Detailed information    

experimental Experimentally validated

Overview

Name   pilE   Type   Machinery gene
Locus tag   GCO85_RS03355 Genome accession   NZ_CP048618
Coordinates   700030..700479 (-) Length   149 a.a.
NCBI ID   WP_010946364.1    Uniprot ID   Q5ZXV4
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilE is the DNA receptor at the tip of a pilus composed of PilA2 subunits.


Genomic Context

Location: 695030..705479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS03335 (GCO85_03345) - 695275..695667 (-) 393 WP_027266704.1 hypothetical protein -
  GCO85_RS03340 (GCO85_03350) - 695804..696181 (+) 378 WP_011945787.1 hypothetical protein -
  GCO85_RS03345 (GCO85_03355) - 696382..697689 (+) 1308 WP_042646571.1 tetratricopeptide repeat protein -
  GCO85_RS03350 (GCO85_03360) comEC 697745..699952 (-) 2208 WP_061470007.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GCO85_RS03355 (GCO85_03365) pilE 700030..700479 (-) 450 WP_010946364.1 type IV pilin protein Machinery gene
  GCO85_RS03360 (GCO85_03370) - 700491..704000 (-) 3510 WP_014841115.1 PilC/PilY family type IV pilus protein -
  GCO85_RS03365 (GCO85_03375) - 704013..704525 (-) 513 WP_014841116.1 pilus assembly protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16296.57 Da        Isoelectric Point: 7.6922

>NTDB_id=1260 GCO85_RS03355 WP_010946364.1 700030..700479(-) (pilE) [Legionella pneumophila strain ERS1305867]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1260 GCO85_RS03355 WP_010946364.1 700030..700479(-) (pilE) [Legionella pneumophila strain ERS1305867]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATACAAAAATCCCGTCGTGCTGATGCTC
ATGCCACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCGGCAGCGTGTGGCGCC
CTACCAGCATTTCCTCAAACAACGCCGAATGGGTATTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATTAATCAAGCCAATGTAAAAACAG
CCGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-38)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(39-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila str. Paris

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]