Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SM130_RS16990 Genome accession   NZ_CP139348
Coordinates   3689743..3691446 (-) Length   567 a.a.
NCBI ID   WP_102824889.1    Uniprot ID   A0A2N8S1P5
Organism   Stutzerimonas stutzeri strain KC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3684743..3696446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM130_RS16960 (SM130_16960) - 3684866..3685903 (+) 1038 WP_102824895.1 NADP(H)-dependent aldo-keto reductase -
  SM130_RS16965 (SM130_16965) - 3685968..3686648 (+) 681 WP_102824894.1 energy-coupling factor ABC transporter permease -
  SM130_RS16970 (SM130_16970) yacG 3686699..3686893 (-) 195 WP_102824893.1 DNA gyrase inhibitor YacG -
  SM130_RS16975 (SM130_16975) coaE 3686890..3687498 (-) 609 WP_102824892.1 dephospho-CoA kinase -
  SM130_RS16980 (SM130_16980) pilD 3687650..3688519 (-) 870 WP_102824891.1 A24 family peptidase Machinery gene
  SM130_RS16985 (SM130_16985) pilC 3688520..3689740 (-) 1221 WP_102824890.1 type II secretion system F family protein Machinery gene
  SM130_RS16990 (SM130_16990) pilB 3689743..3691446 (-) 1704 WP_102824889.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SM130_RS16995 (SM130_16995) pilA 3691815..3692000 (+) 186 WP_102824888.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  SM130_RS17000 (SM130_17000) - 3692015..3692461 (+) 447 WP_146029737.1 hypothetical protein -
  SM130_RS17005 (SM130_17005) - 3692532..3692711 (+) 180 WP_102824887.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SM130_RS17010 (SM130_17010) - 3693109..3693918 (+) 810 WP_219725296.1 hypothetical protein -
  SM130_RS17015 (SM130_17015) - 3693920..3694756 (+) 837 WP_181019250.1 hypothetical protein -
  SM130_RS17020 (SM130_17020) - 3694753..3695649 (+) 897 WP_102824886.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62419.60 Da        Isoelectric Point: 5.8082

>NTDB_id=909043 SM130_RS16990 WP_102824889.1 3689743..3691446(-) (pilB) [Stutzerimonas stutzeri strain KC]
MNENAPLGGLARQLVLAGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKGRVLGDLAAEQFGVPFFDLAALDKETQPKD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATTALDGLGDSDLD
GLDIEITGETRDDDTPHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAKMGIDALGYEEEQKELYLTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKTEVEVPRDVLLKEGFP
EKRIGTFKIFGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIAVQARRDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=909043 SM130_RS16990 WP_102824889.1 3689743..3691446(-) (pilB) [Stutzerimonas stutzeri strain KC]
ATGAACGAAAACGCACCCCTCGGCGGCTTGGCCCGCCAACTGGTTCTGGCAGGTCAGCTCGACGAAAAGATTGCTCAGCA
GGCTCAGCAACAGGCACTACGAAACCAGGTGCCGTTGGTTACATGGCTGGTTCAGAACAAATTGGTCAAGGGGAGGGTGC
TGGGTGATCTCGCCGCTGAGCAGTTCGGCGTTCCGTTCTTCGATCTGGCGGCGCTGGATAAGGAGACGCAGCCGAAAGAT
CTGGTGAGTGAAAAGCTGGTGCGTCAGCACCGCGCATTGCCGTTACAGAGGCGTGGCAACAAGCTCTATGTGGCGGTTTC
TGACCCTACCAATCACCAAGCGATAAGCGACATCCAATTCAATACTGGACTGTCGGTTGAGGCCGTGCTGGTCGAGGACG
ACAAGCTTGGCGAAGCCATCGAGAAGTTTTTCGAGTCGGCGACTACTGCACTAGACGGGCTGGGTGATTCCGACCTGGAT
GGTCTGGATATCGAGATCACCGGGGAGACGAGGGACGACGATACGCCGCACAACGAGGCTGACGATGCGCCGGTCGTGCG
CTTCGTCAATAAGATGTTGCTTGATGCCATCAAGGGCGGTTCGTCCGACCTGCACTTCGAACCCTATGAAAAGACCTATC
GGGTGCGTTTTCGTACCGATGGCATGTTGCACGAGGTTGCGCGCCCGCCGGTGCAGTTGGTCAGTCGGATATCCGCGCGT
TTGAAGGTCATGGCCAGCCTCGATATCGCTGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGGTGTCCAAGAC
AAAATCGATCGATTTCCGCGTTAACACCCTGCCTACGCTATGGGGTGAAAAGATCGTCATGCGTATTCTGGATTCTGCCA
GCGCCAAAATGGGTATTGACGCCTTGGGCTATGAGGAAGAGCAGAAAGAACTCTATCTGACGGCCCTCAAGCAGCCACAG
GGCATGATTCTTGTTACCGGGCCGACGGGTTCCGGCAAGACTGTATCGCTGTATACCGGCCTCAATATTCTCAATACGGT
CGATGTAAACATCTCAACGGCCGAAGATCCGGTTGAGATCAATTTGGAAGGCATCAACCAAGTCAACGTAAACCCAAAGC
AGGGCATGGACTTCGCCCAAGCTCTGCGCGCTTTCCTGCGGCAGGACCCTGACATCATCATGGTTGGGGAAATTCGGGAC
TTGGAAACCGCTGAAATCGCCATCAAGGCCGCCCAGACTGGTCATATGGTGATGTCCACGCTGCATACCAATAGTGCGGC
GGAGACGTTGACCCGCCTTCGTAATATGGGTGTGCCGGCGTTCAATATCGCAACCTCAGTGAACCTAATTATCGCTCAGC
GGCTGGCTCGAAAATTATGTCCATCCTGCAAGACAGAGGTGGAGGTGCCACGGGACGTGTTGCTAAAAGAGGGATTTCCC
GAGAAAAGAATCGGTACCTTCAAGATTTTTGGTCCGGTGGGGTGTGATAGCTGCAAGGGTGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGCTTATCATGGAGGAAGGCAATTCCATCGACATCGCCGTGC
AGGCGCGCAGAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTTAAAGCCATGAATGGTGTTACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8S1P5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.292

99.647

0.591
  pilB Acinetobacter baumannii D1279779

59.253

99.118

0.587
  pilB Legionella pneumophila strain ERS1305867

54.991

100

0.554
  pilF Neisseria gonorrhoeae MS11

50.829

95.767

0.487
  pilB Vibrio campbellii strain DS40M4

50.091

96.825

0.485
  pilB Vibrio cholerae strain A1552

53.162

89.242

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

53.267

89.065

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

37.419

100

0.409
  pilF Thermus thermophilus HB27

37.676

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.298

0.367