Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   GCO85_RS03350 Genome accession   NZ_CP048618
Coordinates   697745..699952 (-) Length   735 a.a.
NCBI ID   WP_061470007.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 692745..704952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS03330 (GCO85_03340) - 693221..695149 (-) 1929 WP_011945785.1 hypothetical protein -
  GCO85_RS03335 (GCO85_03345) - 695275..695667 (-) 393 WP_027266704.1 hypothetical protein -
  GCO85_RS03340 (GCO85_03350) - 695804..696181 (+) 378 WP_011945787.1 hypothetical protein -
  GCO85_RS03345 (GCO85_03355) - 696382..697689 (+) 1308 WP_042646571.1 tetratricopeptide repeat protein -
  GCO85_RS03350 (GCO85_03360) comEC 697745..699952 (-) 2208 WP_061470007.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GCO85_RS03355 (GCO85_03365) pilE 700030..700479 (-) 450 WP_010946364.1 type IV pilin protein Machinery gene
  GCO85_RS03360 (GCO85_03370) - 700491..704000 (-) 3510 WP_014841115.1 PilC/PilY family type IV pilus protein -
  GCO85_RS03365 (GCO85_03375) - 704013..704525 (-) 513 WP_014841116.1 pilus assembly protein -

Sequence

Protein


Download         Length: 735 a.a.        Molecular weight: 83266.86 Da        Isoelectric Point: 9.7684

>NTDB_id=1246 GCO85_RS03350 WP_061470007.1 697745..699952(-) (comEC) [Legionella pneumophila strain ERS1305867]
MEIFCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGCGFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQ
RPNKTQFHFAIEKINNNPAQGLIQLNWYNKPPVLRAGQRWQLSVKLKKPRNFRNPGGFNYVRYLAARHIHWTGYIRYGNN
KLISNQPPSFSWLTLREKIGDALTKLAPNTETAGIIEALTLNVTHHISSDHWNLFRRTGTVHLFGISGEHIALVSGIFFI
VIRWIWSMSYRCCLFLPAHTIASIGGLLAALPYAFLAGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIE
PHAVFLQGFYFSFLAVACLLLTHQRWPLKNYKKTLTLQLSCLIGLMPLTLHWYSYGSINGFLANLFSIPLVSFLIVPLAL
MTMITSPLHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMMLAMTAGLFLRVLLPVKPFKHLAILWIILPL
FPPRTAIYPGEALINILDVGQGLAVAIQTQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGG
LNSLEKEMQINQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIKESFTKKNNNSCILQVSNSAGRVLLTGDIEKAAEE
YLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVNPLYAIASLGFDNRFKFPHSKTLQSMKTLDITFFRTDECGMAAIKL
PASGQVHRPSCMQAN

Nucleotide


Download         Length: 2208 bp        

>NTDB_id=1246 GCO85_RS03350 WP_061470007.1 697745..699952(-) (comEC) [Legionella pneumophila strain ERS1305867]
ATGGAAATTTTTTGCTTTTTAATAGGCGTACTTTATGCCTATACCTATAATATTTATCTAACTATTGCGATTCCTTTACT
CTTTTATATCACACCAAGATATCCAATTGTCCTGTTCTTCATCGCCGGGTGCGGCTTTGCCCTGATTCATCAGTGGATTG
TATCTCCCAAGGGCATGCCTGATACGCAAGTAATCACTCAAGCCCAATTGGAAGGAACAATTGCTTCCATCCCCATGCAA
CGTCCTAACAAAACTCAATTCCATTTTGCAATTGAAAAAATTAATAATAACCCTGCTCAAGGCTTGATCCAATTAAACTG
GTATAACAAACCTCCTGTCTTACGAGCCGGTCAGCGATGGCAACTTTCAGTGAAACTAAAAAAACCTCGTAATTTCCGCA
ACCCAGGAGGTTTTAATTATGTACGTTACCTGGCGGCTCGTCATATCCATTGGACTGGTTATATTCGTTATGGGAATAAT
AAGCTAATTAGCAATCAACCCCCTTCTTTCAGTTGGTTAACACTGCGTGAAAAAATAGGGGATGCTTTGACAAAACTGGC
ACCGAATACAGAAACGGCAGGCATCATCGAAGCATTGACGTTAAATGTTACTCACCATATCAGTTCAGATCATTGGAATT
TGTTCCGAAGAACAGGAACGGTACATTTATTCGGGATTTCAGGGGAGCACATCGCTCTGGTCTCCGGCATTTTCTTTATA
GTGATAAGATGGATTTGGTCAATGAGCTATCGTTGCTGTTTGTTCTTACCGGCCCATACCATAGCGAGTATCGGTGGATT
ACTGGCCGCCTTGCCCTATGCTTTTTTAGCCGGGTTTGAACCTCCTGTACAGCGTGCTTTAATTGGTTGTGTACTTTACA
CAATATACGCCATAGGAAAACAACGTTTTACACCATGGCAAATATGGAGATACGCCTTATTTTTAGTCTTGTGTATTGAA
CCCCATGCCGTATTCCTGCAAGGTTTTTATTTTTCATTTCTTGCTGTGGCCTGTCTTCTATTGACGCACCAACGTTGGCC
ACTGAAAAATTATAAAAAAACACTGACATTACAATTAAGCTGCCTCATTGGATTGATGCCCTTGACTCTCCATTGGTACT
CATATGGTTCTATTAATGGATTCCTTGCCAATTTATTCTCCATACCTTTAGTCAGCTTTCTGATAGTACCCTTGGCTTTA
ATGACTATGATAACGAGTCCGCTTCATTGGTCCTGGATATTGATGAAACCATTGGCATGGCTTGTTGCCCTGTTACTGCA
AGGACTAACGTATGTTGAGCAAATAGCAGGGATTAATATCACCTGGTCCATCACCTCGGTAACAATGATGCTCGCTATGA
CAGCAGGTCTGTTTTTAAGGGTATTATTACCTGTCAAGCCATTCAAACACTTGGCTATCCTTTGGATTATCCTTCCCTTA
TTTCCGCCTCGTACTGCAATTTATCCAGGGGAAGCATTAATTAATATTTTGGATGTTGGGCAAGGACTGGCTGTTGCGAT
ACAAACGCAACATCATGTTTTACTATACGATACGGGAGATTCCTATTTTCAGGGGAGTGATTTAGGGCAAATGGCGATAA
TACCCTTTTTCAGGTCAATTGGAATAAAAACAATTGACAAAATAGTAATAAGCCATCCTGATAAAGATCATAAGGGCGGA
TTAAATTCCCTTGAAAAAGAGATGCAAATCAATCAATTAATAGTGAATGATCCCTATTTTTATAAACGTGGAAGTAATTG
TCATTCATACCCACAATGGCAATGGGATGGGGTGAGTTTTCGTTTTTTACCCATCAAGGAATCTTTCACTAAAAAAAATA
ACAATTCTTGTATCTTACAGGTAAGTAATTCAGCTGGCAGGGTTTTATTAACGGGAGATATTGAAAAAGCGGCTGAAGAG
TACCTGGTAAGAAACTACGGCAGTCAGCTGGCATCCGAGATTTTAGTTGTGCCTCATCATGGGAGTAAAACGTCTTCATC
CTATCGATTTTTGCTGGAAGTAAACCCGCTTTATGCCATTGCATCCTTAGGATTTGATAATCGTTTCAAATTTCCACATT
CTAAAACTTTGCAAAGCATGAAAACATTAGATATCACTTTTTTTAGAACAGACGAATGCGGAATGGCAGCAATCAAACTA
CCTGCATCAGGTCAAGTTCACAGACCAAGTTGTATGCAGGCTAATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(21-158)

Metallo-beta-lactamase superfamily (Lactamase_B)

(498-577)

Competence protein (Competence)

(199-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Legionella pneumophila strain Lp02

96.866

99.864

0.967

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]