SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009587
Component protein type	TssA
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (675 a.a.); Nucleotide sequence (2025 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23360
Coordinate (Strand)	6013836..6015860 (+)
NCBI ID	WP_011554792.1
RefSeq	NC_008095
Uniprot ID	Q1D306_MYXXD, A0A7Y4M6Z0_MYXXA
KEGG ID	mxa:MXAN_4806
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasJ	PF16989.7	1.3e-52	370..631
ImpA_N	PF06812.14	1.4e-22	17..128

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-674	MPPSLEQLRERALPWAEPVPGASPAGVQAKHEPAYEAVALEVAKLESPASNAVRWDDVVEGASELLKHTTKDLWLASYMAYGLYATRGLDGAATGTAVLAEVTERYWQDLFPEAKRLRGRANAVAWFVDRLGRILPTVDQASVSAEPLDALAVAVKRLAQLSRERFSDMAPAFGPLQDAIARLRAGLPEPEPESVPDGRTASSEDADASQPPVDGTASSQDEDTPSGEDGETVVRAADSANGTRPGAPSDSAETPTNGNGNRSAREAGNASANGKPAQANGAGNSAAPGKGNTAPGNPTAKNAPAPAKISTAQGTAKASAASQGGASANGTPAPPRAAIEVPPLPELPAAPDLSSVEAITDFLRTVGGALLGAAGALRRASPEDPLSYRLNRMGLWLHLTQPPATDGNGRTQIHPLPDLLGTKLETLEQNQRWGELLDESESALARHRFTLTLHRYSATALEGLGAAYAGARTALVQELAIQLRRMTGVETLISTLGSPLTDDATQDWLRDHVLRASAPPPAPSAAVASVSTTLALGPLSLGPSVHADSAALEAEARTLLEEGRVHEAVTRLQAAVAAASTGRARFISRLELARLCANAGQLPLARAVYDALDEEVSAHALDTWEPALAAACLEGWLQTRTEAEKESGPVAVKFRNRYRRLALLDSSATLRVGA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9099	-	-

Protein sequence: 675 a.a.

						>T6CP009587 NC_008095:6013836-6015860 [Myxococcus xanthus DK 1622] [TssA]
MPPSLEQLRERALPWAEPVPGASPAGVQAKHEPAYEAVALEVAKLESPASNAVRWDDVVEGASELLKHTTKDLWLASYMA
YGLYATRGLDGAATGTAVLAEVTERYWQDLFPEAKRLRGRANAVAWFVDRLGRILPTVDQASVSAEPLDALAVAVKRLAQ
LSRERFSDMAPAFGPLQDAIARLRAGLPEPEPESVPDGRTASSEDADASQPPVDGTASSQDEDTPSGEDGETVVRAADSA
NGTRPGAPSDSAETPTNGNGNRSAREAGNASANGKPAQANGAGNSAAPGKGNTAPGNPTAKNAPAPAKISTAQGTAKASA
ASQGGASANGTPAPPRAAIEVPPLPELPAAPDLSSVEAITDFLRTVGGALLGAAGALRRASPEDPLSYRLNRMGLWLHLT
QPPATDGNGRTQIHPLPDLLGTKLETLEQNQRWGELLDESESALARHRFTLTLHRYSATALEGLGAAYAGARTALVQELA
IQLRRMTGVETLISTLGSPLTDDATQDWLRDHVLRASAPPPAPSAAVASVSTTLALGPLSLGPSVHADSAALEAEARTLL
EEGRVHEAVTRLQAAVAAASTGRARFISRLELARLCANAGQLPLARAVYDALDEEVSAHALDTWEPALAAACLEGWLQTR
TEAEKESGPVAVKFRNRYRRLALLDSSATLRVGA*					

Nucleotide sequence: 2025 bp

						>T6CP009587 NC_008095:6013836-6015860 [Myxococcus xanthus DK 1622] [TssA]
ATGCCTCCCTCCCTGGAACAGCTTCGTGAACGTGCCCTCCCCTGGGCCGAGCCCGTGCCGGGGGCGTCCCCGGCCGGCGT
GCAGGCGAAGCACGAGCCCGCATACGAGGCCGTCGCGCTGGAAGTCGCGAAGTTGGAGTCTCCGGCCAGCAACGCCGTGC
GCTGGGATGACGTCGTCGAGGGCGCCAGCGAGCTGCTGAAGCACACCACCAAGGACCTCTGGCTCGCCTCGTACATGGCC
TATGGCCTGTATGCCACGCGAGGCCTGGACGGCGCGGCCACCGGCACGGCCGTGCTCGCCGAGGTGACGGAGCGCTACTG
GCAGGACCTCTTCCCGGAGGCGAAGCGGCTGCGCGGCCGAGCCAACGCCGTGGCCTGGTTCGTGGACCGGCTGGGCCGCA
TCCTGCCCACCGTAGACCAGGCCTCCGTGAGCGCGGAGCCCCTGGATGCGCTGGCGGTCGCGGTGAAGCGCCTGGCCCAG
CTCTCACGCGAGCGGTTCTCCGACATGGCCCCCGCGTTCGGGCCGCTCCAGGATGCCATCGCCCGGCTCCGCGCCGGACT
CCCCGAGCCCGAACCCGAGAGCGTACCGGATGGACGCACCGCGTCCTCGGAGGACGCAGACGCCTCGCAGCCGCCGGTGG
ATGGGACTGCGTCGTCCCAGGACGAGGACACCCCGTCCGGCGAGGATGGAGAGACCGTTGTGCGCGCGGCGGACTCCGCG
AATGGCACGCGGCCAGGAGCGCCTTCGGACAGCGCTGAAACGCCCACGAACGGCAATGGCAACCGCTCCGCTCGGGAGGC
GGGCAATGCCAGCGCCAACGGCAAACCTGCTCAAGCCAACGGTGCTGGAAACAGCGCCGCCCCGGGTAAAGGCAACACCG
CCCCGGGCAATCCCACGGCGAAGAACGCCCCCGCCCCGGCCAAAATCAGCACCGCTCAGGGCACCGCCAAAGCCAGCGCA
GCCAGCCAGGGCGGCGCCTCCGCCAACGGCACACCCGCACCACCGCGCGCCGCCATCGAAGTCCCGCCCCTCCCCGAGCT
TCCCGCCGCCCCGGATCTGTCCAGCGTGGAGGCGATCACCGACTTCCTGCGCACCGTGGGCGGCGCGCTGCTGGGAGCCG
CGGGCGCGCTGCGCCGGGCCAGCCCCGAGGATCCGCTCTCCTACCGCCTCAACCGCATGGGCCTGTGGTTGCACCTGACC
CAGCCGCCCGCCACGGATGGCAATGGCCGCACCCAGATTCACCCACTGCCGGACCTGCTGGGAACCAAGCTGGAGACCCT
GGAGCAGAACCAGCGCTGGGGCGAGCTGCTGGACGAGTCCGAGTCGGCCCTGGCCCGCCACCGCTTCACGCTGACGCTCC
ACCGGTACAGCGCCACCGCCCTGGAGGGGCTGGGTGCCGCGTACGCCGGGGCTCGCACCGCGCTGGTACAGGAATTGGCC
ATCCAGCTCCGCCGCATGACCGGCGTGGAGACGCTCATCTCCACCTTGGGAAGCCCGCTCACGGACGACGCCACCCAGGA
CTGGCTGCGCGACCACGTCCTCCGCGCGAGCGCGCCCCCACCCGCCCCTTCCGCCGCCGTGGCGTCGGTGTCCACGACCC
TGGCGCTGGGGCCCCTGTCGCTGGGCCCGTCTGTCCATGCCGACAGCGCGGCGCTGGAAGCCGAAGCCCGCACGTTGCTG
GAGGAAGGCCGTGTCCACGAAGCTGTCACCCGGTTGCAGGCCGCCGTCGCCGCCGCCAGCACCGGCCGCGCCCGCTTCAT
CTCCCGGCTGGAGCTGGCGCGGTTGTGTGCCAATGCGGGCCAGTTGCCGCTCGCGCGCGCCGTCTATGACGCGCTGGACG
AAGAGGTCTCCGCCCACGCGCTCGACACCTGGGAGCCAGCGCTCGCCGCCGCGTGTCTGGAGGGGTGGCTGCAAACCCGC
ACCGAAGCCGAAAAGGAGTCTGGACCGGTGGCAGTGAAATTTCGAAACCGGTATCGTCGTCTAGCGTTGCTGGACTCTTC
CGCCACGCTGCGCGTCGGCGCTTGA					