Detailed information    

experimental Experimentally validated

Overview

Name   fimU   Type   Machinery gene
Locus tag   ABD1_RS15750 Genome accession   NC_020547
Coordinates   3326017..3326490 (-) Length   157 a.a.
NCBI ID   WP_001214065.1    Uniprot ID   N9K1Q3
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3321017..3331490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15735 (ABD1_30530) pilX 3323646..3324464 (-) 819 WP_000149372.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ABD1_RS15740 (ABD1_30540) pilW 3324461..3325462 (-) 1002 WP_000079197.1 PilW family protein Machinery gene
  ABD1_RS15745 (ABD1_30550) pilV 3325463..3326023 (-) 561 WP_002194578.1 type IV pilus modification protein PilV Machinery gene
  ABD1_RS15750 (ABD1_30560) fimU 3326017..3326490 (-) 474 WP_001214065.1 pilus assembly FimT family protein Machinery gene
  ABD1_RS15755 (ABD1_30570) ispH 3326660..3327610 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABD1_RS15760 (ABD1_30580) gmk 3327733..3328362 (+) 630 WP_000015937.1 guanylate kinase -
  ABD1_RS15765 (ABD1_30590) rpoZ 3328435..3328713 (+) 279 WP_000135049.1 DNA-directed RNA polymerase subunit omega -
  ABD1_RS18215 - 3328752..3328882 (+) 131 Protein_3129 hypothetical protein -
  ABD1_RS15770 (ABD1_30600) - 3328922..3331027 (+) 2106 WP_001117592.1 RelA/SpoT family protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16837.43 Da        Isoelectric Point: 8.5355

>NTDB_id=1068 ABD1_RS15750 WP_001214065.1 3326017..3326490(-) (fimU) [Acinetobacter baumannii D1279779]
MRGIIPQEGFTLVELMVTIAVMAIIALMAAPSMSNLLESKRLDANQRDLINTLSEAKSQAILGRQNVSVNLNSTASNTPT
SLNWQTASNNTLELKNIAADGTQSSLTTTTLAFNANGVVANITQDTLLSICNSRINKKKVIILTKLGTLVFKAEETC

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1068 ABD1_RS15750 WP_001214065.1 3326017..3326490(-) (fimU) [Acinetobacter baumannii D1279779]
ATGAGGGGAATTATTCCACAAGAAGGGTTCACCTTGGTCGAGCTAATGGTGACGATTGCGGTCATGGCTATTATTGCATT
GATGGCTGCGCCATCAATGTCAAATTTATTAGAAAGTAAACGTTTAGATGCTAATCAAAGAGACTTAATCAACACTTTAT
CAGAAGCGAAAAGTCAGGCAATTTTAGGCCGTCAGAATGTTTCTGTTAACTTGAACTCAACAGCTTCAAATACACCCACT
TCATTAAATTGGCAGACAGCTTCTAATAATACTCTTGAGCTAAAAAATATAGCCGCTGATGGTACGCAAAGTTCTTTAAC
GACTACCACTTTAGCTTTTAATGCAAATGGAGTAGTGGCAAATATAACTCAAGATACGCTTTTATCTATTTGTAATTCTA
GAATAAATAAAAAGAAAGTCATTATTTTGACCAAGCTTGGCACTTTAGTTTTTAAGGCCGAGGAGACTTGTTAA

Domains

Predicted by InterproScan.

Type II transport protein GspH (GspH)

(47-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9K1Q3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]