Detailed information    

experimental Experimentally validated

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   SGL_RS07945 Genome accession   NC_000911
Coordinates   1296993..1297499 (-) Length   168 a.a.
NCBI ID   WP_010872381.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.


Genomic Context

Location: 1291993..1302499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS07920 (slr1814) - 1292215..1292679 (+) 465 WP_010872376.1 DUF29 domain-containing protein -
  SGL_RS07925 (slr1815) - 1292820..1293356 (+) 537 WP_010872377.1 class I SAM-dependent methyltransferase -
  SGL_RS07930 (slr1816) - 1293398..1295551 (+) 2154 WP_010872378.1 hypothetical protein -
  SGL_RS07935 (sll1696) - 1295568..1296380 (-) 813 WP_010872379.1 hypothetical protein -
  SGL_RS07940 (sll1695) - 1296382..1296894 (-) 513 WP_010872380.1 type IV pilin protein -
  SGL_RS07945 (sll1694) pilA/pilA1 1296993..1297499 (-) 507 WP_010872381.1 type IV pilin protein Machinery gene
  SGL_RS07950 (sll1693) - 1297643..1298965 (-) 1323 WP_010872382.1 class I SAM-dependent methyltransferase -
  SGL_RS07955 (slr1818) - 1299120..1299725 (+) 606 WP_010872383.1 hypothetical protein -
  SGL_RS07960 (sll1692) - 1299729..1300166 (-) 438 WP_010872384.1 DUF29 domain-containing protein -
  SGL_RS07965 (sll1691) - 1300159..1300611 (-) 453 WP_010872385.1 DUF4351 domain-containing protein -
  SGL_RS07970 (slr1819) - 1300661..1301656 (+) 996 WP_010872386.1 pentapeptide repeat-containing protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17573.69 Da        Isoelectric Point: 4.4065

>NTDB_id=1415 SGL_RS07945 WP_010872381.1 1296993..1297499(-) (pilA/pilA1) [Synechocystis sp. PCC 6803]
MASNFKFKLLSQLSKKRAEGGFTLIELLVVVIIIGVLAAIALPNLLGQVGKARESEAKSTIGALNRAQQGYFTEKGTFAT
DTETLEVPAPDGNFFSFAVNTADNTEAIQDATALNWEADGTRSMSGGTFYDSGTRAFSTVVCRAEAGSEDTPPTPGGAND
CGGAEVIK

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1415 SGL_RS07945 WP_010872381.1 1296993..1297499(-) (pilA/pilA1) [Synechocystis sp. PCC 6803]
ATGGCTAGTAATTTTAAATTCAAACTCCTCTCTCAACTCTCCAAAAAACGGGCAGAAGGTGGTTTCACCTTAATCGAACT
GCTGGTTGTTGTAATTATTATCGGTGTATTGGCTGCTATTGCTCTGCCTAACCTCTTGGGTCAAGTTGGTAAAGCACGGG
AGTCCGAAGCTAAATCCACTATCGGTGCTTTGAACCGTGCCCAACAAGGTTATTTTACTGAGAAGGGTACGTTTGCAACC
GATACGGAAACGCTTGAAGTCCCAGCTCCTGATGGTAATTTCTTTAGTTTTGCAGTTAACACTGCTGACAACACAGAAGC
TATCCAAGACGCAACGGCATTGAATTGGGAGGCTGACGGCACCCGCTCCATGTCAGGTGGAACGTTTTACGATTCAGGCA
CCCGCGCCTTCAGTACTGTTGTTTGTCGGGCTGAAGCAGGTAGTGAAGATACTCCCCCAACCCCAGGAGGGGCTAATGAT
TGTGGTGGTGCTGAAGTAATTAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(17-42)

Type IV pilin-like G and H, putative (Pilin_GH)

(54-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]