SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001233
Component protein type	TssL
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (239 a.a.); Nucleotide sequence (717 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23345
Coordinate (Strand)	6008478..6009194 (+)
NCBI ID	WP_011554789.1
RefSeq	NC_008095
Uniprot ID	Q1D309_MYXXD, A0A7Y4M7B4_MYXXA
KEGG ID	mxa:MXAN_4803
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	6.8e-39	6..203

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-182	MDRVTEATKDCFDAAIQLRGSEASAVPPPETLHHRLRGVVDETLRRAAVLGFSHQDAQDMAYALVALIDEVVLGRPEEYRQLWMPLQLHYFNENVAGDGFFARLNTVRKDPHRHEVLQVYYLCMLFGFQGRYRIRGGELELMTLIDTVQKDLERARPFDFDVLSPHGDRPTESLLSKRKKAS
Transmembrane helix	183-205	MVSISAGALAVAVLFYGVLQFFL
Outside	206-238	NDKVGELRSRIEVHAARNTATSASQTQATGGAQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9945	-	-

Protein sequence: 239 a.a.

						>T6CP001233 NC_008095:6008478-6009194 [Myxococcus xanthus DK 1622] [TssL]
MDRVTEATKDCFDAAIQLRGSEASAVPPPETLHHRLRGVVDETLRRAAVLGFSHQDAQDMAYALVALIDEVVLGRPEEYR
QLWMPLQLHYFNENVAGDGFFARLNTVRKDPHRHEVLQVYYLCMLFGFQGRYRIRGGELELMTLIDTVQKDLERARPFDF
DVLSPHGDRPTESLLSKRKKASMVSISAGALAVAVLFYGVLQFFLNDKVGELRSRIEVHAARNTATSASQTQATGGAQ*					

Nucleotide sequence: 717 bp

						>T6CP001233 NC_008095:6008478-6009194 [Myxococcus xanthus DK 1622] [TssL]
ATGGACCGAGTCACCGAAGCCACGAAGGATTGTTTCGACGCCGCCATCCAACTGCGCGGCTCCGAAGCCTCGGCGGTGCC
CCCGCCCGAGACGCTGCACCACCGCCTTCGCGGCGTGGTGGACGAGACGCTGCGCCGCGCCGCCGTGCTGGGCTTCAGCC
ATCAGGATGCCCAGGACATGGCCTACGCGCTCGTGGCGCTCATCGACGAGGTCGTCCTGGGCCGCCCGGAGGAGTACCGA
CAGCTCTGGATGCCCCTCCAGCTCCACTACTTCAACGAGAACGTCGCTGGCGACGGCTTCTTCGCGCGCCTCAACACCGT
GCGAAAGGACCCGCACCGGCACGAGGTGCTGCAGGTCTACTATCTGTGCATGCTCTTCGGCTTCCAGGGCCGCTACCGCA
TCCGCGGCGGCGAGCTGGAGCTGATGACGCTCATCGACACGGTGCAGAAGGACCTGGAGCGCGCGCGGCCCTTCGACTTC
GACGTGCTGTCGCCGCACGGGGACCGGCCCACCGAATCGCTGCTCTCCAAGCGCAAGAAGGCGTCAATGGTGAGCATCTC
CGCCGGGGCGCTCGCGGTGGCCGTCCTGTTCTACGGCGTGCTGCAGTTCTTCCTCAACGACAAGGTCGGTGAGCTGAGAA
GCCGCATCGAGGTCCACGCGGCCCGCAACACGGCGACGAGCGCCAGCCAGACGCAGGCAACGGGGGGGGCGCAGTGA					