SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011373
Component protein type	TagL
T6SS ID (Type)	T6SS00127 (Type i1)
Strain	Myxococcus xanthus DK 1622
Replicon	chromosome
Sequence	Protein sequence (616 a.a.); Nucleotide sequence (1848 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Konovalova A, Petters T, Søgaard-Andersen L. Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. PMID: 19895646 [3] Chang YW, Chen S, Tocheva EI, et al. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961. Epub 2014 May 11. PMID: 24813625

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MXAN_RS23325
Coordinate (Strand)	6001993..6003840 (-)
NCBI ID	WP_011554785.1
RefSeq	NC_008095
Uniprot ID	Q1D313_MYXXD, A0A7Y4HE96_MYXXA
KEGG ID	mxa:MXAN_4799
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
OmpA	PF00691.22	9.9e-18	500..596

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-615	MRRKELLHRGLALALALTSLSAGAQEAPVLRGFDLERLELNPGAEGSLLVGMGELLPVGAFRATVMMHYAHQPLVYMDNLIREQPEVAIVSGRTTAHLAAAYAPLDWLQVGLQVPMVAFQGGDDVGMARYGIAVPASQALSTPVVSARFGLLRQTEAGGVDLALDVAAGLPLGSEGAFARDDSGLRLSPRVMVGRHFGFIRAGLEVGYLQRPRVLLTDRTGETEDEVGQELRLGVALATTGKRLRWELNVRGMLPLTDQPTSVELLPGARYLVGSSLEMFALAGVGVGAAPGTPLFRLMVGGAFGNVTPPRGPGESSVRCEMGLPPEAKIQECPDMDEDGDGVRNADDKCPLVPGDVARQGCSAQDTDGDGIEDMLDGCPLEHGPAARQGCPIPDQDGDEVPDEIDSCPSDPGPADNRGCPVRDTDGDGIDNDKDECPNEAGPIERNGCPESDTDKDGVPNRADSCVNEPGDAKNLGCPLHILPLVEIRPDRLVVMGKVFFEPTQARIQKRSYELLDWVARVIREHPEIPLVVVGAHTDDRGFPDDNRRLSQGRAEAVRQYLIGRDIAPERLKALGYGQERPIDSNATSNGRENNRRVEFLLADPQQDKSERGQR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.8801	pos: 24-25. AGA-QE.	MRRKELLHRGLALALALTSLSAGA

Protein sequence: 616 a.a.

						>T6CP011373 NC_008095:c6003840-6001993 [Myxococcus xanthus DK 1622] [TagL]
MRRKELLHRGLALALALTSLSAGAQEAPVLRGFDLERLELNPGAEGSLLVGMGELLPVGAFRATVMMHYAHQPLVYMDNL
IREQPEVAIVSGRTTAHLAAAYAPLDWLQVGLQVPMVAFQGGDDVGMARYGIAVPASQALSTPVVSARFGLLRQTEAGGV
DLALDVAAGLPLGSEGAFARDDSGLRLSPRVMVGRHFGFIRAGLEVGYLQRPRVLLTDRTGETEDEVGQELRLGVALATT
GKRLRWELNVRGMLPLTDQPTSVELLPGARYLVGSSLEMFALAGVGVGAAPGTPLFRLMVGGAFGNVTPPRGPGESSVRC
EMGLPPEAKIQECPDMDEDGDGVRNADDKCPLVPGDVARQGCSAQDTDGDGIEDMLDGCPLEHGPAARQGCPIPDQDGDE
VPDEIDSCPSDPGPADNRGCPVRDTDGDGIDNDKDECPNEAGPIERNGCPESDTDKDGVPNRADSCVNEPGDAKNLGCPL
HILPLVEIRPDRLVVMGKVFFEPTQARIQKRSYELLDWVARVIREHPEIPLVVVGAHTDDRGFPDDNRRLSQGRAEAVRQ
YLIGRDIAPERLKALGYGQERPIDSNATSNGRENNRRVEFLLADPQQDKSERGQR*					

Nucleotide sequence: 1848 bp

						>T6CP011373 NC_008095:c6003840-6001993 [Myxococcus xanthus DK 1622] [TagL]
ATGAGAAGAAAAGAGCTCCTGCACCGCGGGCTCGCGCTGGCACTCGCGCTGACGTCGCTGTCCGCCGGGGCGCAGGAAGC
ACCCGTGTTGCGAGGCTTCGACCTGGAGCGCCTGGAGCTGAATCCCGGCGCGGAGGGTTCGCTCCTCGTCGGCATGGGCG
AACTGCTTCCCGTGGGGGCGTTCCGCGCGACGGTGATGATGCACTACGCACATCAGCCGCTCGTCTACATGGACAACCTC
ATTCGCGAGCAGCCCGAGGTGGCCATCGTCTCTGGACGCACCACGGCGCATCTGGCTGCCGCGTATGCCCCGTTGGACTG
GCTCCAGGTGGGCCTGCAGGTTCCCATGGTGGCGTTCCAGGGCGGCGATGACGTTGGCATGGCGCGTTATGGGATTGCCG
TGCCCGCCAGTCAGGCGCTGTCCACGCCGGTGGTGAGCGCGCGCTTCGGCTTGCTGAGGCAGACGGAAGCGGGTGGGGTG
GACCTGGCCCTGGATGTGGCGGCGGGGCTGCCGCTGGGCAGCGAGGGGGCCTTTGCCCGCGATGATAGCGGGCTCCGCTT
GTCGCCGCGGGTGATGGTGGGACGGCACTTCGGCTTCATCCGCGCGGGCCTGGAGGTTGGCTATCTGCAGCGCCCGCGCG
TGCTGCTGACGGACCGCACGGGCGAAACCGAGGATGAGGTGGGCCAGGAGCTGCGCCTGGGCGTGGCGTTGGCCACGACG
GGCAAGCGGTTGCGCTGGGAGCTCAACGTCCGGGGCATGCTGCCGCTGACGGATCAGCCGACCTCGGTGGAGTTGCTTCC
TGGCGCTCGCTACCTGGTGGGCTCGTCGCTGGAGATGTTCGCGCTGGCGGGCGTGGGCGTGGGCGCGGCGCCCGGCACGC
CGCTGTTCCGGCTGATGGTGGGCGGGGCTTTCGGCAACGTGACGCCGCCGCGGGGCCCGGGTGAGTCCTCGGTCCGGTGT
GAGATGGGGCTGCCGCCCGAGGCGAAGATTCAAGAGTGCCCGGACATGGACGAGGACGGCGACGGCGTGCGTAACGCCGA
CGACAAGTGCCCGCTGGTGCCGGGAGACGTGGCGCGCCAGGGGTGCTCGGCTCAGGACACGGACGGCGACGGAATCGAAG
ACATGTTGGACGGCTGCCCGCTGGAGCACGGTCCCGCCGCGCGCCAGGGCTGCCCGATTCCGGACCAGGACGGTGACGAG
GTCCCCGACGAAATCGACAGTTGCCCGTCGGACCCGGGGCCCGCGGACAACCGGGGCTGCCCGGTGCGGGACACCGACGG
CGACGGCATCGACAACGACAAGGACGAGTGCCCGAACGAGGCCGGTCCCATCGAGCGCAATGGCTGCCCGGAGTCGGACA
CGGACAAGGATGGCGTGCCCAACCGCGCGGACAGCTGTGTGAATGAGCCGGGGGACGCCAAGAACCTGGGCTGCCCGCTG
CACATCCTGCCGCTGGTGGAGATTCGTCCGGACCGCCTGGTGGTGATGGGCAAGGTCTTCTTCGAGCCGACGCAGGCACG
CATCCAGAAGCGCTCCTATGAATTGCTGGATTGGGTGGCGCGCGTCATCCGAGAGCACCCGGAGATTCCGCTCGTCGTGG
TGGGCGCGCACACGGACGACCGGGGCTTCCCGGACGACAACCGCCGCCTGTCGCAGGGGCGTGCGGAGGCCGTGCGCCAG
TACCTCATCGGCCGGGATATCGCGCCCGAGCGGCTGAAGGCCCTGGGCTACGGTCAGGAGCGCCCCATCGACAGCAACGC
GACGTCGAACGGCCGTGAGAACAACCGCCGCGTGGAGTTCCTGCTGGCGGACCCACAGCAAGACAAGTCCGAGCGCGGTC
AGCGCTGA					