6107

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 535977..536617 Replicon chromosome
Accession NC_006351
Organism Burkholderia pseudomallei K96243

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag BPS_RS20815 Protein ID WP_009893919.1
Coordinates 535977..536156 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q63NA5
Locus tag BPS_RS20820 Protein ID WP_004552310.1
Coordinates 536201..536617 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BPS_RS20795 (BPSS0386) 532668..533243 + 576 WP_004552315.1 recombinase family protein -
BPS_RS20800 (BPSS0387) 533314..534429 - 1116 WP_004552314.1 DUF3396 domain-containing protein -
BPS_RS20805 (BPSS0388) 534439..535173 - 735 WP_004552313.1 VRR-NUC domain-containing protein -
BPS_RS20810 (BPSS0389) 535170..535697 - 528 WP_004552312.1 PAAR domain-containing protein -
BPS_RS20815 (BPSS0390) 535977..536156 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
BPS_RS20820 (BPSS0391) 536201..536617 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BPS_RS20825 (BPSS0391A) 537046..537543 - 498 WP_004552308.1 hypothetical protein -
BPS_RS20830 (BPSS0392) 538161..538568 - 408 Protein_443 DDE-type integrase/transposase/recombinase -
BPS_RS20835 (BPSS0394) 539214..539498 - 285 WP_004525420.1 BrnA antitoxin family protein -
BPS_RS20840 (BPSS0395) 539482..539655 - 174 WP_009939402.1 BrnT family toxin -
BPS_RS20845 (BPSS0396) 540323..540598 + 276 WP_004552388.1 bacteriophage protein Gp49 -
BPS_RS20850 (BPSS0397) 540608..540739 + 132 WP_009923452.1 bacteriophage protein Gp48 -
BPS_RS20855 (BPSS0398) 540875..541096 + 222 WP_004552390.1 hypothetical protein -
BPS_RS20860 541063..541362 + 300 WP_160469016.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 516820..546139 29319
flank IS/Tn - - 538161..538481 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T6107 WP_009893919.1 NC_006351:535977-536156 [Burkholderia pseudomallei K96243]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T6107 NC_006351:535977-536156 [Burkholderia pseudomallei K96243]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGCCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15120.06 Da        Isoelectric Point: 4.7025

>AT6107 WP_004552310.1 NC_006351:536201-536617 [Burkholderia pseudomallei K96243]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT6107 NC_006351:536201-536617 [Burkholderia pseudomallei K96243]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GAGAAGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTCGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T3593 Acinetobacter baumannii ATCC 17978

78.431

98.077

0.769
T875 Sinorhizobium meliloti 1021

61.017

100

0.61
T10122 Paracoccus phage vB_PbeS_Pben1

35.385

96.296

0.341
T10168 Streptococcus pneumoniae TIGR4

30

100

0.305
T6283 Pseudomonas aeruginosa PA1

30.508

100

0.305

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT3593 Acinetobacter baumannii ATCC 17978

45.522

97.101

0.442
AT875 Sinorhizobium meliloti 1021

40

94.203

0.377

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
AlphaFold DB Q63NA5

References

(1) Ashley J Winter et al. (2018) The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. The Journal of Biological Chemistry 293(50):19429-19440. [PubMed:30337369] experimental

(2) Aaron Butt et al. (2014) The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation. The Biochemical Journal 459(2):333-44. [PubMed:24502667] experimental

(3) Aaron Butt et al. (2013) Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei. FEMS Microbiology Letters 338(1):86-94. [PubMed:23082999] experimental

(4) Brittany N Ross et al. (2019) Evaluating the role of Burkholderia pseudomallei K96243 toxins BPSS0390, BPSS0395, and BPSS1584 in persistent infection. Cellular Microbiology 21(12):e13096. [PubMed:31400259] experimental


experimental experimental literature