Detailed information    

experimental Experimentally validated

Overview

Name   pilE   Type   Machinery gene
Locus tag   LPP_RS03425 Genome accession   NC_006368
Coordinates   732230..732679 (-) Length   149 a.a.
NCBI ID   WP_010946364.1    Uniprot ID   Q5ZXV4
Organism   Legionella pneumophila str. Paris     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilE is the DNA receptor at the tip of a pilus composed of PilA2 subunits.


Genomic Context

Location: 727230..737679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS03405 (lpp0677) - 727505..727897 (-) 393 WP_010946360.1 hypothetical protein -
  LPP_RS03410 (lpp0678) - 728034..728411 (+) 378 WP_010946361.1 hypothetical protein -
  LPP_RS03415 (lpp0679) - 728612..729919 (+) 1308 WP_011213222.1 tetratricopeptide repeat protein -
  LPP_RS03420 (lpp0680) comEC 729945..732152 (-) 2208 WP_010946363.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LPP_RS03425 (lpp0681) pilE 732230..732679 (-) 450 WP_010946364.1 type IV pilin protein Machinery gene
  LPP_RS03430 (lpp0682) - 732691..736200 (-) 3510 WP_011213223.1 pilus assembly protein -
  LPP_RS03435 (lpp0683) - 736213..736725 (-) 513 WP_010946366.1 pilus assembly protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16296.57 Da        Isoelectric Point: 7.6922

>NTDB_id=1273 LPP_RS03425 WP_010946364.1 732230..732679(-) (pilE) [Legionella pneumophila str. Paris]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1273 LPP_RS03425 WP_010946364.1 732230..732679(-) (pilE) [Legionella pneumophila str. Paris]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGCATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAATCCCGTCGTGCCGATGCTC
ACGCCACATTGACACAAGATCAAATTATTTTAGAACGCTGTTATTCACAGAATTTTTCTTATGCTGCGGCGTGTGGCGCC
TTACCAGCATTTCCTCAAACAACGCCGAACGGGTACTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACTGCAACCCCTGTTGGAACTCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATTAACCAGGCCAATGTAAAAACAG
CAGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-38)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(39-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]