Detailed information    

experimental Experimentally validated

Overview

Name   pilX   Type   Machinery gene
Locus tag   NMV_RS07470 Genome accession   NC_017501
Coordinates   1474310..1474783 (-) Length   157 a.a.
NCBI ID   WP_173277181.1    Uniprot ID   -
Organism   Neisseria meningitidis 8013     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The pilX mutants had a 13.2-fold reduced transformability, which was statistically significant.


Genomic Context

Location: 1469310..1479783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS07450 (NMV_1505) yaaA 1469403..1470182 (-) 780 WP_002238459.1 peroxide stress protein YaaA -
  NMV_RS07455 (NMV_1506) dapC 1470345..1471532 (-) 1188 WP_014574099.1 succinyldiaminopimelate transaminase -
  NMV_RS07460 (NMV_1507) dut 1471609..1472061 (-) 453 WP_002222651.1 dUTP diphosphatase -
  NMV_RS07465 (NMV_1508) - 1472207..1472635 (+) 429 Protein_1386 AzlC family ABC transporter permease -
  NMV_RS07470 (NMV_1509) pilX 1474310..1474783 (-) 474 WP_173277181.1 PilX family type IV pilin Machinery gene
  NMV_RS07475 (NMV_1510) pilK 1474788..1475387 (-) 600 WP_014574101.1 pilus assembly PilX family protein Machinery gene
  NMV_RS07480 (NMV_1511) pilJ 1475366..1476331 (-) 966 WP_014574102.1 PilW family protein Machinery gene
  NMV_RS07485 (NMV_1512) pilV 1476328..1476948 (-) 621 WP_014574103.1 type IV pilus modification protein PilV Machinery gene
  NMV_RS07490 (NMV_1513) pilH 1476981..1477646 (-) 666 WP_014574104.1 GspH/FimT family pseudopilin Machinery gene
  NMV_RS07495 (NMV_1514) dnaB 1477955..1479361 (-) 1407 WP_002219448.1 replicative DNA helicase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17571.32 Da        Isoelectric Point: 9.6160

>NTDB_id=1088 NMV_RS07470 WP_173277181.1 1474310..1474783(-) (pilX) [Neisseria meningitidis 8013]
MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTEMVGINNISKQFILKNPLDDNQTIKSKLERFVSGYKM
NPKIAEKYNVSVHFVNKEKPRAYSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1088 NMV_RS07470 WP_173277181.1 1474310..1474783(-) (pilX) [Neisseria meningitidis 8013]
ATGGAACAAAAAGGGTTTACATTGATTGAGATGATGATAGTCGTCGCGATACTCGGCATTATCAGCGTCATTGCCATACC
TTCTTATCAAAGTTATATTGAAAAAGGCTATCAGTCCCAGCTTTATACGGAGATGGTCGGTATCAACAATATTTCCAAAC
AGTTTATTTTGAAAAATCCCCTGGACGATAATCAGACCATCAAGAGCAAACTGGAAAGATTTGTCTCAGGCTATAAGATG
AATCCGAAAATTGCCGAAAAATATAATGTTTCGGTGCATTTTGTCAATAAGGAAAAACCAAGGGCATACAGCTTGGTCGG
CGTTCCAAAGACGGGGACGGGTTATACTTTGTCGGTATGGATGAACAGCGTGGGCGACGGATACAAATGCCGTGATGCCG
CTTCTGCCCGAGCCCATTTGGAGACCTTGTCCTCAGATGTCGGCTGTGAAGCTTTCTCTAATCGTAAAAAATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-25)

Minor type IV pilin, PilX (Pilin_PilX)

(31-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilL Neisseria gonorrhoeae MS11

85.987

100

0.86

Multiple sequence alignment    



References

[1] Ana Cehovin et al. (2011) Testing the vaccine potential of PilV, PilX and ComP, minor subunits of Neisseria meningitidis type IV pili. Vaccine 29(40):6858-65. [PMID: 21803096]
[2] Daniel R Brown et al. (2010) Systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type IV pili in Neisseria meningitidis. Infection And Immunity 78(7):3053-63. [PMID: 20439474]
[3] Sophie Hélaine et al. (2005) PilX, a pilus-associated protein essential for bacterial aggregation, is a key to pilus-facilitated attachment of Neisseria meningitidis to human cells. Molecular Microbiology 55(1):65-77. [PMID: 15612917]