Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   SM130_RS16995 Genome accession   NZ_CP139348
Coordinates   3691815..3692000 (+) Length   61 a.a.
NCBI ID   WP_102824888.1    Uniprot ID   A0A2N8S1P9
Organism   Stutzerimonas stutzeri strain KC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3686815..3697000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM130_RS16975 (SM130_16975) coaE 3686890..3687498 (-) 609 WP_102824892.1 dephospho-CoA kinase -
  SM130_RS16980 (SM130_16980) pilD 3687650..3688519 (-) 870 WP_102824891.1 A24 family peptidase Machinery gene
  SM130_RS16985 (SM130_16985) pilC 3688520..3689740 (-) 1221 WP_102824890.1 type II secretion system F family protein Machinery gene
  SM130_RS16990 (SM130_16990) pilB 3689743..3691446 (-) 1704 WP_102824889.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SM130_RS16995 (SM130_16995) pilA 3691815..3692000 (+) 186 WP_102824888.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  SM130_RS17000 (SM130_17000) - 3692015..3692461 (+) 447 WP_146029737.1 hypothetical protein -
  SM130_RS17005 (SM130_17005) - 3692532..3692711 (+) 180 WP_102824887.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SM130_RS17010 (SM130_17010) - 3693109..3693918 (+) 810 WP_219725296.1 hypothetical protein -
  SM130_RS17015 (SM130_17015) - 3693920..3694756 (+) 837 WP_181019250.1 hypothetical protein -
  SM130_RS17020 (SM130_17020) - 3694753..3695649 (+) 897 WP_102824886.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6594.84 Da        Isoelectric Point: 10.2806

>NTDB_id=909044 SM130_RS16995 WP_102824888.1 3691815..3692000(+) (pilA) [Stutzerimonas stutzeri strain KC]
MKSIKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRSKSNDTVANADAKNTLTVMSAAQL

Nucleotide


Download         Length: 186 bp        

>NTDB_id=909044 SM130_RS16995 WP_102824888.1 3691815..3692000(+) (pilA) [Stutzerimonas stutzeri strain KC]
ATGAAGTCCATCAAGAAGCAAAAAGGTTTTACCCTGATTGAGCTGATGATCGTGGTTGCGATCATTGGCATTCTGGCTGC
CATCGCGATTCCGCAGTTCAATGAGTACCGCTCTAAGTCGAACGATACCGTAGCCAACGCGGACGCGAAAAACACCCTTA
CCGTTATGTCTGCCGCACAGCTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2N8S1P9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

60.784

83.607

0.508

  pilA Vibrio parahaemolyticus RIMD 2210633

60

81.967

0.492

  pilA Ralstonia pseudosolanacearum GMI1000

60.417

78.689

0.475

  pilA Pseudomonas aeruginosa PAK

76.316

62.295

0.475

  pilA Acinetobacter baumannii strain A118

85.294

55.738

0.475

  pilA2 Legionella pneumophila str. Paris

63.043

75.41

0.475

  pilA2 Legionella pneumophila strain ERS1305867

63.043

75.41

0.475

  pilA Vibrio cholerae strain A1552

77.778

59.016

0.459

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.778

59.016

0.459

  pilA Vibrio cholerae C6706

77.778

59.016

0.459

  pilA Acinetobacter nosocomialis M2

82.353

55.738

0.459

  pilA/pilAI Pseudomonas stutzeri DSM 10701

77.143

57.377

0.443

  pilA Vibrio campbellii strain DS40M4

75

59.016

0.443

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.057

86.885

0.426

  pilA/pilAII Pseudomonas stutzeri DSM 10701

63.415

67.213

0.426

  pilA/pilA1 Eikenella corrodens VA1

66.667

63.934

0.426

  pilA/pilA4 Thermus thermophilus HB27

56.818

72.131

0.41

  comF Acinetobacter baylyi ADP1

63.158

62.295

0.393

  pilA/pilA1 Synechocystis sp. PCC 6803

42.857

91.803

0.393

  pilE Acinetobacter baumannii D1279779

53.333

73.77

0.393

  pilE Legionella pneumophila strain ERS1305867

46.939

80.328

0.377

  pilE Legionella pneumophila str. Paris

46.939

80.328

0.377

  pilA Haemophilus influenzae Rd KW20

67.647

55.738

0.377

  pilA Haemophilus influenzae 86-028NP

67.647

55.738

0.377

  comE Acinetobacter baylyi ADP1

62.162

60.656

0.377

  fimU Acinetobacter baumannii D1279779

36.066

100

0.361

  pilA Glaesserella parasuis strain SC1401

66.667

54.098

0.361

  pilX Neisseria meningitidis 8013

64.706

55.738

0.361

  pilE Neisseria gonorrhoeae MS11

64.706

55.738

0.361

  pilE Neisseria gonorrhoeae strain FA1090

64.706

55.738

0.361

  pilL Neisseria gonorrhoeae MS11

62.857

57.377

0.361