Detailed information    

experimental Experimentally validated

Overview

Name   pilV   Type   Regulator
Locus tag   NMV_RS07485 Genome accession   NC_017501
Coordinates   1476328..1476948 (-) Length   206 a.a.
NCBI ID   WP_014574103.1    Uniprot ID   -
Organism   Neisseria meningitidis 8013     
Function   repress natural transformation   
Competence regulation

Function

The pilV mutant displayed a 2.4-fold-higher transformability than the WT strain.


Genomic Context

Location: 1471328..1481948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS07460 (NMV_1507) dut 1471609..1472061 (-) 453 WP_002222651.1 dUTP diphosphatase -
  NMV_RS07465 (NMV_1508) - 1472207..1472635 (+) 429 Protein_1386 AzlC family ABC transporter permease -
  NMV_RS07470 (NMV_1509) pilX 1474310..1474783 (-) 474 WP_173277181.1 PilX family type IV pilin Machinery gene
  NMV_RS07475 (NMV_1510) pilK 1474788..1475387 (-) 600 WP_014574101.1 pilus assembly PilX family protein Machinery gene
  NMV_RS07480 (NMV_1511) pilJ 1475366..1476331 (-) 966 WP_014574102.1 PilW family protein Machinery gene
  NMV_RS07485 (NMV_1512) pilV 1476328..1476948 (-) 621 WP_014574103.1 type IV pilus modification protein PilV Regulator
  NMV_RS07490 (NMV_1513) pilH 1476981..1477646 (-) 666 WP_014574104.1 GspH/FimT family pseudopilin Machinery gene
  NMV_RS07495 (NMV_1514) dnaB 1477955..1479361 (-) 1407 WP_002219448.1 replicative DNA helicase -
  NMV_RS07500 (NMV_1515) - 1479524..1480111 (+) 588 WP_002239681.1 superoxide dismutase -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 22348.39 Da        Isoelectric Point: 5.6951

>NTDB_id=1087 NMV_RS07485 WP_014574103.1 1476328..1476948(-) (pilV) [Neisseria meningitidis 8013]
MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKN
YNLYMGNHTLPHTLSAVDGDFAIDAMKTKGQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGKAPTLSGNAFSSNCDN
KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1087 NMV_RS07485 WP_014574103.1 1476328..1476948(-) (pilV) [Neisseria meningitidis 8013]
ATGAAGAATAATGATTGCTTCCGCCTGAAAAATCCCCAGTCCGGTATGGCGCTGATAGAAGTCTTGGTTGCTATGCTCGT
TCTGACCATCGGTATTTTGGCACTATTGTCCGTACAGTTGCGGACAGTCGCTTCCGTCAGGGAGGCGGAGACGCAAACCA
TCGTCAGCCAAATCACGCAAAACCTGATGGAGGGAATGTTGATGAATCCGACCATTGATTCGGACAGCAACAAGAAAAAC
TATAATCTTTACATGGGAAACCATACACTACCGCATACACTATCAGCTGTGGATGGCGATTTTGCGATTGATGCCATGAA
AACTAAGGGGCAATTGGCAGAGGCACAATTGAAGAGATTTAGTTATGAGCTGAAAAATGCCTTGCCGGATGCGGCAGCCA
TCCATTACGCCGTCTGCAAGGATTCGTCGGGTAAAGCGCCGACATTGTCCGGCAATGCTTTTTCTTCAAATTGCGACAAT
AAGGCAAACGGGGATACTTTAATTAAAGTATTGTGGGTAAATGATTCGGCAGGGGATTCGGATATTTCCCGTACGAATCT
TGAGGTGAGCGGCGACAATATCGTATATACTTATCAGGCAAGGGTCGGAGGTCGGGAATGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV/pilI Neisseria gonorrhoeae MS11

83.981

100

0.856

Multiple sequence alignment    



References

[1] Ana Cehovin et al. (2011) Testing the vaccine potential of PilV, PilX and ComP, minor subunits of Neisseria meningitidis type IV pili. Vaccine 29(40):6858-65. [PMID: 21803096]
[2] Daniel R Brown et al. (2010) Systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type IV pili in Neisseria meningitidis. Infection And Immunity 78(7):3053-63. [PMID: 20439474]