Detailed information    

experimental Experimentally validated

Overview

Name   pilY1   Type   Machinery gene
Locus tag   ABD1_RS15730 Genome accession   NC_020547
Coordinates   3319828..3323634 (-) Length   1268 a.a.
NCBI ID   WP_000768961.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3314828..3328634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15695 (ABD1_30450) - 3315591..3316532 (+) 942 WP_104133477.1 lipase family alpha/beta hydrolase -
  ABD1_RS15700 (ABD1_30460) rplS 3316585..3316953 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  ABD1_RS15705 (ABD1_30470) trmD 3317161..3317901 (-) 741 WP_000464598.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ABD1_RS15710 (ABD1_30480) rimM 3317947..3318495 (-) 549 WP_000189236.1 ribosome maturation factor RimM -
  ABD1_RS15715 (ABD1_30490) rpsP 3318515..3318766 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  ABD1_RS15720 (ABD1_30500) pilE 3318913..3319338 (-) 426 WP_000788345.1 type IV pilin protein Machinery gene
  ABD1_RS15725 (ABD1_30510) pilY2 3319335..3319817 (-) 483 WP_001046490.1 type IV pilin protein Machinery gene
  ABD1_RS15730 (ABD1_30520) pilY1 3319828..3323634 (-) 3807 WP_000768961.1 PilC/PilY family type IV pilus protein Machinery gene
  ABD1_RS15735 (ABD1_30530) pilX 3323646..3324464 (-) 819 WP_000149372.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ABD1_RS15740 (ABD1_30540) pilW 3324461..3325462 (-) 1002 WP_000079197.1 PilW family protein Machinery gene
  ABD1_RS15745 (ABD1_30550) pilV 3325463..3326023 (-) 561 WP_002194578.1 type IV pilus modification protein PilV Machinery gene
  ABD1_RS15750 (ABD1_30560) fimU 3326017..3326490 (-) 474 WP_001214065.1 pilus assembly FimT family protein Machinery gene
  ABD1_RS15755 (ABD1_30570) ispH 3326660..3327610 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABD1_RS15760 (ABD1_30580) gmk 3327733..3328362 (+) 630 WP_000015937.1 guanylate kinase -

Sequence

Protein


Download         Length: 1268 a.a.        Molecular weight: 135878.74 Da        Isoelectric Point: 7.4603

>NTDB_id=1063 ABD1_RS15730 WP_000768961.1 3319828..3323634(-) (pilY1) [Acinetobacter baumannii D1279779]
MKLKLKNFKPNNLWYAVCSSSMIFTWLMTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTSNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSADGDSRSGQILVAAAPLGDASTLNTMGSQRYKLKQAVASLTASGYTPSAHAYAEAAAYLM
GTTTLKNINLVDAPIYFSYVTYDRRGRVITNYHACTEWNTEGTICNSWDSSSLSNPPVISGLQQASCTANIGWQLLSGTC
YKKTGLITINNLDSGFNKSISGSKNTDQSQYNSPLPAVANRQSCDGQGIYFLSDGEPNKTTDTRSASIMSTALGSTFGAD
FNCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLSSSDVKNACRLSSRTQFDRGGDDLCSPN
QTTNAITAPGYGNGGFFSTQSAQGVTDSVIAFINNLDKVPLEPLTTGAISVPYDALNPKNLQEYGYLRAFEPNPANTYLT
WRGNLKKYHVVLSGTNAGAFEANTGGLVYNATGAFRTGTKDYWNSSTYNDGGKVFFGGSYAKVPLPIAGQNETRDAEGNI
TKYYYAVQSKIRNLFTDVSAVAADGSLTKISTSGTNLLKIPAAPPEGTNPFDTVANTASYVLGKFDPSTGQNILKAFPIS
LKLKILNYLGYSTDISATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDDNGNLTSAREQSILYGTMEGGLHIVDAS
SGIEQMVFVPADILNDSVASKALVVGQSDASAPAHGMDGAWVSDPAYNITTVGSGSSAVSKVTAKQMNIYGGMRMGGSSY
YGLDVLSPTSPKLLFRIGADQTDYSRMGQSWSKPVLANIRYNGSIRRVLIVGGGYDQCYEKPNITLTDACFTNGKAKGNA
VYIIDAKTGQRLWWTSDTGSNTDNANMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRVDLNNNQTKTNSTYSSFGVR
VVRLANLATNDSTYDGTNDYTGGNAPRFYEPPTVTIHDYGIHTFITVGIASGDRSTPLDVYPLTGREGMTPTSALSGRPV
NNVYGIIDRDFVKKNLMSLTDNQLETKDITRTGLRKNPQILRTGETRVAQIFFPTTGVGKGGWYRSLSSTSDGTEKANNS
FRIKGGLKAFEEPMAITGNLIIPVYDPQGTGIVAADPCLPRVVGETDRQTYCLPFGACLNSDGSIDQNKENHSGFETQTG
TNCPVGASECNKNVIGSGIRSVTFVPTEDNPPMTNSCGKLKLSGNEQGTGQWQCTSHLVPTRWYERYR

Nucleotide


Download         Length: 3807 bp        

>NTDB_id=1063 ABD1_RS15730 WP_000768961.1 3319828..3323634(-) (pilY1) [Acinetobacter baumannii D1279779]
ATGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTATGGTATGCCGTCTGTTCAAGCTCAATGATATTCACATGGCT
GATGACAAGCTCTGTGGTGCAAGCAAGTGATTTACAAATTTATGCCTCACCTACAGCAGGTAAAAAAACAATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAGCAATAGTTATGGTGAAAACCGTTTGGCTATGCTTAAAAATGGTATGAATGCT
TTTTTAGCAAGTAACAACCCAGTTTTAAATGATACTCGTGTAGGATTGGGAAATTTCTCTGCAGATGGTGATAGTCGAAG
TGGGCAAATTTTGGTGGCTGCTGCTCCTTTAGGAGATGCAAGTACGTTAAATACAATGGGTTCTCAGCGTTATAAATTAA
AACAAGCAGTTGCTAGCTTAACTGCAAGTGGATATACCCCTTCAGCACATGCTTATGCAGAAGCCGCTGCTTATTTGATG
GGAACAACGACTTTAAAAAATATTAATTTGGTGGATGCACCTATATATTTTTCATATGTAACGTATGATCGACGAGGTCG
AGTAATAACGAATTATCATGCTTGTACAGAATGGAATACAGAAGGAACAATATGTAATAGTTGGGATAGTTCATCTTTAA
GTAATCCCCCCGTAATAAGTGGATTGCAACAAGCCTCATGTACTGCAAATATTGGGTGGCAATTGTTATCAGGTACCTGT
TATAAAAAAACAGGTTTAATAACAATTAATAATTTAGATAGCGGATTCAATAAATCTATTAGTGGATCTAAAAATACAGA
TCAAAGTCAATATAACTCTCCTTTGCCAGCGGTAGCTAATCGTCAAAGCTGTGATGGGCAGGGTATTTATTTTTTGTCAG
ATGGTGAGCCGAATAAAACCACAGATACACGTTCAGCAAGTATCATGTCGACTGCTTTAGGTAGTACTTTTGGAGCTGAT
TTTAATTGTTCTGGAGGTTTGTCTAACACGACAGCAGACTCTGGTTGGGCCTGTATGGGGGAGTTTGCAAAAAGATTATT
TGATAAAACAAAGAATCCTGCTGGAGTTTCTATTCAAACCGCATTTGTTGGTTTTGGTAGTGATTTTTCTAGTTTAAGTT
CTTCTGATGTAAAAAATGCCTGTCGTCTGAGTTCGAGAACCCAATTTGACCGTGGGGGGGATGATCTATGTTCGCCAAAT
CAAACTACAAATGCGATTACAGCACCTGGCTATGGGAATGGTGGCTTTTTTTCAACTCAAAGTGCGCAAGGTGTAACAGA
TAGTGTTATCGCATTTATTAATAACTTAGATAAAGTTCCTTTAGAACCCTTAACCACAGGTGCTATCTCTGTTCCTTATG
ATGCACTAAATCCTAAAAATTTACAGGAATATGGTTATTTACGAGCTTTTGAGCCAAATCCAGCTAACACGTACTTAACA
TGGCGTGGAAATTTAAAGAAATATCATGTTGTTTTATCGGGAACAAATGCTGGTGCTTTTGAAGCTAATACTGGTGGGTT
GGTTTACAACGCAACTGGGGCATTTAGAACGGGTACGAAAGATTATTGGAATAGCTCTACTTATAATGATGGAGGTAAAG
TCTTTTTTGGTGGTTCATATGCAAAAGTTCCTTTACCAATTGCTGGACAAAATGAAACACGTGATGCAGAAGGAAATATT
ACAAAATATTATTACGCAGTACAAAGTAAAATCCGCAACTTATTTACTGATGTTTCCGCCGTTGCAGCAGATGGTAGCTT
AACGAAAATTTCAACTTCTGGAACTAATTTGCTAAAAATTCCAGCTGCTCCACCAGAAGGAACTAATCCTTTTGATACGG
TGGCTAATACAGCAAGTTATGTTTTAGGTAAATTTGATCCATCAACTGGACAAAATATTTTAAAAGCTTTTCCTATAAGC
TTGAAATTAAAAATATTAAATTATTTAGGTTATTCAACAGATATTAGTGCAACAACTCTGCCTTCATCTTTGGTTACATC
GAATGAACCTTATTTATCGATGGGGGGAAGTATTCACTCTTTACCGGTTCAACTAACCTACAATGGAACACTAGATGATA
ATGGGAATTTAACATCTGCCCGAGAACAATCCATCCTCTATGGAACCATGGAAGGCGGGTTACATATTGTGGATGCTTCA
TCTGGTATTGAGCAAATGGTCTTTGTTCCTGCAGATATTTTAAATGATTCAGTTGCTTCCAAAGCTTTAGTTGTTGGTCA
AAGTGATGCTTCAGCTCCCGCTCATGGTATGGACGGAGCTTGGGTATCTGATCCAGCCTATAATATTACTACAGTGGGAA
GTGGTAGCTCGGCAGTATCAAAAGTAACTGCAAAGCAAATGAATATTTATGGCGGCATGCGTATGGGAGGAAGTAGCTAC
TACGGACTAGATGTATTAAGCCCTACTTCACCGAAACTGCTTTTTAGAATAGGGGCAGACCAGACTGACTATAGCCGTAT
GGGTCAAAGCTGGTCTAAACCCGTACTCGCGAACATCCGTTATAACGGTTCTATTAGACGTGTCCTGATTGTTGGCGGTG
GTTATGATCAGTGTTATGAAAAACCAAATATTACGTTGACTGACGCTTGCTTTACCAATGGAAAAGCAAAAGGAAATGCT
GTCTATATTATTGACGCAAAAACTGGTCAGCGTTTATGGTGGACAAGTGATACAGGCTCTAATACTGATAACGCCAATAT
GAAGCATAGTATTGTTAGCCGTATTAGTACTTTAGACCGTGATGCTGATGGCTTAGTCGACCATCTATATTTTGGAGATT
TAGGCGGACAAATTTTTCGCGTGGACCTTAATAATAATCAGACAAAAACCAATTCGACCTATAGCAGTTTTGGTGTCAGA
GTTGTGCGTTTAGCGAATTTAGCAACAAATGATTCAACTTATGATGGCACAAATGATTATACAGGTGGGAATGCTCCTCG
TTTTTATGAGCCTCCAACAGTAACGATCCACGATTATGGAATTCACACTTTTATTACAGTAGGCATTGCTTCAGGGGACC
GTAGTACACCTTTAGATGTTTACCCACTCACAGGTCGTGAAGGTATGACGCCTACAAGTGCATTAAGTGGACGTCCTGTA
AATAATGTATATGGAATTATTGATAGAGACTTTGTTAAAAAGAACTTAATGTCTTTAACTGATAATCAGCTTGAAACAAA
AGATATTACACGGACAGGCTTAAGAAAAAATCCACAAATTCTAAGAACAGGTGAAACAAGAGTAGCTCAAATTTTCTTCC
CAACTACAGGAGTAGGTAAAGGTGGTTGGTATCGCTCGCTTTCTAGTACGAGCGATGGTACAGAAAAAGCTAATAATAGT
TTTCGTATTAAAGGAGGACTCAAAGCTTTTGAGGAACCAATGGCAATTACGGGTAATTTAATTATTCCAGTTTATGACCC
TCAAGGAACGGGAATTGTTGCGGCAGATCCTTGCCTACCTCGTGTTGTGGGAGAAACAGACCGACAAACTTATTGTTTAC
CATTTGGAGCTTGTCTTAATTCTGATGGGTCAATCGATCAAAATAAAGAGAATCACAGTGGGTTTGAAACACAAACTGGT
ACTAATTGCCCAGTAGGGGCTTCTGAATGTAATAAAAACGTTATTGGCTCTGGTATTCGTAGCGTAACATTTGTACCAAC
GGAGGATAACCCACCTATGACTAATAGTTGTGGGAAATTAAAGCTGTCTGGTAATGAGCAAGGGACTGGACAGTGGCAAT
GTACGAGTCATTTAGTTCCTACGCGTTGGTATGAGCGTTATCGTTAA

Domains

Predicted by InterproScan.

von Willebrand factor type A domain (VWA_2)

(51-102)

Neisseria PilC beta-propeller domain (T4P_PilY1)

(699-948)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]