SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001237
Component protein type	TssF
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (584 a.a.); Nucleotide sequence (1752 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23385
Coordinate (Strand)	6019075..6020826 (+)
NCBI ID	WP_011554797.1
RefSeq	NC_008095
Uniprot ID	Q1D301_MYXXD, A0A7Y4HEV4_MYXXA
KEGG ID	mxa:MXAN_4811
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.4e-152	2..580

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-583	MFSKYYLSELSYLREMGRAFGLANPSVAGLLVERGADPDVERLLEGFAFLAARIRERVDDDVPELVHGLTELLLPHYLRPLPASTIVEFTPHLRALRGRSRLAAGAEVASQPIDGTSCVFRTTTDVDLLPVQLTDALLDRSSITAPVLRLYFQTTEQGRAEVLREQGLRLFIHADLSAASVVLLWLLRYCRQVRVRGASGQGDGIKLPVNAIQPVGFHRDFKLLPWPRASEGYRHLQEYLTLPEKFLFFEVRGLDAAAGLKEDRFEIAFHLERPPPLDARIHREMFRLHCAPVVNLFSVPADPILHRTLDREHLLRASDLPPNHAEIYSVDSVTGLKAGRNERRVYRPFYEFTHTAGGDAEQSFYRLRRAPSPLDEGIDTYITLETPRNIAPEVNAEEALSMDLTCTNRSLPSRLQVGDLTASTSASPTQAKFKNISPVSRPARAPLGTELHWRLLSHLAINQHSLADAAALRRLMDLYNFHSLTDNLAARASRLRINAIRAVETKPVTRFLEGAPLRGHRTRVDLDEENFMGVGDSFLFGSVLEELLASHVTINSFNELSIRLHPSQTEFAWLPRNGSQTLL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9396	-	-

Protein sequence: 584 a.a.

						>T6CP001237 NC_008095:6019075-6020826 [Myxococcus xanthus DK 1622] [TssF]
MFSKYYLSELSYLREMGRAFGLANPSVAGLLVERGADPDVERLLEGFAFLAARIRERVDDDVPELVHGLTELLLPHYLRP
LPASTIVEFTPHLRALRGRSRLAAGAEVASQPIDGTSCVFRTTTDVDLLPVQLTDALLDRSSITAPVLRLYFQTTEQGRA
EVLREQGLRLFIHADLSAASVVLLWLLRYCRQVRVRGASGQGDGIKLPVNAIQPVGFHRDFKLLPWPRASEGYRHLQEYL
TLPEKFLFFEVRGLDAAAGLKEDRFEIAFHLERPPPLDARIHREMFRLHCAPVVNLFSVPADPILHRTLDREHLLRASDL
PPNHAEIYSVDSVTGLKAGRNERRVYRPFYEFTHTAGGDAEQSFYRLRRAPSPLDEGIDTYITLETPRNIAPEVNAEEAL
SMDLTCTNRSLPSRLQVGDLTASTSASPTQAKFKNISPVSRPARAPLGTELHWRLLSHLAINQHSLADAAALRRLMDLYN
FHSLTDNLAARASRLRINAIRAVETKPVTRFLEGAPLRGHRTRVDLDEENFMGVGDSFLFGSVLEELLASHVTINSFNEL
SIRLHPSQTEFAWLPRNGSQTLL*					

Nucleotide sequence: 1752 bp

						>T6CP001237 NC_008095:6019075-6020826 [Myxococcus xanthus DK 1622] [TssF]
ATGTTCAGCAAGTATTACCTGAGCGAGCTGTCGTACCTGCGGGAGATGGGACGGGCCTTCGGGCTGGCCAACCCCTCTGT
CGCGGGCCTGTTGGTGGAGCGCGGCGCGGACCCGGACGTGGAGCGGCTGCTGGAGGGCTTCGCGTTCCTCGCGGCCCGCA
TCCGCGAGCGCGTGGACGACGACGTGCCGGAGCTGGTGCACGGGCTCACCGAGCTGCTGCTACCGCACTACCTGCGCCCG
CTGCCGGCCTCCACCATCGTCGAGTTCACCCCGCACCTGCGCGCGCTGCGCGGCCGCTCGCGGCTGGCCGCCGGGGCGGA
GGTGGCGTCGCAGCCGATTGACGGCACCAGTTGCGTCTTCCGCACCACCACGGACGTGGACCTGCTGCCGGTGCAGCTCA
CCGACGCACTCCTGGACCGGTCGTCCATCACCGCGCCGGTGCTGCGCCTCTATTTCCAGACGACGGAGCAGGGCCGCGCG
GAGGTGTTGCGCGAGCAAGGCCTGCGCCTGTTCATCCACGCCGACCTGTCCGCTGCGTCGGTGGTGCTGCTGTGGTTGCT
CCGCTACTGCCGCCAGGTGCGCGTGCGCGGCGCGTCTGGCCAGGGAGACGGCATCAAGCTGCCGGTGAATGCCATCCAAC
CGGTGGGCTTCCACCGGGACTTCAAGCTGCTGCCCTGGCCGCGCGCCAGCGAGGGCTACCGGCACCTCCAGGAGTACCTG
ACGCTGCCGGAGAAGTTCCTGTTCTTCGAGGTGCGCGGGCTGGACGCCGCCGCCGGGCTGAAAGAGGACCGCTTCGAGAT
TGCCTTCCACCTGGAGCGGCCGCCGCCCCTGGATGCGCGCATCCACCGGGAGATGTTCCGGCTACATTGCGCCCCCGTGG
TGAACCTCTTCAGCGTGCCGGCGGACCCCATCCTCCACCGCACGCTGGACCGGGAGCACCTGCTGCGCGCGTCCGACCTG
CCCCCCAACCACGCGGAGATTTACTCGGTGGACTCGGTGACGGGCCTGAAGGCCGGACGCAACGAGCGGCGCGTCTACCG
GCCCTTCTACGAGTTCACCCACACCGCGGGCGGGGACGCCGAGCAGTCCTTCTATCGGCTGCGCCGGGCGCCTTCGCCGC
TGGACGAAGGAATCGACACGTACATTACGCTGGAGACGCCGCGGAACATCGCGCCGGAGGTCAACGCCGAGGAAGCGCTG
TCCATGGACCTGACGTGCACCAACCGCTCGCTGCCCTCGCGGCTGCAGGTGGGTGACTTGACGGCATCCACCTCCGCCAG
CCCCACGCAGGCGAAGTTCAAGAACATCTCCCCGGTGAGCCGGCCCGCGCGCGCGCCGCTGGGCACCGAACTGCACTGGC
GGCTCCTGTCGCACCTGGCCATCAACCAGCACTCGCTGGCGGACGCGGCCGCACTGCGGCGGCTGATGGACCTGTACAAC
TTCCATTCGCTCACCGACAACCTGGCGGCGCGCGCCAGCCGCCTGCGCATCAACGCCATCCGCGCGGTGGAGACGAAGCC
GGTGACGCGCTTCCTGGAGGGCGCGCCGCTGCGGGGTCACCGCACCCGCGTGGACCTGGACGAAGAGAACTTCATGGGCG
TGGGCGATTCGTTCCTCTTCGGCTCCGTGCTGGAGGAACTGCTCGCCTCCCACGTCACCATCAACTCCTTCAACGAGCTG
AGCATCCGGCTTCACCCCTCGCAGACGGAGTTCGCGTGGCTTCCGAGGAACGGCTCCCAGACGCTTTTGTAG					