Detailed information NTG0001064

Experimentally validated

Overview

• Name: pilY2
• Type: Machinery gene
• Locus tag: ABD1_RS15725
• Genome accession: NC_020547
• Coordinates: 3319335..3319817 (-)
• Length: 160 a.a.
• NCBI ID: WP_001046490.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: assembly of type IV pilus DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 3314335..3324817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS15690 (ABD1_30440)	-	3314445..3315476 (+)	1032	WP_015451632.1	lipase secretion chaperone	-
ABD1_RS15695 (ABD1_30450)	-	3315591..3316532 (+)	942	WP_104133477.1	lipase family alpha/beta hydrolase	-
ABD1_RS15700 (ABD1_30460)	rplS	3316585..3316953 (-)	369	WP_000014562.1	50S ribosomal protein L19	-
ABD1_RS15705 (ABD1_30470)	trmD	3317161..3317901 (-)	741	WP_000464598.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
ABD1_RS15710 (ABD1_30480)	rimM	3317947..3318495 (-)	549	WP_000189236.1	ribosome maturation factor RimM	-
ABD1_RS15715 (ABD1_30490)	rpsP	3318515..3318766 (-)	252	WP_000260334.1	30S ribosomal protein S16	-
ABD1_RS15720 (ABD1_30500)	pilE	3318913..3319338 (-)	426	WP_000788345.1	type IV pilin protein	Machinery gene
ABD1_RS15725 (ABD1_30510)	pilY2	3319335..3319817 (-)	483	WP_001046490.1	type IV pilin protein	Machinery gene
ABD1_RS15730 (ABD1_30520)	pilY1	3319828..3323634 (-)	3807	WP_000768961.1	PilC/PilY family type IV pilus protein	Machinery gene
ABD1_RS15735 (ABD1_30530)	pilX	3323646..3324464 (-)	819	WP_000149372.1	PilX N-terminal domain-containing pilus assembly protein	Machinery gene

Sequence

Protein

• Length: 160 a.a.
• Molecular weight: 17670.94 Da
• Isoelectric Point: 8.4737

>NTDB_id=1064 ABD1_RS15725 WP_001046490.1 3319335..3319817(-) (pilY2) [Acinetobacter baumannii D1279779]
MNKYYILRFNQGFTLIELMVVIVIVAIFASIAIPSYQSYSRRATASAAKSEILKLAEQLEQHKSRNFTYRGFTTTSITLP
RGGYTIQITDDTTTGNLLTNTAANGQTWVIKATTTDSRNFNFIAKNSGLRCQSLTATAVDNDCGGTVTCNVCETNSENWQ

Nucleotide

• Length: 483 bp

>NTDB_id=1064 ABD1_RS15725 WP_001046490.1 3319335..3319817(-) (pilY2) [Acinetobacter baumannii D1279779]
ATGAATAAGTACTACATTTTACGGTTCAATCAGGGTTTTACCTTGATTGAGCTTATGGTCGTGATTGTTATCGTTGCTAT
TTTTGCTTCAATTGCTATCCCTTCATATCAAAGTTATAGCCGTAGAGCTACTGCATCTGCGGCTAAAAGTGAAATTTTGA
AATTAGCTGAACAATTAGAACAGCATAAATCAAGAAATTTTACTTATAGGGGTTTTACCACAACCTCTATAACCCTACCT
CGCGGGGGATATACGATTCAAATTACTGATGATACGACCACAGGAAATTTATTGACGAATACTGCTGCAAATGGGCAAAC
ATGGGTCATTAAAGCGACAACTACAGATTCAAGAAACTTTAATTTTATTGCTAAGAATTCAGGTTTGCGTTGTCAAAGTT
TAACAGCTACAGCTGTTGATAATGACTGTGGTGGAACAGTCACTTGCAATGTATGTGAAACAAATAGTGAGAATTGGCAA
TGA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (34-107)

• Prokaryotic N-terminal methylation motif (N_methyl) (10-32)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Acinetobacter baylyi ADP1	41.279	100	0.444

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]