Detailed information    

experimental Experimentally validated

Overview

Name   pilX   Type   Machinery gene
Locus tag   ABD1_RS15735 Genome accession   NC_020547
Coordinates   3323646..3324464 (-) Length   272 a.a.
NCBI ID   WP_000149372.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3318646..3329464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15720 (ABD1_30500) pilE 3318913..3319338 (-) 426 WP_000788345.1 type IV pilin protein Machinery gene
  ABD1_RS15725 (ABD1_30510) pilY2 3319335..3319817 (-) 483 WP_001046490.1 type IV pilin protein Machinery gene
  ABD1_RS15730 (ABD1_30520) pilY1 3319828..3323634 (-) 3807 WP_000768961.1 PilC/PilY family type IV pilus protein Machinery gene
  ABD1_RS15735 (ABD1_30530) pilX 3323646..3324464 (-) 819 WP_000149372.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ABD1_RS15740 (ABD1_30540) pilW 3324461..3325462 (-) 1002 WP_000079197.1 PilW family protein Machinery gene
  ABD1_RS15745 (ABD1_30550) pilV 3325463..3326023 (-) 561 WP_002194578.1 type IV pilus modification protein PilV Machinery gene
  ABD1_RS15750 (ABD1_30560) fimU 3326017..3326490 (-) 474 WP_001214065.1 pilus assembly FimT family protein Machinery gene
  ABD1_RS15755 (ABD1_30570) ispH 3326660..3327610 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABD1_RS15760 (ABD1_30580) gmk 3327733..3328362 (+) 630 WP_000015937.1 guanylate kinase -
  ABD1_RS15765 (ABD1_30590) rpoZ 3328435..3328713 (+) 279 WP_000135049.1 DNA-directed RNA polymerase subunit omega -
  ABD1_RS18215 - 3328752..3328882 (+) 131 Protein_3129 hypothetical protein -

Sequence

Protein


Download         Length: 272 a.a.        Molecular weight: 28905.76 Da        Isoelectric Point: 5.6905

>NTDB_id=1065 ABD1_RS15735 WP_000149372.1 3323646..3324464(-) (pilX) [Acinetobacter baumannii D1279779]
MTHLNYKNIKGRSKETGATLIVVLIILLIVISVGVLAIRVAIVSLKVATNSQVGQLNFQSSDTPLELIVQMNPTTLTNIT
NVIGAALKAHESNPGAEYNFCYKPTSTSVSFAQTRGASLLRAGSANNAVVEDGGVAGFCDLTSDYGSNRQAVVTQVAVSI
PTDAMADIPGSNLPRDINLSEGTQLPKSMLSTQRIRVITTAFLPAYASTSLENLQFDCLSTSSAKISDNFDSALTDKQTL
ADCLANHNVPFSTQVQEFNYTNKLTEITAPGS

Nucleotide


Download         Length: 819 bp        

>NTDB_id=1065 ABD1_RS15735 WP_000149372.1 3323646..3324464(-) (pilX) [Acinetobacter baumannii D1279779]
ATGACCCATTTAAATTATAAAAATATTAAAGGTCGATCTAAGGAAACGGGTGCCACACTTATTGTTGTACTTATTATTTT
ATTGATTGTTATTTCTGTCGGGGTTTTGGCAATAAGAGTAGCGATTGTTTCTCTAAAAGTTGCAACTAATAGCCAAGTTG
GCCAATTAAACTTTCAATCTTCAGATACTCCGTTAGAGCTTATTGTACAGATGAACCCAACCACATTGACAAATATTACC
AATGTGATTGGAGCAGCACTGAAAGCACATGAAAGTAACCCTGGTGCGGAATATAACTTTTGCTATAAACCAACTTCTAC
ATCTGTAAGTTTTGCTCAAACTCGGGGGGCAAGTTTGCTTCGAGCTGGCAGCGCTAATAATGCTGTGGTTGAAGATGGTG
GAGTAGCAGGTTTCTGCGATTTAACAAGTGACTATGGGAGTAATCGTCAAGCGGTAGTTACTCAAGTTGCTGTCAGTATC
CCAACCGATGCCATGGCTGATATTCCGGGTTCAAATCTACCTCGGGACATAAATCTTTCCGAAGGAACCCAACTTCCTAA
AAGCATGCTATCTACGCAACGTATCCGGGTTATTACAACAGCGTTTTTACCTGCTTATGCCTCAACTTCTTTAGAAAATC
TGCAATTTGATTGCTTAAGTACGAGTTCCGCAAAAATTAGTGACAACTTTGATAGTGCATTAACGGATAAGCAAACTCTA
GCTGATTGTTTGGCAAACCATAACGTACCTTTTAGTACTCAAGTTCAAGAATTTAACTATACCAATAAGCTGACTGAAAT
CACGGCACCAGGTTCATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]