCCGAGCGCTTCGAGC
GCGTGCGGGAAGAAGCCCCCACCCTCGCCTCGCTCTTCCGGGTCATCCATGTCCCCGCGCTGGATGCGCGTGCCACGCTG
CCCGCGCTCCTCGGCACCGTGCGCGACCTGGAGGCGGAAGGCACGGACAGCGCGGTCCAGCTCTCGCCGCTGGCGCTGGA
GACCCTGCTCTCATTGCAGGAGCGCTTCGTGTCGCACGAGGCGTTCCCGGGCAAGGCCGTCCGTCTGCTCCGCCGCGTCA
TGGCCCGACAGGGCGTCAGCACCTCGGGCACCCGGCGCTTTTCGTCGGGGGACGTCATCGACGCGATGCGGGAGCAGACG
GGCCTACCGGATTTCATCCTGGGAAGCGCCCAGCCGAAGAAGCGCGAAGCGCTGACGTGGGAGATGCAGCGGCAGGTAGC
GGGCCAGCCCGAAGCCGTGGAAGCCGTGGTGGACGCCATCCTCACGTTGCAGTCCGCGCTCCAGCCGTCCGACAAGCCCC
TGGCCACCTACCTCTTCGTGGGCCCCACGGGCGTGGGCAAGACGGAGACGGCGAAGGCGCTCGCGCGGACGCTCTTCGGC
GGAGAGCAGCGGCTCATCCGTTTCGACATGTCGGAGTTCGTCACGTCCTCCAGCATCACCCGGCTGCTCGGGCGGCCCGG
CGCGCCCGACGGTGAGCTGACCACCGCGCTGCGCACCCAGCCCTTCTGCGTGGTGCTGTTTGACGAGGTGGAGAAGGCCC
ATCCCCGCGTGTTCGACGCCCTCCTCCAGTTCCTTGGTGAGGGACGGCTGACGGACGGAGCGGGACGCACGGTGGATGCC
CGGCAGTCCGTCGTCGTGCTCACGAGCAACCTCGGCGTGCGCGAGGCCGCCACCCACACCGGTTTCCACCGCGCCGCGGA
CAGCGCGGATGCGCACTACCTCTCCGCCGTGCGTGCCTGGTTCCGCCCGGAGTTCTTCAACCGCCTGGACCGGGTGGTGC
CCTTCCGTCCGCTCACGCCCGCCGCGCTGCGCGTGGTGGTGGACCTCGCGCTCGAAGCCCTGCTGTCGCGCCATGGCATC
CGGCAGGGCAACGTGCTGGTGGAGGTCGAGCCGCGCCTGCTGGACCTGCTGGTGGAGGAGGCCTACGACCCGCGCTACGG
AGCCCGCCCCCTCAAGCGCGCACTGGAGAAGCGCCTCACGTTGCCCCTGGCCCACCACCTGGTGCGGCGGAGCGCGGACG
ACCTGGCGCGCGTGGAGCTGCTACGGGACGGCAACGACATGCGCGTGTCGGTGGAGGTGCTGGTCAACGCGCCCGCGTGG
GCGCCCGAGCGGCCGGCCTCTGCGTGGAGCCTGACCGAGGTTTCCCGCCTGCTCGAGGAGACCCGCTCGCGGCTGGAGGC
GTTGATGACGGAGGGGGTGCCCGAGGCGGGAGAACTGGCGGAGCGACTGCACCGGCTGGCACTCGAGGCCACGGACATTC
GCGACTACGAGTTGGTCCCGCGCGACTTCCTGGACGCGGAGTCGCCTGCGTCCACCGTGAAGCAGATGCTGACGTCGCAC
TCGAGTCACGTGGGCCACCTGGGCCTGCGGCAGCGCCCTGTGTATGAGGAGCGCCCACTGCACGTCTCGAACGAAGAACA
GCTCCGCCGCGCACAGCCCCGGGTGTTGGCCCTTCGCGACGAGACCGCCTGGGTCGAACATCAGCTCACGCACCTGGCGA
GGGGGACGGACGTCGTGACGGTGTTGGTGGAGGGGCTCGGCGACGCGACGCCATCCGCGGTGTGGACCGTGGCCCACTCC
CTCCCCAGCGCGCTCGGGGAGTCGGCGATGTACCTGGAAGCCGTCCATGCCGACGGGCGCACCGAGTGGCTCCGGTCCGG
AGACGAGCGGTCCGAGGACCTGGTGCTTCGGCGTGTCGCCGTGCGGATGTCGGCCATCGGCCTGCGCGAGGTGCTGGCGC
CCCTTCGAGGCTATGCGCTGGTGGACCTCGTCTATGGCGATGGGCCACGCCAGGTCCTGGTCCGGCTGGAGCACCTGTCC
GAAGACGTCGGGAGTCTGGAAGACGTGCCGCGCGTGATGGCCGCATGGGATGAGGCAGTCGTCCGCGAACGCGATGCCCG
GAGGAGCGGCGCCGCGTCAGTGGGCGCGGCCAGCCACGTCATCCTGCGTGGCCGCGAAGGTGTCACGACTGGGCGGCCCC
ACGACTCGACCGAACTCACGCACGTCGCGAGCGGACGCCGCCCCCGGGAGGCGCTCGCATGGGCCGCGAGGGTGCTCCGG
GAACCCAGCCAGGGACACGACTGA					